Production, optimization and characterisation of chitosanase of Bacillus sp. and its applications in nanotechnology
Chitosanases is a class of enzymes which hydrolyse chitosan, a natural biopolymer consisting of d-glucosamine in various degrees. In this study, chitosanase producing Bacillus sp. was isolated from soil sample. Chitosanase production was optimized using response surface methodology and the produced...
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| Format: | Article |
| Language: | English |
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Springer
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81851/ http://psasir.upm.edu.my/id/eprint/81851/1/Production%2C%20optimization%20and%20characterisation%20of%20chitosanase%20of%20Bacillus%20sp.%20and%20its%20applications%20in%20nanotechnology.pdf |
| _version_ | 1848859185957044224 |
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| author | N., Shobana Samrot, Anthony V. Subbiah, Suresh Kumar G., Narendrakumar |
| author_facet | N., Shobana Samrot, Anthony V. Subbiah, Suresh Kumar G., Narendrakumar |
| author_sort | N., Shobana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Chitosanases is a class of enzymes which hydrolyse chitosan, a natural biopolymer consisting of d-glucosamine in various degrees. In this study, chitosanase producing Bacillus sp. was isolated from soil sample. Chitosanase production was optimized using response surface methodology and the produced chitosanase was characterized. The crude enzyme was found to possess antibacterial and antifungal activity. Chitosanase enzyme was used for trimming chitosan based polymeric nanoparticles produced using sodium trimetaphosphate chelator. Chitosanase enzyme was also utilized for synthesis of silver nanoparticles which were then characterized by UV–Vis, FTIR, SEM, TEM and AFM. The produced nanoparticles were checked for antibacterial and antifungal activity. |
| first_indexed | 2025-11-15T12:25:20Z |
| format | Article |
| id | upm-81851 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:25:20Z |
| publishDate | 2019 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-818512020-08-28T01:16:04Z http://psasir.upm.edu.my/id/eprint/81851/ Production, optimization and characterisation of chitosanase of Bacillus sp. and its applications in nanotechnology N., Shobana Samrot, Anthony V. Subbiah, Suresh Kumar G., Narendrakumar Chitosanases is a class of enzymes which hydrolyse chitosan, a natural biopolymer consisting of d-glucosamine in various degrees. In this study, chitosanase producing Bacillus sp. was isolated from soil sample. Chitosanase production was optimized using response surface methodology and the produced chitosanase was characterized. The crude enzyme was found to possess antibacterial and antifungal activity. Chitosanase enzyme was used for trimming chitosan based polymeric nanoparticles produced using sodium trimetaphosphate chelator. Chitosanase enzyme was also utilized for synthesis of silver nanoparticles which were then characterized by UV–Vis, FTIR, SEM, TEM and AFM. The produced nanoparticles were checked for antibacterial and antifungal activity. Springer 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81851/1/Production%2C%20optimization%20and%20characterisation%20of%20chitosanase%20of%20Bacillus%20sp.%20and%20its%20applications%20in%20nanotechnology.pdf N., Shobana and Samrot, Anthony V. and Subbiah, Suresh Kumar and G., Narendrakumar (2019) Production, optimization and characterisation of chitosanase of Bacillus sp. and its applications in nanotechnology. Journal of Cluster Science, 30. pp. 607-620. ISSN 1040-7278; ESSN: 1572-8862 https://link.springer.com/article/10.1007/s10876-019-01520-z 10.1007/s10876-019-01520-z |
| spellingShingle | N., Shobana Samrot, Anthony V. Subbiah, Suresh Kumar G., Narendrakumar Production, optimization and characterisation of chitosanase of Bacillus sp. and its applications in nanotechnology |
| title | Production, optimization and characterisation of chitosanase of Bacillus sp. and its applications in nanotechnology |
| title_full | Production, optimization and characterisation of chitosanase of Bacillus sp. and its applications in nanotechnology |
| title_fullStr | Production, optimization and characterisation of chitosanase of Bacillus sp. and its applications in nanotechnology |
| title_full_unstemmed | Production, optimization and characterisation of chitosanase of Bacillus sp. and its applications in nanotechnology |
| title_short | Production, optimization and characterisation of chitosanase of Bacillus sp. and its applications in nanotechnology |
| title_sort | production, optimization and characterisation of chitosanase of bacillus sp. and its applications in nanotechnology |
| url | http://psasir.upm.edu.my/id/eprint/81851/ http://psasir.upm.edu.my/id/eprint/81851/ http://psasir.upm.edu.my/id/eprint/81851/ http://psasir.upm.edu.my/id/eprint/81851/1/Production%2C%20optimization%20and%20characterisation%20of%20chitosanase%20of%20Bacillus%20sp.%20and%20its%20applications%20in%20nanotechnology.pdf |