Production of polyhydroxybutyrate from oil palm empty fruit bunch (OPEFB) hydrolysates by Bacillus cereus suaeda B-001
Polyhydroxybutyrate (PHB) is a biodegradable polymer accumulated in intracellular granules by numerousbacteria. Its physical and chemical characteristics are like those of petrochemical plastics. PHB is producedmainly by gram-negative bacteria such asRalstonia eutropha, which have lipopolysaccharide...
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| Language: | English |
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Elsevier
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81850/ http://psasir.upm.edu.my/id/eprint/81850/1/Production%20of%20polyhydroxybutyrate%20from%20oil%20palm%20empty%20fruit%20bunch%20%28OPEFB%29%20hydrolysates%20by%20Bacillus%20cereus%20suaeda%20B-001.pdf |
| _version_ | 1848859185661345792 |
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| author | Yustinah Nurul Hidayat Alamsyah, Rizal Roslan, Ahmad Muhaimin Hermansyah, Heri Gozan, Misri |
| author_facet | Yustinah Nurul Hidayat Alamsyah, Rizal Roslan, Ahmad Muhaimin Hermansyah, Heri Gozan, Misri |
| author_sort | Yustinah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Polyhydroxybutyrate (PHB) is a biodegradable polymer accumulated in intracellular granules by numerousbacteria. Its physical and chemical characteristics are like those of petrochemical plastics. PHB is producedmainly by gram-negative bacteria such asRalstonia eutropha, which have lipopolysaccharides that co-purify withthe PHB and cause immunogenic reactions, limiting their use for biomedical applications. PHB produced fromgram-positive bacteria such asBacillus spp.do not have lipopolysaccharides, which makes it suitable for bio-medical application. The aim of this work was to evaluate the ability ofBacillus cereus suaedaB-001 to accu-mulate PHB using oil palm empty fruit bunch (OPEFB) hydrolysate as the sole carbon source, comparing it tocommercial glucose as the control. OPEFB was chemically pre-treated using an acid-hydrolysed process bysulphuric acid and neutralized by a NaOH solution to obtain reducing sugars. PHB biopolyester accumulated to43.1% of cell dry weight with glucose at 15 g/L as the sole carbon source, and PHB accumulated to 55.4% of celldry weight using OPEFB hydrolysates at 20 g/L. The conversion of OPEFB acid hydrolysates to PHB using thegram-positive bacteriaBacillus cereus suaedaB-001 has not been reported. |
| first_indexed | 2025-11-15T12:25:19Z |
| format | Article |
| id | upm-81850 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:25:19Z |
| publishDate | 2019 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-818502020-08-27T08:26:06Z http://psasir.upm.edu.my/id/eprint/81850/ Production of polyhydroxybutyrate from oil palm empty fruit bunch (OPEFB) hydrolysates by Bacillus cereus suaeda B-001 Yustinah Nurul Hidayat Alamsyah, Rizal Roslan, Ahmad Muhaimin Hermansyah, Heri Gozan, Misri Polyhydroxybutyrate (PHB) is a biodegradable polymer accumulated in intracellular granules by numerousbacteria. Its physical and chemical characteristics are like those of petrochemical plastics. PHB is producedmainly by gram-negative bacteria such asRalstonia eutropha, which have lipopolysaccharides that co-purify withthe PHB and cause immunogenic reactions, limiting their use for biomedical applications. PHB produced fromgram-positive bacteria such asBacillus spp.do not have lipopolysaccharides, which makes it suitable for bio-medical application. The aim of this work was to evaluate the ability ofBacillus cereus suaedaB-001 to accu-mulate PHB using oil palm empty fruit bunch (OPEFB) hydrolysate as the sole carbon source, comparing it tocommercial glucose as the control. OPEFB was chemically pre-treated using an acid-hydrolysed process bysulphuric acid and neutralized by a NaOH solution to obtain reducing sugars. PHB biopolyester accumulated to43.1% of cell dry weight with glucose at 15 g/L as the sole carbon source, and PHB accumulated to 55.4% of celldry weight using OPEFB hydrolysates at 20 g/L. The conversion of OPEFB acid hydrolysates to PHB using thegram-positive bacteriaBacillus cereus suaedaB-001 has not been reported. Elsevier 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81850/1/Production%20of%20polyhydroxybutyrate%20from%20oil%20palm%20empty%20fruit%20bunch%20%28OPEFB%29%20hydrolysates%20by%20Bacillus%20cereus%20suaeda%20B-001.pdf Yustinah and Nurul Hidayat and Alamsyah, Rizal and Roslan, Ahmad Muhaimin and Hermansyah, Heri and Gozan, Misri (2019) Production of polyhydroxybutyrate from oil palm empty fruit bunch (OPEFB) hydrolysates by Bacillus cereus suaeda B-001. Biocatalysis and Agricultural Biotechnology, 18 (2019). pp. 1-7. ISSN 1878-8181 https://reader.elsevier.com/reader/sd/pii/S1878818118304316?token=B7DB0EA31223FEDF7C354F8CDE5E1B1342EC668F3FEEC2B12FD71EE33E08286E8FABBFE0FF28916F62531728455BB531 10.1016/j.bcab.2019.01.057 |
| spellingShingle | Yustinah Nurul Hidayat Alamsyah, Rizal Roslan, Ahmad Muhaimin Hermansyah, Heri Gozan, Misri Production of polyhydroxybutyrate from oil palm empty fruit bunch (OPEFB) hydrolysates by Bacillus cereus suaeda B-001 |
| title | Production of polyhydroxybutyrate from oil palm empty fruit bunch (OPEFB) hydrolysates by Bacillus cereus suaeda B-001 |
| title_full | Production of polyhydroxybutyrate from oil palm empty fruit bunch (OPEFB) hydrolysates by Bacillus cereus suaeda B-001 |
| title_fullStr | Production of polyhydroxybutyrate from oil palm empty fruit bunch (OPEFB) hydrolysates by Bacillus cereus suaeda B-001 |
| title_full_unstemmed | Production of polyhydroxybutyrate from oil palm empty fruit bunch (OPEFB) hydrolysates by Bacillus cereus suaeda B-001 |
| title_short | Production of polyhydroxybutyrate from oil palm empty fruit bunch (OPEFB) hydrolysates by Bacillus cereus suaeda B-001 |
| title_sort | production of polyhydroxybutyrate from oil palm empty fruit bunch (opefb) hydrolysates by bacillus cereus suaeda b-001 |
| url | http://psasir.upm.edu.my/id/eprint/81850/ http://psasir.upm.edu.my/id/eprint/81850/ http://psasir.upm.edu.my/id/eprint/81850/ http://psasir.upm.edu.my/id/eprint/81850/1/Production%20of%20polyhydroxybutyrate%20from%20oil%20palm%20empty%20fruit%20bunch%20%28OPEFB%29%20hydrolysates%20by%20Bacillus%20cereus%20suaeda%20B-001.pdf |