Purification of a bacteriocin‐like inhibitory substance derived from pediococcus acidilactici Kp10 by an aqueous micellar two‐phase system
A polymer–salt aqueous two-phase system (ATPS) consisting of polyethylene–glycol (PEG) with sodium citrate was developed for direct recovery of a bacteriocin-like inhibitory substance (BLIS) from a culture of Pediococcus acidilactici Kp10. The influences of phase composition, tie-line length (TLL),...
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| Format: | Article |
| Language: | English |
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Wiley
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81893/ http://psasir.upm.edu.my/id/eprint/81893/1/Purification%20of%20a%20bacteriocin.pdf |
| _version_ | 1848859193633669120 |
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| author | Jamaluddin, Norfariza Ariff, Arbakariya Faizal Wong, Fadzlie Wong |
| author_facet | Jamaluddin, Norfariza Ariff, Arbakariya Faizal Wong, Fadzlie Wong |
| author_sort | Jamaluddin, Norfariza |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A polymer–salt aqueous two-phase system (ATPS) consisting of polyethylene–glycol (PEG) with sodium citrate was developed for direct recovery of a bacteriocin-like inhibitory substance (BLIS) from a culture of Pediococcus acidilactici Kp10. The influences of phase composition, tie-line length (TLL), volume ratio (VR), crude sample loading, pH and sodium chloride (NaCl) on the partition behaviour of BLIS was investigated. Under optimum conditions of ATPS, the purification of BLIS was achieved at 26.5% PEG (8000)/11% sodium citrate with a TLL of 46.38% (w/w), VR of 1.8, and 1.8% crude load at pH 7 without the presence of NaCl. BLIS from P. acidilactici Kp10 was successfully purified by the ATPS up to 8.43-fold with a yield of 81.18%. Given that the operation of ATPS is simple, environmentally friendly and cost-effective, as it requires only salts and PEG, it may have potential for industrial applications in the recovery of BLIS from fermentation broth. |
| first_indexed | 2025-11-15T12:25:27Z |
| format | Article |
| id | upm-81893 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:25:27Z |
| publishDate | 2014 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-818932021-09-29T02:04:46Z http://psasir.upm.edu.my/id/eprint/81893/ Purification of a bacteriocin‐like inhibitory substance derived from pediococcus acidilactici Kp10 by an aqueous micellar two‐phase system Jamaluddin, Norfariza Ariff, Arbakariya Faizal Wong, Fadzlie Wong A polymer–salt aqueous two-phase system (ATPS) consisting of polyethylene–glycol (PEG) with sodium citrate was developed for direct recovery of a bacteriocin-like inhibitory substance (BLIS) from a culture of Pediococcus acidilactici Kp10. The influences of phase composition, tie-line length (TLL), volume ratio (VR), crude sample loading, pH and sodium chloride (NaCl) on the partition behaviour of BLIS was investigated. Under optimum conditions of ATPS, the purification of BLIS was achieved at 26.5% PEG (8000)/11% sodium citrate with a TLL of 46.38% (w/w), VR of 1.8, and 1.8% crude load at pH 7 without the presence of NaCl. BLIS from P. acidilactici Kp10 was successfully purified by the ATPS up to 8.43-fold with a yield of 81.18%. Given that the operation of ATPS is simple, environmentally friendly and cost-effective, as it requires only salts and PEG, it may have potential for industrial applications in the recovery of BLIS from fermentation broth. Wiley 2014-05 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81893/1/Purification%20of%20a%20bacteriocin.pdf Jamaluddin, Norfariza and Ariff, Arbakariya and Faizal Wong, Fadzlie Wong (2014) Purification of a bacteriocin‐like inhibitory substance derived from pediococcus acidilactici Kp10 by an aqueous micellar two‐phase system. Biotechnology Progress, 35 (1). pp. 93-100. ISSN 8756-7938; ESSN: 1520-6033 https://aiche.onlinelibrary.wiley.com/doi/10.1002/btpr.2719 10.1016/j.foodchem.2013.11.019 |
| spellingShingle | Jamaluddin, Norfariza Ariff, Arbakariya Faizal Wong, Fadzlie Wong Purification of a bacteriocin‐like inhibitory substance derived from pediococcus acidilactici Kp10 by an aqueous micellar two‐phase system |
| title | Purification of a bacteriocin‐like inhibitory substance derived from pediococcus acidilactici Kp10 by an aqueous micellar two‐phase system |
| title_full | Purification of a bacteriocin‐like inhibitory substance derived from pediococcus acidilactici Kp10 by an aqueous micellar two‐phase system |
| title_fullStr | Purification of a bacteriocin‐like inhibitory substance derived from pediococcus acidilactici Kp10 by an aqueous micellar two‐phase system |
| title_full_unstemmed | Purification of a bacteriocin‐like inhibitory substance derived from pediococcus acidilactici Kp10 by an aqueous micellar two‐phase system |
| title_short | Purification of a bacteriocin‐like inhibitory substance derived from pediococcus acidilactici Kp10 by an aqueous micellar two‐phase system |
| title_sort | purification of a bacteriocin‐like inhibitory substance derived from pediococcus acidilactici kp10 by an aqueous micellar two‐phase system |
| url | http://psasir.upm.edu.my/id/eprint/81893/ http://psasir.upm.edu.my/id/eprint/81893/ http://psasir.upm.edu.my/id/eprint/81893/ http://psasir.upm.edu.my/id/eprint/81893/1/Purification%20of%20a%20bacteriocin.pdf |