Urine NMR metabolomic study on biochemical activities to investigate the effect of P. betle extract on obese rats
The present studies are to evaluate the ability of PB to induce weight loss and urine metabolite profile of Piper betle L. (PB) leaf extracts using metabolomics approach. Dried PB leaves were extracted with ethanol 70% and the studies were performed in different groups of rats fed with high fat (HFD...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Springer
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/82683/ http://psasir.upm.edu.my/id/eprint/82683/1/Urine%20NMR%20metabolomic%20study%20.pdf |
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| author | Abdul Ghani, Zuleen Delina Fasya Ab Rashid, Ahmad Hazri Shaari, Khozirah Chik, Zamri |
| author_facet | Abdul Ghani, Zuleen Delina Fasya Ab Rashid, Ahmad Hazri Shaari, Khozirah Chik, Zamri |
| author_sort | Abdul Ghani, Zuleen Delina Fasya |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present studies are to evaluate the ability of PB to induce weight loss and urine metabolite profile of Piper betle L. (PB) leaf extracts using metabolomics approach. Dried PB leaves were extracted with ethanol 70% and the studies were performed in different groups of rats fed with high fat (HFD) and normal diet (ND). Then, fed with the PB extract with 100, 300, and 500 mg/kg and two negative control groups given water (WTR). The body weights were monitored and evaluated. Urine was collected and 1H NMR-based metabolomics approach was used to detect the metabolite changes. Results showed that PB-treated group demonstrated inhibition of body weight gain. The trajectory of urine metabolites showed that PB-treated group gave the different distribution from week 12 to 16 compared with the control groups. In 1H NMR metabolomic approach analysis, the urine metabolites gave the best separation in principle component 1 and 3, with 40.0% and 9.56% of the total variation. Shared and unique structures (SUS) plot model showed that higher concentration PB-treated group was characterized by high level of indole-3-acetate, aspartate, methanol, histidine, and creatine, thus caused an increased the metabolic function and maintaining the body weight of the animals treated. |
| first_indexed | 2025-11-15T12:27:35Z |
| format | Article |
| id | upm-82683 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:27:35Z |
| publishDate | 2019 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-826832021-12-06T02:05:45Z http://psasir.upm.edu.my/id/eprint/82683/ Urine NMR metabolomic study on biochemical activities to investigate the effect of P. betle extract on obese rats Abdul Ghani, Zuleen Delina Fasya Ab Rashid, Ahmad Hazri Shaari, Khozirah Chik, Zamri The present studies are to evaluate the ability of PB to induce weight loss and urine metabolite profile of Piper betle L. (PB) leaf extracts using metabolomics approach. Dried PB leaves were extracted with ethanol 70% and the studies were performed in different groups of rats fed with high fat (HFD) and normal diet (ND). Then, fed with the PB extract with 100, 300, and 500 mg/kg and two negative control groups given water (WTR). The body weights were monitored and evaluated. Urine was collected and 1H NMR-based metabolomics approach was used to detect the metabolite changes. Results showed that PB-treated group demonstrated inhibition of body weight gain. The trajectory of urine metabolites showed that PB-treated group gave the different distribution from week 12 to 16 compared with the control groups. In 1H NMR metabolomic approach analysis, the urine metabolites gave the best separation in principle component 1 and 3, with 40.0% and 9.56% of the total variation. Shared and unique structures (SUS) plot model showed that higher concentration PB-treated group was characterized by high level of indole-3-acetate, aspartate, methanol, histidine, and creatine, thus caused an increased the metabolic function and maintaining the body weight of the animals treated. Springer 2019-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/82683/1/Urine%20NMR%20metabolomic%20study%20.pdf Abdul Ghani, Zuleen Delina Fasya and Ab Rashid, Ahmad Hazri and Shaari, Khozirah and Chik, Zamri (2019) Urine NMR metabolomic study on biochemical activities to investigate the effect of P. betle extract on obese rats. Applied Biochemistry and Biotechnology, 189 (1). pp. 690-708. ISSN 0273-2289; ESSN: 1559-0291 https://link.springer.com/article/10.1007/s12010-019-03042-w 10.1007/s12010-019-03042-w |
| spellingShingle | Abdul Ghani, Zuleen Delina Fasya Ab Rashid, Ahmad Hazri Shaari, Khozirah Chik, Zamri Urine NMR metabolomic study on biochemical activities to investigate the effect of P. betle extract on obese rats |
| title | Urine NMR metabolomic study on biochemical activities to investigate the effect of P. betle extract on obese rats |
| title_full | Urine NMR metabolomic study on biochemical activities to investigate the effect of P. betle extract on obese rats |
| title_fullStr | Urine NMR metabolomic study on biochemical activities to investigate the effect of P. betle extract on obese rats |
| title_full_unstemmed | Urine NMR metabolomic study on biochemical activities to investigate the effect of P. betle extract on obese rats |
| title_short | Urine NMR metabolomic study on biochemical activities to investigate the effect of P. betle extract on obese rats |
| title_sort | urine nmr metabolomic study on biochemical activities to investigate the effect of p. betle extract on obese rats |
| url | http://psasir.upm.edu.my/id/eprint/82683/ http://psasir.upm.edu.my/id/eprint/82683/ http://psasir.upm.edu.my/id/eprint/82683/ http://psasir.upm.edu.my/id/eprint/82683/1/Urine%20NMR%20metabolomic%20study%20.pdf |