Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line
Background: Combination chemotherapy of anticancer drugs is extensively being researched since it could reduce multidrug resistance and side effects as a result of lower dosage of each drug. In this study, we evaluated the effects of doxorubicin-loaded (Dox-ACNP), thymoquinone-loaded (TQ-ACNP) and a...
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| Format: | Article |
| Language: | English |
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Frontiers Media
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/82661/ http://psasir.upm.edu.my/id/eprint/82661/1/Ultrastructural%20changes%20and%20antitumor%20.pdf |
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| author | Ibiyeye, Kehinde Muibat Nordin, Norshariza Ajat, Mokrish Zakaria Zuki, Abu Bakar |
| author_facet | Ibiyeye, Kehinde Muibat Nordin, Norshariza Ajat, Mokrish Zakaria Zuki, Abu Bakar |
| author_sort | Ibiyeye, Kehinde Muibat |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Combination chemotherapy of anticancer drugs is extensively being researched since it could reduce multidrug resistance and side effects as a result of lower dosage of each drug. In this study, we evaluated the effects of doxorubicin-loaded (Dox-ACNP), thymoquinone-loaded (TQ-ACNP) and a combined doxorubicin/thymoquinone-loaded cockle shell-derived aragonite calcium carbonate nanoparticles (Dox/TQ-ACNP) on breast cancer cell line and compared with their free drugs counterpart. Methods: Cell viability using MTT assay, apoptosis with Annexin V-PI kit, morphological changes using contrast light microscope, scanning electron microscope and transmission electron microscope, cell cycle analysis, invasion assay, and scratch assay were carried out. The cell viability was evaluated in breast cancer cell line (MDA MB231), normal breast cells (MDF10A) and normal fibroblast (3T3). Results: MDA MB231 IC50 dosages of drug-loaded nanoparticle were not toxic to the normal cells. The combination therapy showed enhanced apoptosis, reduction in cellular migration and invasion when compared to the single drug-loaded nanoparticle and the free drugs. Scanning electron microscope showed presence of cell shrinkage, cell membrane blebbing, while transmission electron microscope showed nuclear fragmentation, disruption of cell membrane, apoptotic bodies, and disruption of mitochondrial cistern. Conclusion: The results from this study showed that the combined drug-loaded cockle shell-derived aragonite calcium carbonate nanoparticles (Dox/TQ-ACNP) showed higher efficacy in breast cancer cells at lower dose of doxorubicin and thymoquinone. |
| first_indexed | 2025-11-15T12:27:30Z |
| format | Article |
| id | upm-82661 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:27:30Z |
| publishDate | 2019 |
| publisher | Frontiers Media |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-826612021-06-05T23:37:03Z http://psasir.upm.edu.my/id/eprint/82661/ Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line Ibiyeye, Kehinde Muibat Nordin, Norshariza Ajat, Mokrish Zakaria Zuki, Abu Bakar Background: Combination chemotherapy of anticancer drugs is extensively being researched since it could reduce multidrug resistance and side effects as a result of lower dosage of each drug. In this study, we evaluated the effects of doxorubicin-loaded (Dox-ACNP), thymoquinone-loaded (TQ-ACNP) and a combined doxorubicin/thymoquinone-loaded cockle shell-derived aragonite calcium carbonate nanoparticles (Dox/TQ-ACNP) on breast cancer cell line and compared with their free drugs counterpart. Methods: Cell viability using MTT assay, apoptosis with Annexin V-PI kit, morphological changes using contrast light microscope, scanning electron microscope and transmission electron microscope, cell cycle analysis, invasion assay, and scratch assay were carried out. The cell viability was evaluated in breast cancer cell line (MDA MB231), normal breast cells (MDF10A) and normal fibroblast (3T3). Results: MDA MB231 IC50 dosages of drug-loaded nanoparticle were not toxic to the normal cells. The combination therapy showed enhanced apoptosis, reduction in cellular migration and invasion when compared to the single drug-loaded nanoparticle and the free drugs. Scanning electron microscope showed presence of cell shrinkage, cell membrane blebbing, while transmission electron microscope showed nuclear fragmentation, disruption of cell membrane, apoptotic bodies, and disruption of mitochondrial cistern. Conclusion: The results from this study showed that the combined drug-loaded cockle shell-derived aragonite calcium carbonate nanoparticles (Dox/TQ-ACNP) showed higher efficacy in breast cancer cells at lower dose of doxorubicin and thymoquinone. Frontiers Media 2019-07 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/82661/1/Ultrastructural%20changes%20and%20antitumor%20.pdf Ibiyeye, Kehinde Muibat and Nordin, Norshariza and Ajat, Mokrish and Zakaria Zuki, Abu Bakar (2019) Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line. art. no. 599. pp. 1-14. ISSN 2234-943X https://pubmed.ncbi.nlm.nih.gov/31334120/ 10.3389/fonc.2019.00599 |
| spellingShingle | Ibiyeye, Kehinde Muibat Nordin, Norshariza Ajat, Mokrish Zakaria Zuki, Abu Bakar Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line |
| title | Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line |
| title_full | Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line |
| title_fullStr | Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line |
| title_full_unstemmed | Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line |
| title_short | Ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded CaCO3 nanoparticles on breast cancer cell line |
| title_sort | ultrastructural changes and antitumor effects of doxorubicin/ thymoquinone-loaded caco3 nanoparticles on breast cancer cell line |
| url | http://psasir.upm.edu.my/id/eprint/82661/ http://psasir.upm.edu.my/id/eprint/82661/ http://psasir.upm.edu.my/id/eprint/82661/ http://psasir.upm.edu.my/id/eprint/82661/1/Ultrastructural%20changes%20and%20antitumor%20.pdf |