The influence of serum‐supplemented culture media in a transwell migration assay
Our work cautions against the use of serum-supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FB...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/82524/ http://psasir.upm.edu.my/id/eprint/82524/1/The%20influence%20of%20serum.pdf |
| _version_ | 1848859299108880384 |
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| author | Omar Zaki, Siti Sarah Kanesan, Livashini Leong, Ming Yeh Danielle Vidyadaran, Sharmili |
| author_facet | Omar Zaki, Siti Sarah Kanesan, Livashini Leong, Ming Yeh Danielle Vidyadaran, Sharmili |
| author_sort | Omar Zaki, Siti Sarah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Our work cautions against the use of serum-supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FBS-supplemented media in the absence of a gradient also resulted in notable microglia migration. Serum can therefore confound the interpretation of a transwell migration assay when another chemoattractant is used. |
| first_indexed | 2025-11-15T12:27:08Z |
| format | Article |
| id | upm-82524 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:27:08Z |
| publishDate | 2019 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-825242020-10-16T20:20:41Z http://psasir.upm.edu.my/id/eprint/82524/ The influence of serum‐supplemented culture media in a transwell migration assay Omar Zaki, Siti Sarah Kanesan, Livashini Leong, Ming Yeh Danielle Vidyadaran, Sharmili Our work cautions against the use of serum-supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FBS-supplemented media in the absence of a gradient also resulted in notable microglia migration. Serum can therefore confound the interpretation of a transwell migration assay when another chemoattractant is used. Wiley 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/82524/1/The%20influence%20of%20serum.pdf Omar Zaki, Siti Sarah and Kanesan, Livashini and Leong, Ming Yeh Danielle and Vidyadaran, Sharmili (2019) The influence of serum‐supplemented culture media in a transwell migration assay. Cell Biology International, 43 (10). pp. 1201-1204. ISSN 1065-6995; ESSN: 1095-8355 10.1002/cbin.11122 |
| spellingShingle | Omar Zaki, Siti Sarah Kanesan, Livashini Leong, Ming Yeh Danielle Vidyadaran, Sharmili The influence of serum‐supplemented culture media in a transwell migration assay |
| title | The influence of serum‐supplemented culture media in a transwell migration assay |
| title_full | The influence of serum‐supplemented culture media in a transwell migration assay |
| title_fullStr | The influence of serum‐supplemented culture media in a transwell migration assay |
| title_full_unstemmed | The influence of serum‐supplemented culture media in a transwell migration assay |
| title_short | The influence of serum‐supplemented culture media in a transwell migration assay |
| title_sort | influence of serum‐supplemented culture media in a transwell migration assay |
| url | http://psasir.upm.edu.my/id/eprint/82524/ http://psasir.upm.edu.my/id/eprint/82524/ http://psasir.upm.edu.my/id/eprint/82524/1/The%20influence%20of%20serum.pdf |