Phylogenetic relationships among several freshwater fishes (Family: Cyprinidae) in Malaysia inferred from partial sequencing of the Cytochrome b mitochondrial DNA (mtDNA) gene
The phylogenetic relationships among 23 species of Malaysian freshwater fishes in the family Cyprinidae was inferred by partial sequencing of the Cytochrome b (Cyt b) mitochondrial gene. Samples were collected from various localities in Sarawak, Sabah and Peninsular Malaysia. The inferred phylogeny...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/8182/ http://psasir.upm.edu.my/id/eprint/8182/1/Phylogenetic%20Relationships%20among%20Several%20Freshwater%20Fishes%20%28Family%20Cyprinidae%29%20in%20Malaysia%20Inferred%20from%20Partial%20Sequencing%20of%20the%20Cytochrome%20b%20Mitochondrial%20DNA%20%28mtDNA%29%20Gene%20.pdf |
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| author | Esa, Yuzine Japning, Jeffrine Rovie Ryan Abdul Rahim, Khairul Adha Siraj, Siti Shapor Daud, Siti Khalijah Tan, Soon Guan Stephen, Sungan |
| author_facet | Esa, Yuzine Japning, Jeffrine Rovie Ryan Abdul Rahim, Khairul Adha Siraj, Siti Shapor Daud, Siti Khalijah Tan, Soon Guan Stephen, Sungan |
| author_sort | Esa, Yuzine |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The phylogenetic relationships among 23 species of Malaysian freshwater fishes in the family Cyprinidae was inferred by partial sequencing of the Cytochrome b (Cyt b) mitochondrial gene. Samples were collected from various localities in Sarawak, Sabah and Peninsular Malaysia. The inferred phylogeny appeared to match major groupings currently recognized in the taxonomy but no support was evident for nearly all the higher level groupings. Nevertheless, some interesting insights were gained in relation to the phylogenetic relationships among some genera under study. Meanwhile, the phylogenetic relationship among Mahseer fishes (genus Tor and Neolissochilus) were poorly resolved using the current data alone, but the taxonomic revision of other genera particularly for the genus Puntius could improve this. The current study suggest that P. binotatus and P. sealei could be representative of the genus Puntius, while any other species identified as belonging to the genus Puntius should cluster with this group. The study also revealed that two morphologically similar Barbonymus species (namely, B. gonionotus and B. schwanenfeldii) were phylogenetically distinct (13.0% K2P genetic distance). This indicated that a taxonomic revision of B. gonionotus would be required from its current position within the genus Barbonymus. The results of the current study also revealed two interesting findings for Hampala; (1) the Borneo endemic Hampala forms are distinct from the widespread H. macrolepidota, and (2) two distinct lineage were evident in H. bimaculata from Sarawak. In general, the sequence analysis of the cytochrome b mtDNA region has been proven to be useful for assessing phylogenetic relationships among indigenous freshwater fishes in Malaysia. |
| first_indexed | 2025-11-15T07:33:05Z |
| format | Article |
| id | upm-8182 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:33:05Z |
| publishDate | 2012 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-81822015-11-03T06:44:23Z http://psasir.upm.edu.my/id/eprint/8182/ Phylogenetic relationships among several freshwater fishes (Family: Cyprinidae) in Malaysia inferred from partial sequencing of the Cytochrome b mitochondrial DNA (mtDNA) gene Esa, Yuzine Japning, Jeffrine Rovie Ryan Abdul Rahim, Khairul Adha Siraj, Siti Shapor Daud, Siti Khalijah Tan, Soon Guan Stephen, Sungan The phylogenetic relationships among 23 species of Malaysian freshwater fishes in the family Cyprinidae was inferred by partial sequencing of the Cytochrome b (Cyt b) mitochondrial gene. Samples were collected from various localities in Sarawak, Sabah and Peninsular Malaysia. The inferred phylogeny appeared to match major groupings currently recognized in the taxonomy but no support was evident for nearly all the higher level