Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology
Nigella sativa L. seed, also recognized as black cumin is a medicinal plant that possesses miraculous power of healing due to the abilities to cure various ailments. Owing to the oily properties of plant oil, the oil ester is more preferable exhibiting special features such as non-toxic, outstandin...
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| Format: | Article |
| Language: | English |
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Malaysian Society of Applied Biology
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81572/ http://psasir.upm.edu.my/id/eprint/81572/1/SATIVA.pdf |
| _version_ | 1848859138157707264 |
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| author | Azhari, Farihin Zaidan, Uswatun Hasanah Ahmad, Syahida Abd Gani, Siti Salwa |
| author_facet | Azhari, Farihin Zaidan, Uswatun Hasanah Ahmad, Syahida Abd Gani, Siti Salwa |
| author_sort | Azhari, Farihin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Nigella sativa L. seed, also recognized as black cumin is a medicinal plant that possesses miraculous power of healing due to
the abilities to cure various ailments. Owing to the oily properties of plant oil, the oil ester is more preferable exhibiting special features such as non-toxic, outstanding moisturizing action and most importantly the absence of oily texture. In this study, Nigella sativa oil-based ferulate ester was synthesized via transesterification of Nigella sativa oil (NSO) and ethyl ferulate (EF) in the presence of immobilized lipase, Lipozyme RM IM from Rhizomucor miehei. A five-level-four-factor central composite rotatable design (CCRD) from response surface methodology (RSM) was applied to study the influence of synthesis parameters: lipase dosage (50-80 mg), temperature (40-60°C), substrate ratio of NSO:EF (2.5:1-5.5:1 w/w) and time (3-7 hr) aiming for the maximum percentage yield of ester. The optimized synthesis conditions were lipase dosage of 67 mg, temperature of 56°C, substrate ratio (NSO:EF) of 4.4:1 w/w and time of 4 hr. The maximum production of ester obtained was 49.87% which is acceptable with the predicted value of 48.82%. |
| first_indexed | 2025-11-15T12:24:34Z |
| format | Article |
| id | upm-81572 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:24:34Z |
| publishDate | 2019 |
| publisher | Malaysian Society of Applied Biology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-815722021-05-06T22:56:34Z http://psasir.upm.edu.my/id/eprint/81572/ Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology Azhari, Farihin Zaidan, Uswatun Hasanah Ahmad, Syahida Abd Gani, Siti Salwa Nigella sativa L. seed, also recognized as black cumin is a medicinal plant that possesses miraculous power of healing due to the abilities to cure various ailments. Owing to the oily properties of plant oil, the oil ester is more preferable exhibiting special features such as non-toxic, outstanding moisturizing action and most importantly the absence of oily texture. In this study, Nigella sativa oil-based ferulate ester was synthesized via transesterification of Nigella sativa oil (NSO) and ethyl ferulate (EF) in the presence of immobilized lipase, Lipozyme RM IM from Rhizomucor miehei. A five-level-four-factor central composite rotatable design (CCRD) from response surface methodology (RSM) was applied to study the influence of synthesis parameters: lipase dosage (50-80 mg), temperature (40-60°C), substrate ratio of NSO:EF (2.5:1-5.5:1 w/w) and time (3-7 hr) aiming for the maximum percentage yield of ester. The optimized synthesis conditions were lipase dosage of 67 mg, temperature of 56°C, substrate ratio (NSO:EF) of 4.4:1 w/w and time of 4 hr. The maximum production of ester obtained was 49.87% which is acceptable with the predicted value of 48.82%. Malaysian Society of Applied Biology 2019-05 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81572/1/SATIVA.pdf Azhari, Farihin and Zaidan, Uswatun Hasanah and Ahmad, Syahida and Abd Gani, Siti Salwa (2019) Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology. Malaysian Applied Biology, 48 (2). pp. 27-31. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=902&catid=59:current-view&Itemid=56 |
| spellingShingle | Azhari, Farihin Zaidan, Uswatun Hasanah Ahmad, Syahida Abd Gani, Siti Salwa Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology |
| title | Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology |
| title_full | Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology |
| title_fullStr | Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology |
| title_full_unstemmed | Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology |
| title_short | Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology |
| title_sort | optimization of nigella sativa oil-based ferulate ester synthesis using response surface methodology |
| url | http://psasir.upm.edu.my/id/eprint/81572/ http://psasir.upm.edu.my/id/eprint/81572/ http://psasir.upm.edu.my/id/eprint/81572/1/SATIVA.pdf |