Production of ligninolytic enzymes by Coptotermes curvignathus gut bacteria
Maximum utilization of lignocellulosic biomass is contingent upon degrading the recalcitrant lignin polymer. Conventional methods employed in delignification require high inputs of energy and chemicals, resulting in the release of highly toxic effluents. The ability of gut flora of Coptotermes curvi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Walter de Gruyter GmbH
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81849/ http://psasir.upm.edu.my/id/eprint/81849/1/Production%20of%20ligninolytic%20enzymes%20by%20Coptotermes%20curvignathus%20gut%20bacteria.pdf |
| _version_ | 1848859185385570304 |
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| author | Ayeronfe, Fadilah Kassim, Angzzas Hung, Patricia Ishak, Nadiah Sharfina Syarifah Aripin, Ashuvila |
| author_facet | Ayeronfe, Fadilah Kassim, Angzzas Hung, Patricia Ishak, Nadiah Sharfina Syarifah Aripin, Ashuvila |
| author_sort | Ayeronfe, Fadilah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Maximum utilization of lignocellulosic biomass is contingent upon degrading the recalcitrant lignin polymer. Conventional methods employed in delignification require high inputs of energy and chemicals, resulting in the release of highly toxic effluents. The ability of gut flora of Coptotermes curvignathus in lignin degradation was investigated in this study. Production of ligninolytic enzymes was done in an aerated submerged fermentation system with kraft lignin as sole carbon source. The degradation experiment was carried out for 7 days at 30 °C, pH 7. Three potential lignin degraders identified as Bacillus sp., Lysinibacillus sp. and Acinetobacter sp. were successfully isolated. The bacterial growth and secretion of extracellular ligninolytic enzymes confirmed metabolism of kraft lignin by the identified strains. Lysinibacillus sp., a novel lignin degrader showed highest manganese peroxidase (76.36 ± 15.74 U/L) and laccase activity (70.67 ± 16.82 U/L) after 7 and 6 days of incubation respectively, while maximal activity of lignin peroxidase (262.49 ± 0.92 U/L) was recorded after 7 days in culture supernatants of Bacillus sp. With respect to the activity of the secreted enzymes, the lignin degrading potential of these bacterial strains can be explored in the valorisations of lignocellulosic biomass in industrial processes such as pulping, bioethanol production, fine chemicals and materials synthesis. |
| first_indexed | 2025-11-15T12:25:19Z |
| format | Article |
| id | upm-81849 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:25:19Z |
| publishDate | 2019 |
| publisher | Walter de Gruyter GmbH |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-818492020-08-27T02:23:22Z http://psasir.upm.edu.my/id/eprint/81849/ Production of ligninolytic enzymes by Coptotermes curvignathus gut bacteria Ayeronfe, Fadilah Kassim, Angzzas Hung, Patricia Ishak, Nadiah Sharfina Syarifah Aripin, Ashuvila Maximum utilization of lignocellulosic biomass is contingent upon degrading the recalcitrant lignin polymer. Conventional methods employed in delignification require high inputs of energy and chemicals, resulting in the release of highly toxic effluents. The ability of gut flora of Coptotermes curvignathus in lignin degradation was investigated in this study. Production of ligninolytic enzymes was done in an aerated submerged fermentation system with kraft lignin as sole carbon source. The degradation experiment was carried out for 7 days at 30 °C, pH 7. Three potential lignin degraders identified as Bacillus sp., Lysinibacillus sp. and Acinetobacter sp. were successfully isolated. The bacterial growth and secretion of extracellular ligninolytic enzymes confirmed metabolism of kraft lignin by the identified strains. Lysinibacillus sp., a novel lignin degrader showed highest manganese peroxidase (76.36 ± 15.74 U/L) and laccase activity (70.67 ± 16.82 U/L) after 7 and 6 days of incubation respectively, while maximal activity of lignin peroxidase (262.49 ± 0.92 U/L) was recorded after 7 days in culture supernatants of Bacillus sp. With respect to the activity of the secreted enzymes, the lignin degrading potential of these bacterial strains can be explored in the valorisations of lignocellulosic biomass in industrial processes such as pulping, bioethanol production, fine chemicals and materials synthesis. Walter de Gruyter GmbH 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81849/1/Production%20of%20ligninolytic%20enzymes%20by%20Coptotermes%20curvignathus%20gut%20bacteria.pdf Ayeronfe, Fadilah and Kassim, Angzzas and Hung, Patricia and Ishak, Nadiah and Sharfina Syarifah and Aripin, Ashuvila (2019) Production of ligninolytic enzymes by Coptotermes curvignathus gut bacteria. Environmental and Climate Technologies, 23 (1). pp. 111-121. ISSN 16915208 https://content.sciendo.com/configurable/contentpage/journals$002frtuect$002f23$002f1$002farticle-p111.xml 10.2478/rtuect-2019-0008 |
| spellingShingle | Ayeronfe, Fadilah Kassim, Angzzas Hung, Patricia Ishak, Nadiah Sharfina Syarifah Aripin, Ashuvila Production of ligninolytic enzymes by Coptotermes curvignathus gut bacteria |
| title | Production of ligninolytic enzymes by Coptotermes curvignathus gut bacteria |
| title_full | Production of ligninolytic enzymes by Coptotermes curvignathus gut bacteria |
| title_fullStr | Production of ligninolytic enzymes by Coptotermes curvignathus gut bacteria |
| title_full_unstemmed | Production of ligninolytic enzymes by Coptotermes curvignathus gut bacteria |
| title_short | Production of ligninolytic enzymes by Coptotermes curvignathus gut bacteria |
| title_sort | production of ligninolytic enzymes by coptotermes curvignathus gut bacteria |
| url | http://psasir.upm.edu.my/id/eprint/81849/ http://psasir.upm.edu.my/id/eprint/81849/ http://psasir.upm.edu.my/id/eprint/81849/ http://psasir.upm.edu.my/id/eprint/81849/1/Production%20of%20ligninolytic%20enzymes%20by%20Coptotermes%20curvignathus%20gut%20bacteria.pdf |