groupings. Nevertheless, some interesting insights were gained in relation to the phylogenetic relationships among some genera under study. Meanwhile, the phylogenetic relationship among Mahseer fishes (genus Tor and Neolissochilus) were poorly resolved using the current data alone, but the taxonomic revision of other genera particularly for the genus Puntius could improve this. The current study suggest that P. binotatus and P. sealei could be representative of the genus Puntius, while any other species identified as belonging to the genus Puntius should cluster with this group. The study also revealed that two morphologically similar Barbonymus species (namely, B. gonionotus and B. schwanenfeldii) were phylogenetically distinct (13.0% K2P genetic distance). This indicated that a taxonomic revision of B. gonionotus would be required from its current position within the genus Barbonymus. The results of the current study also revealed two interesting findings for Hampala; (1) the Borneo endemic Hampala forms are distinct from the widespread H. macrolepidota, and (2) two distinct lineage were evident in H. bimaculata from Sarawak. In general, the sequence analysis of the cytochrome b mtDNA region has been proven to be useful for assessing phylogenetic relationships among indigenous freshwater fishes in Malaysia. Universiti Putra Malaysia Press 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/8182/1/Phylogenetic%20Relationships%20among%20Several%20Freshwater%20Fishes%20%28Family%20Cyprinidae%29%20in%20Malaysia%20Inferred%20from%20Partial%20Sequencing%20of%20the%20Cytochrome%20b%20Mitochondrial%20DNA%20%28mtDNA%29%20Gene%20.pdf Esa, Yuzine and Japning, Jeffrine Rovie Ryan and Abdul Rahim, Khairul Adha and Siraj, Siti Shapor and Daud, Siti Khalijah and Tan, Soon Guan and Stephen, Sungan (2012) Phylogenetic relationships among several freshwater fishes (Family: Cyprinidae) in Malaysia inferred from partial sequencing of the Cytochrome b mitochondrial DNA (mtDNA) gene. Pertanika Journal of Tropical Agricultural Science, 35 (2). pp. 307-318. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2035%20(2)%20May.%202012/14%20Pg%20307-318.pdf |
| spellingShingle | Esa, Yuzine Japning, Jeffrine Rovie Ryan Abdul Rahim, Khairul Adha Siraj, Siti Shapor Daud, Siti Khalijah Tan, Soon Guan Stephen, Sungan Phylogenetic relationships among several freshwater fishes (Family: Cyprinidae) in Malaysia inferred from partial sequencing of the Cytochrome b mitochondrial DNA (mtDNA) gene |
| title | Phylogenetic relationships among several freshwater fishes (Family: Cyprinidae) in Malaysia inferred from partial sequencing of the Cytochrome b mitochondrial DNA (mtDNA) gene |
| title_full | Phylogenetic relationships among several freshwater fishes (Family: Cyprinidae) in Malaysia inferred from partial sequencing of the Cytochrome b mitochondrial DNA (mtDNA) gene |
| title_fullStr | Phylogenetic relationships among several freshwater fishes (Family: Cyprinidae) in Malaysia inferred from partial sequencing of the Cytochrome b mitochondrial DNA (mtDNA) gene |
| title_full_unstemmed | Phylogenetic relationships among several freshwater fishes (Family: Cyprinidae) in Malaysia inferred from partial sequencing of the Cytochrome b mitochondrial DNA (mtDNA) gene |
| title_short | Phylogenetic relationships among several freshwater fishes (Family: Cyprinidae) in Malaysia inferred from partial sequencing of the Cytochrome b mitochondrial DNA (mtDNA) gene |
| title_sort | phylogenetic relationships among several freshwater fishes (family: cyprinidae) in malaysia inferred from partial sequencing of the cytochrome b mitochondrial dna (mtdna) gene |
| url | http://psasir.upm.edu.my/id/eprint/8182/ http://psasir.upm.edu.my/id/eprint/8182/ http://psasir.upm.edu.my/id/eprint/8182/1/Phylogenetic%20Relationships%20among%20Several%20Freshwater%20Fishes%20%28Family%20Cyprinidae%29%20in%20Malaysia%20Inferred%20from%20Partial%20Sequencing%20of%20the%20Cytochrome%20b%20Mitochondrial%20DNA%20%28mtDNA%29%20Gene%20.pdf |