Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia
Vibrio infections are common among marine fish and lead to serious problems in the aquaculture sector. This study reports a recent occurrence of Vibrio species (spp.) isolated from cultured groupers in Peninsular Malaysia using the gyrB and pyrH genes. A total of 147 Vibrio strains were successfully...
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| Format: | Article |
| Language: | English |
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Wiley
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81944/ http://psasir.upm.edu.my/id/eprint/81944/1/Recent%20update%20on%20the%20prevalence%20of%20vibrio%20species%20among%20cultured%20grouper%20in%20Peninsular%20Malaysia.pdf |
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| author | Nor Zulkiply, Amalina Zulperi, Dzarifah Azmai, Mohammad Noor Amal Yusof, Mohd Termizi Saad, Mohd Zamri Al-Saari, Nurhidayu Tanaka, Mami Mino, Sayaka Sawabe, Tomoo Md Yasin, Ina Salwany |
| author_facet | Nor Zulkiply, Amalina Zulperi, Dzarifah Azmai, Mohammad Noor Amal Yusof, Mohd Termizi Saad, Mohd Zamri Al-Saari, Nurhidayu Tanaka, Mami Mino, Sayaka Sawabe, Tomoo Md Yasin, Ina Salwany |
| author_sort | Nor Zulkiply, Amalina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Vibrio infections are common among marine fish and lead to serious problems in the aquaculture sector. This study reports a recent occurrence of Vibrio species (spp.) isolated from cultured groupers in Peninsular Malaysia using the gyrB and pyrH genes. A total of 147 Vibrio strains were successfully isolated from 77 (64%) groupers using culture method and subjected to gyrB and pyrH sequencing for species identification and confirmation. Results showed that 89% of Vibrio strains were identified and clustered to six groups of Vibrio spp., while 11% were not clustered to any Vibrio spp. using the gyrB sequences. Meanwhile, by analysis of the pyrH sequences all the 147 Vibrio strains (100%) were successfully identified and clustered into 11 groups of Vibrio spp., including the gyrB non‐identified strains. The pyrH gene provides a better resolution for identification of Vibrio spp. compared with the gyrB gene. Thus, the pyrH gene was more suitable for a rapid determination of Vibrio spp. distribution in Peninsular Malaysia. Using the pyrH gene, our study found higher prevalence of Vibrio vulnificus (33%), V. alginolyticus (24%) and V. parahaemolyticus (22%), followed by V. rotiferianus (5%), V. harveyi (3%), V. tubiashii (2%), V. campbellii (2%), V. ponticus (1%), V. diabolicus (1%), V. owensii (1%) and others Vibrio sp. (7%). Thus, the results of this study revealed that the occurrence of pathogenic vibrios among grouper fish is still high in Malaysian aquaculture. In addition, the pyrH gene was proved as a suitable marker for rapid identification of Vibrio species compared with the gyrB gene. |
| first_indexed | 2025-11-15T12:25:37Z |
| format | Article |
| id | upm-81944 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:25:37Z |
| publishDate | 2019 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-819442020-09-04T02:43:39Z http://psasir.upm.edu.my/id/eprint/81944/ Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia Nor Zulkiply, Amalina Zulperi, Dzarifah Azmai, Mohammad Noor Amal Yusof, Mohd Termizi Saad, Mohd Zamri Al-Saari, Nurhidayu Tanaka, Mami Mino, Sayaka Sawabe, Tomoo Md Yasin, Ina Salwany Vibrio infections are common among marine fish and lead to serious problems in the aquaculture sector. This study reports a recent occurrence of Vibrio species (spp.) isolated from cultured groupers in Peninsular Malaysia using the gyrB and pyrH genes. A total of 147 Vibrio strains were successfully isolated from 77 (64%) groupers using culture method and subjected to gyrB and pyrH sequencing for species identification and confirmation. Results showed that 89% of Vibrio strains were identified and clustered to six groups of Vibrio spp., while 11% were not clustered to any Vibrio spp. using the gyrB sequences. Meanwhile, by analysis of the pyrH sequences all the 147 Vibrio strains (100%) were successfully identified and clustered into 11 groups of Vibrio spp., including the gyrB non‐identified strains. The pyrH gene provides a better resolution for identification of Vibrio spp. compared with the gyrB gene. Thus, the pyrH gene was more suitable for a rapid determination of Vibrio spp. distribution in Peninsular Malaysia. Using the pyrH gene, our study found higher prevalence of Vibrio vulnificus (33%), V. alginolyticus (24%) and V. parahaemolyticus (22%), followed by V. rotiferianus (5%), V. harveyi (3%), V. tubiashii (2%), V. campbellii (2%), V. ponticus (1%), V. diabolicus (1%), V. owensii (1%) and others Vibrio sp. (7%). Thus, the results of this study revealed that the occurrence of pathogenic vibrios among grouper fish is still high in Malaysian aquaculture. In addition, the pyrH gene was proved as a suitable marker for rapid identification of Vibrio species compared with the gyrB gene. Wiley 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81944/1/Recent%20update%20on%20the%20prevalence%20of%20vibrio%20species%20among%20cultured%20grouper%20in%20Peninsular%20Malaysia.pdf Nor Zulkiply, Amalina and Zulperi, Dzarifah and Azmai, Mohammad Noor Amal and Yusof, Mohd Termizi and Saad, Mohd Zamri and Al-Saari, Nurhidayu and Tanaka, Mami and Mino, Sayaka and Sawabe, Tomoo and Md Yasin, Ina Salwany (2019) Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia. Aquaculture Research, 50 (11). pp. 3202-3210. ISSN 1355-557X; ESSN: 1365-2109 http://onlinelibrary.wiley.com/journal/10.1111/ 10.1111/are.14275 |
| spellingShingle | Nor Zulkiply, Amalina Zulperi, Dzarifah Azmai, Mohammad Noor Amal Yusof, Mohd Termizi Saad, Mohd Zamri Al-Saari, Nurhidayu Tanaka, Mami Mino, Sayaka Sawabe, Tomoo Md Yasin, Ina Salwany Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia |
| title | Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia |
| title_full | Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia |
| title_fullStr | Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia |
| title_full_unstemmed | Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia |
| title_short | Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia |
| title_sort | recent update on the prevalence of vibrio species among cultured grouper in peninsular malaysia |
| url | http://psasir.upm.edu.my/id/eprint/81944/ http://psasir.upm.edu.my/id/eprint/81944/ http://psasir.upm.edu.my/id/eprint/81944/ http://psasir.upm.edu.my/id/eprint/81944/1/Recent%20update%20on%20the%20prevalence%20of%20vibrio%20species%20among%20cultured%20grouper%20in%20Peninsular%20Malaysia.pdf |