Resistance of native bacteria isolated from activated sludge towards iron and manganese

A study was conducted to observe the resistance of native isolated bacteria towards selected heavy metals (iron; Fe and manganese; Mn) in a separate exposure. Isolated bacteria were evaluated by culturing them in nutrient broth medium that contained approximately 3...

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Main Authors: Marsidi, Nuratiqah, Abu Hasan, Hassimi, Halmi, Mohd Izuan Effendi, Sheikh Abdullah, Siti Rozaimah
Format: Article
Language:English
Published: The Malaysian Analytical Sciences Society 2019
Online Access:http://psasir.upm.edu.my/id/eprint/81996/
http://psasir.upm.edu.my/id/eprint/81996/1/Resistance%20of%20native%20bacteria%20isolated%20from%20activated%20sludge%20towards%20iron%20and%20manganese.pdf
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author Marsidi, Nuratiqah
Abu Hasan, Hassimi
Halmi, Mohd Izuan Effendi
Sheikh Abdullah, Siti Rozaimah
author_facet Marsidi, Nuratiqah
Abu Hasan, Hassimi
Halmi, Mohd Izuan Effendi
Sheikh Abdullah, Siti Rozaimah
author_sort Marsidi, Nuratiqah
building UPM Institutional Repository
collection Online Access
description A study was conducted to observe the resistance of native isolated bacteria towards selected heavy metals (iron; Fe and manganese; Mn) in a separate exposure. Isolated bacteria were evaluated by culturing them in nutrient broth medium that contained approximately 3 ×10⁶CFU/mL bacteria with different initial concentrations (0, 50, 100 and 200 mg/L). Result showed that a plate with bacterial growth indicated bacterial resistance, which was verified based on CFU/mL. At 0 mg/L, bacteria grew well on the plate with Fe and Mn. The bacterial number began to decrease at 50 and 100 mg/L for Fe and at 200 mg/L for Mn. Only a few colonies survived (isolate AM2) the toxicity of highFe amount; the isolated bacteria almost showed no growth along the plate. AM2, AM3 and AM4 presented resistance to Mn until 200 mg/L, but not AM6. The bacteria showed no growth at 100 and 200 mg/L. Thus, the Fe and Mn concentrations that can be applied during acclimatisation ranged from 0 mg/L to 200 mg/L with isolated AM2 and AM4 for Fe and Mn removal.
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spelling upm-819962021-08-13T00:42:33Z http://psasir.upm.edu.my/id/eprint/81996/ Resistance of native bacteria isolated from activated sludge towards iron and manganese Marsidi, Nuratiqah Abu Hasan, Hassimi Halmi, Mohd Izuan Effendi Sheikh Abdullah, Siti Rozaimah A study was conducted to observe the resistance of native isolated bacteria towards selected heavy metals (iron; Fe and manganese; Mn) in a separate exposure. Isolated bacteria were evaluated by culturing them in nutrient broth medium that contained approximately 3 ×10⁶CFU/mL bacteria with different initial concentrations (0, 50, 100 and 200 mg/L). Result showed that a plate with bacterial growth indicated bacterial resistance, which was verified based on CFU/mL. At 0 mg/L, bacteria grew well on the plate with Fe and Mn. The bacterial number began to decrease at 50 and 100 mg/L for Fe and at 200 mg/L for Mn. Only a few colonies survived (isolate AM2) the toxicity of highFe amount; the isolated bacteria almost showed no growth along the plate. AM2, AM3 and AM4 presented resistance to Mn until 200 mg/L, but not AM6. The bacteria showed no growth at 100 and 200 mg/L. Thus, the Fe and Mn concentrations that can be applied during acclimatisation ranged from 0 mg/L to 200 mg/L with isolated AM2 and AM4 for Fe and Mn removal. The Malaysian Analytical Sciences Society 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81996/1/Resistance%20of%20native%20bacteria%20isolated%20from%20activated%20sludge%20towards%20iron%20and%20manganese.pdf Marsidi, Nuratiqah and Abu Hasan, Hassimi and Halmi, Mohd Izuan Effendi and Sheikh Abdullah, Siti Rozaimah (2019) Resistance of native bacteria isolated from activated sludge towards iron and manganese. Malaysian Journal of Analytical Sciences, 23 (1). pp. 131-137. ISSN 1394 -2506 https://mjas.analis.com.my/mjas/v23_n1/html/23_1_16.html 10.17576/mjas-2019-2301-16
spellingShingle Marsidi, Nuratiqah
Abu Hasan, Hassimi
Halmi, Mohd Izuan Effendi
Sheikh Abdullah, Siti Rozaimah
Resistance of native bacteria isolated from activated sludge towards iron and manganese
title Resistance of native bacteria isolated from activated sludge towards iron and manganese
title_full Resistance of native bacteria isolated from activated sludge towards iron and manganese
title_fullStr Resistance of native bacteria isolated from activated sludge towards iron and manganese
title_full_unstemmed Resistance of native bacteria isolated from activated sludge towards iron and manganese
title_short Resistance of native bacteria isolated from activated sludge towards iron and manganese
title_sort resistance of native bacteria isolated from activated sludge towards iron and manganese
url http://psasir.upm.edu.my/id/eprint/81996/
http://psasir.upm.edu.my/id/eprint/81996/
http://psasir.upm.edu.my/id/eprint/81996/
http://psasir.upm.edu.my/id/eprint/81996/1/Resistance%20of%20native%20bacteria%20isolated%20from%20activated%20sludge%20towards%20iron%20and%20manganese.pdf