Nutritional composition and angiotensin converting enzyme inhibitory activity of blue lupin (Lupinus angustifolius)
AMIN BIN ISMAIL/ Chew Lye Yee// CHIN YING YEE,AZRINA BINTI AZLAN/// Gaik Theng Toh,Junus Salam Pessy The nutritional, protein and amino acid compositions of blue lupin (Lupinus angustifolius) flour were studied. The angiotensin converting enzyme (ACE) inhibitory activity of lupin protein isolate...
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| Format: | Article |
| Language: | English |
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Elsevier
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81511/ http://psasir.upm.edu.my/id/eprint/81511/1/Nutritional%20composition%20and%20angiotensin%20converting%20enzyme%20inhibitory%20activity%20of%20blue%20lupin%20%28Lupinus%20angustifolius%29.pdf |
| _version_ | 1848859121961402368 |
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| author | Chin, Ying Yee Chew, Lye Yee Toh, Gaik Theng Salampess, Junus Azlan, Azrina Ismail, Amin |
| author_facet | Chin, Ying Yee Chew, Lye Yee Toh, Gaik Theng Salampess, Junus Azlan, Azrina Ismail, Amin |
| author_sort | Chin, Ying Yee |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | AMIN BIN ISMAIL/ Chew Lye Yee// CHIN YING YEE,AZRINA BINTI AZLAN/// Gaik Theng Toh,Junus Salam Pessy
The nutritional, protein and amino acid compositions of blue lupin (Lupinus angustifolius) flour were studied. The angiotensin converting enzyme (ACE) inhibitory activity of lupin protein isolate (LPI) hydrolysates prepared using Alcalase and Flavourzyme, and the Osborne protein fractions hydrolysates prepared using Alcalase were determined. Lupin flour was high in protein (43 g/100 g) and dietary fiber (34 g/100 g) but low in carbohydrate (4.8 g/100 g) and ash (3.4 g/100 g). Results from a sequential Osborne extraction procedure showed that lupin protein was comprised of 56% globulin, 26% albumin and 19% glutelin, while prolamin was only found in trace amounts. Lupin protein was high in Lys but limiting in Met. SDS-PAGE analysis suggested that protein hydrolysis catalyzed using Alcalase was more effective than Flavourzyme as shown by the presence of lower molecular weight peptides in the former. LPI hydrolysates prepared using Alcalase showed high ACE inhibitory activities with IC50 values ranging from 0.10 to 0.21 mg/ml. The results suggested that the globulin fraction was the main contributor towards the ACE inhibitory activity observed in lupin protein. |
| first_indexed | 2025-11-15T12:24:19Z |
| format | Article |
| id | upm-81511 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:24:19Z |
| publishDate | 2019 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-815112020-10-30T20:56:14Z http://psasir.upm.edu.my/id/eprint/81511/ Nutritional composition and angiotensin converting enzyme inhibitory activity of blue lupin (Lupinus angustifolius) Chin, Ying Yee Chew, Lye Yee Toh, Gaik Theng Salampess, Junus Azlan, Azrina Ismail, Amin AMIN BIN ISMAIL/ Chew Lye Yee// CHIN YING YEE,AZRINA BINTI AZLAN/// Gaik Theng Toh,Junus Salam Pessy The nutritional, protein and amino acid compositions of blue lupin (Lupinus angustifolius) flour were studied. The angiotensin converting enzyme (ACE) inhibitory activity of lupin protein isolate (LPI) hydrolysates prepared using Alcalase and Flavourzyme, and the Osborne protein fractions hydrolysates prepared using Alcalase were determined. Lupin flour was high in protein (43 g/100 g) and dietary fiber (34 g/100 g) but low in carbohydrate (4.8 g/100 g) and ash (3.4 g/100 g). Results from a sequential Osborne extraction procedure showed that lupin protein was comprised of 56% globulin, 26% albumin and 19% glutelin, while prolamin was only found in trace amounts. Lupin protein was high in Lys but limiting in Met. SDS-PAGE analysis suggested that protein hydrolysis catalyzed using Alcalase was more effective than Flavourzyme as shown by the presence of lower molecular weight peptides in the former. LPI hydrolysates prepared using Alcalase showed high ACE inhibitory activities with IC50 values ranging from 0.10 to 0.21 mg/ml. The results suggested that the globulin fraction was the main contributor towards the ACE inhibitory activity observed in lupin protein. Elsevier 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81511/1/Nutritional%20composition%20and%20angiotensin%20converting%20enzyme%20inhibitory%20activity%20of%20blue%20lupin%20%28Lupinus%20angustifolius%29.pdf Chin, Ying Yee and Chew, Lye Yee and Toh, Gaik Theng and Salampess, Junus and Azlan, Azrina and Ismail, Amin (2019) Nutritional composition and angiotensin converting enzyme inhibitory activity of blue lupin (Lupinus angustifolius). Food Bioscience, 31. art. no. 100401. pp. 1-10. ISSN 2212-4292 https://www.sciencedirect.com/science/article/abs/pii/S221242921730487X?casa_token=zrK1PN0WkY8AAAAA:H_P7Hjz2gpaZMHvJEzY-ggJo0FnDULeFPrtuJ6WP6RkReMrotB_y96zkZS5NWye6H0STaOJ-0y4 10.1016/j.fbio.2019.04.002 |
| spellingShingle | Chin, Ying Yee Chew, Lye Yee Toh, Gaik Theng Salampess, Junus Azlan, Azrina Ismail, Amin Nutritional composition and angiotensin converting enzyme inhibitory activity of blue lupin (Lupinus angustifolius) |
| title | Nutritional composition and angiotensin converting enzyme inhibitory activity of blue lupin (Lupinus angustifolius) |
| title_full | Nutritional composition and angiotensin converting enzyme inhibitory activity of blue lupin (Lupinus angustifolius) |
| title_fullStr | Nutritional composition and angiotensin converting enzyme inhibitory activity of blue lupin (Lupinus angustifolius) |
| title_full_unstemmed | Nutritional composition and angiotensin converting enzyme inhibitory activity of blue lupin (Lupinus angustifolius) |
| title_short | Nutritional composition and angiotensin converting enzyme inhibitory activity of blue lupin (Lupinus angustifolius) |
| title_sort | nutritional composition and angiotensin converting enzyme inhibitory activity of blue lupin (lupinus angustifolius) |
| url | http://psasir.upm.edu.my/id/eprint/81511/ http://psasir.upm.edu.my/id/eprint/81511/ http://psasir.upm.edu.my/id/eprint/81511/ http://psasir.upm.edu.my/id/eprint/81511/1/Nutritional%20composition%20and%20angiotensin%20converting%20enzyme%20inhibitory%20activity%20of%20blue%20lupin%20%28Lupinus%20angustifolius%29.pdf |