Pathology and immunohistochemical evaluation of Vibrio alginolyticus infection in Macrobrachium rosenberbergii
Vibriosis is one of the common bacterial diseases plaguing the prawn and shrimp industry. However, the sequential histopathological changes associated with Vibrio alginolyticus-induced vibriosis is scanty in literature. This study investigated the histological alterations in groups of Macrobrachium...
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| Format: | Article |
| Language: | English |
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Springer
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81628/ http://psasir.upm.edu.my/id/eprint/81628/1/Pathology%20and%20immunohistochemical%20evaluation%20of%20Vibrio%20alginolyticus%20infection%20in%20Macrobrachium%20rosenberbergii.pdf |
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| author | Abiodun, Ajadi Abdullateef Mohd Yusoff, Md Sabri Atata, J. A. Daodu, O. B. Emikpe, B. O. |
| author_facet | Abiodun, Ajadi Abdullateef Mohd Yusoff, Md Sabri Atata, J. A. Daodu, O. B. Emikpe, B. O. |
| author_sort | Abiodun, Ajadi Abdullateef |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Vibriosis is one of the common bacterial diseases plaguing the prawn and shrimp industry. However, the sequential histopathological changes associated with Vibrio alginolyticus-induced vibriosis is scanty in literature. This study investigated the histological alterations in groups of Macrobrachium rosenbergii experimentally injected intramuscularly between the second and third abdominal segment with 50 μL of 107 CFU of V. alginolyticus. Twenty-four hours post inoculation, the organism was isolated and molecularly characterized while infected giant freshwater prawns were observed for histological alterations and immunoreactivity in different tissues due to the experimental challenge. The lesions observed in the organs include incipient haemocytic infiltration into the interstitial space of the tubules of the hepatopancreas and loss of epithelial layer, muscular necrosis and disruption of muscular layer with haemocytic infiltration, hyperplasia of epithelial cells and degeneration of the epithelium, deformed and necrotizing lamellae, and enlargement of the lamellar sinus of the gill, and the heart is characterized by localized nodular haemocytic reaction and melanization. Organs such as the heart, gill, hepatopancreas and muscle following injection of the putative bacteria showed immunohistochemical activity. Hence, histology and immunohistochemistry still remain important tools for vibriosis disease diagnoses in giant freshwater prawns. |
| first_indexed | 2025-11-15T12:24:47Z |
| format | Article |
| id | upm-81628 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:24:47Z |
| publishDate | 2019 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-816282020-10-27T21:23:33Z http://psasir.upm.edu.my/id/eprint/81628/ Pathology and immunohistochemical evaluation of Vibrio alginolyticus infection in Macrobrachium rosenberbergii Abiodun, Ajadi Abdullateef Mohd Yusoff, Md Sabri Atata, J. A. Daodu, O. B. Emikpe, B. O. Vibriosis is one of the common bacterial diseases plaguing the prawn and shrimp industry. However, the sequential histopathological changes associated with Vibrio alginolyticus-induced vibriosis is scanty in literature. This study investigated the histological alterations in groups of Macrobrachium rosenbergii experimentally injected intramuscularly between the second and third abdominal segment with 50 μL of 107 CFU of V. alginolyticus. Twenty-four hours post inoculation, the organism was isolated and molecularly characterized while infected giant freshwater prawns were observed for histological alterations and immunoreactivity in different tissues due to the experimental challenge. The lesions observed in the organs include incipient haemocytic infiltration into the interstitial space of the tubules of the hepatopancreas and loss of epithelial layer, muscular necrosis and disruption of muscular layer with haemocytic infiltration, hyperplasia of epithelial cells and degeneration of the epithelium, deformed and necrotizing lamellae, and enlargement of the lamellar sinus of the gill, and the heart is characterized by localized nodular haemocytic reaction and melanization. Organs such as the heart, gill, hepatopancreas and muscle following injection of the putative bacteria showed immunohistochemical activity. Hence, histology and immunohistochemistry still remain important tools for vibriosis disease diagnoses in giant freshwater prawns. Springer 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81628/1/Pathology%20and%20immunohistochemical%20evaluation%20of%20Vibrio%20alginolyticus%20infection%20in%20Macrobrachium%20rosenberbergii.pdf Abiodun, Ajadi Abdullateef and Mohd Yusoff, Md Sabri and Atata, J. A. and Daodu, O. B. and Emikpe, B. O. (2019) Pathology and immunohistochemical evaluation of Vibrio alginolyticus infection in Macrobrachium rosenberbergii. Comparative Clinical Pathology, 28. pp. 359-368. ISSN 1618-565X; ESSN: 1618-5641 https://link.springer.com/article/10.1007/s00580-018-2881-4 10.1007/s00580-018-2881-4 |
| spellingShingle | Abiodun, Ajadi Abdullateef Mohd Yusoff, Md Sabri Atata, J. A. Daodu, O. B. Emikpe, B. O. Pathology and immunohistochemical evaluation of Vibrio alginolyticus infection in Macrobrachium rosenberbergii |
| title | Pathology and immunohistochemical evaluation of Vibrio alginolyticus infection in Macrobrachium rosenberbergii |
| title_full | Pathology and immunohistochemical evaluation of Vibrio alginolyticus infection in Macrobrachium rosenberbergii |
| title_fullStr | Pathology and immunohistochemical evaluation of Vibrio alginolyticus infection in Macrobrachium rosenberbergii |
| title_full_unstemmed | Pathology and immunohistochemical evaluation of Vibrio alginolyticus infection in Macrobrachium rosenberbergii |
| title_short | Pathology and immunohistochemical evaluation of Vibrio alginolyticus infection in Macrobrachium rosenberbergii |
| title_sort | pathology and immunohistochemical evaluation of vibrio alginolyticus infection in macrobrachium rosenberbergii |
| url | http://psasir.upm.edu.my/id/eprint/81628/ http://psasir.upm.edu.my/id/eprint/81628/ http://psasir.upm.edu.my/id/eprint/81628/ http://psasir.upm.edu.my/id/eprint/81628/1/Pathology%20and%20immunohistochemical%20evaluation%20of%20Vibrio%20alginolyticus%20infection%20in%20Macrobrachium%20rosenberbergii.pdf |