Resistance of native bacteria isolated from activated sludge towards iron and manganese
A study was conducted to observe the resistance of native isolated bacteria towards selected heavy metals (iron; Fe and manganese; Mn) in a separate exposure. Isolated bacteria were evaluated by culturing them in nutrient broth medium that contained approximately 3...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Malaysian Analytical Sciences Society
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81996/ http://psasir.upm.edu.my/id/eprint/81996/1/Resistance%20of%20native%20bacteria%20isolated%20from%20activated%20sludge%20towards%20iron%20and%20manganese.pdf |
| Summary: | A study was conducted to observe the resistance of native isolated bacteria towards selected heavy metals (iron; Fe and manganese; Mn) in a separate exposure. Isolated bacteria were evaluated by culturing them in nutrient broth medium that contained approximately 3 ×10⁶CFU/mL bacteria with different initial concentrations (0, 50, 100 and 200 mg/L). Result showed that a plate with bacterial growth indicated bacterial resistance, which was verified based on CFU/mL. At 0 mg/L, bacteria grew well on the plate with Fe and Mn. The bacterial number began to decrease at 50 and 100 mg/L for Fe and at 200 mg/L for Mn. Only a few colonies survived (isolate AM2) the toxicity of highFe amount; the isolated bacteria almost showed no growth along the plate. AM2, AM3 and AM4 presented resistance to Mn until 200 mg/L, but not AM6. The bacteria showed no growth at 100 and 200 mg/L. Thus, the Fe and Mn concentrations that can be applied during acclimatisation ranged from 0 mg/L to 200 mg/L with isolated AM2 and AM4 for Fe and Mn removal. |
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