Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens
Fowl adenovirus (FAdV) is the causative agent of inclusion body hepatitis (IBH) in chickens with significant economic losses due to high mortality and poor production. It was objectives of the study to attenuate and determine the molecular characteristic of FAdV isolate (UPM1137) of Malaysia passage...
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| Format: | Article |
| Language: | English |
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Public Library of Science
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81403/ http://psasir.upm.edu.my/id/eprint/81403/1/FOWL.pdf |
| _version_ | 1848859096118198272 |
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| author | Mohamed Sohaimi, Norfitriah Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini Mat Isa, Nurulfiza |
| author_facet | Mohamed Sohaimi, Norfitriah Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini Mat Isa, Nurulfiza |
| author_sort | Mohamed Sohaimi, Norfitriah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Fowl adenovirus (FAdV) is the causative agent of inclusion body hepatitis (IBH) in chickens with significant economic losses due to high mortality and poor production. It was objectives of the study to attenuate and determine the molecular characteristic of FAdV isolate (UPM1137) of Malaysia passages in primary chicken embryo liver (CEL) cells. The cytopathic effect (CPE) was recorded and the present of the virus was detected by polymerase chain reaction (PCR). Nucleotide and amino acid changes were determined and a phylogenetic tree was constructed. The pathogenicity and immunogenicity of the virus at passage 35 (CEL35) with virus titre of 106.7TCID50/mL was determined in day old specific pathogen free (SPF) chicks via oral or subcutaneous route of inoculation. The study demonstrated that the FAdV isolate was successfully propagated and attenuated in CEL cells up to 35th consecutive passages (CEL35) with delayed of CPE formation within 48 to 72 post inoculation (pi) from CEL20 onwards. The virus caused typical CPE with basophilic intranuclear inclusion bodies, refractile and clumping of cells. The virus is belong to serotype 8b with substitution of amino acid at position 44, 133 and 185 in L1 loop of hexon gene and in knob of fiber gene at position 348 and 360 at CEL35. It is non-pathogenic, but immunogenic in SPF chickens. It was concluded that the FAdV isolate was successfully attenuated in CEL cells with molecular changes in major capsid proteins which affect its infectivity in cell culture and SPF chickens. |
| first_indexed | 2025-11-15T12:23:54Z |
| format | Article |
| id | upm-81403 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:23:54Z |
| publishDate | 2019 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-814032021-06-13T23:08:54Z http://psasir.upm.edu.my/id/eprint/81403/ Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens Mohamed Sohaimi, Norfitriah Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini Mat Isa, Nurulfiza Fowl adenovirus (FAdV) is the causative agent of inclusion body hepatitis (IBH) in chickens with significant economic losses due to high mortality and poor production. It was objectives of the study to attenuate and determine the molecular characteristic of FAdV isolate (UPM1137) of Malaysia passages in primary chicken embryo liver (CEL) cells. The cytopathic effect (CPE) was recorded and the present of the virus was detected by polymerase chain reaction (PCR). Nucleotide and amino acid changes were determined and a phylogenetic tree was constructed. The pathogenicity and immunogenicity of the virus at passage 35 (CEL35) with virus titre of 106.7TCID50/mL was determined in day old specific pathogen free (SPF) chicks via oral or subcutaneous route of inoculation. The study demonstrated that the FAdV isolate was successfully propagated and attenuated in CEL cells up to 35th consecutive passages (CEL35) with delayed of CPE formation within 48 to 72 post inoculation (pi) from CEL20 onwards. The virus caused typical CPE with basophilic intranuclear inclusion bodies, refractile and clumping of cells. The virus is belong to serotype 8b with substitution of amino acid at position 44, 133 and 185 in L1 loop of hexon gene and in knob of fiber gene at position 348 and 360 at CEL35. It is non-pathogenic, but immunogenic in SPF chickens. It was concluded that the FAdV isolate was successfully attenuated in CEL cells with molecular changes in major capsid proteins which affect its infectivity in cell culture and SPF chickens. Public Library of Science 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81403/1/FOWL.pdf Mohamed Sohaimi, Norfitriah and Bejo, Mohd Hair and Omar, Abdul Rahman and Ideris, Aini and Mat Isa, Nurulfiza (2019) Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens. PLoS One, 14 (12). pp. 1-16. ISSN 1932-6203 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0225863 10.1371/journal.pone.0225863 |
| spellingShingle | Mohamed Sohaimi, Norfitriah Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini Mat Isa, Nurulfiza Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens |
| title | Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens |
| title_full | Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens |
| title_fullStr | Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens |
| title_full_unstemmed | Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens |
| title_short | Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens |
| title_sort | molecular characterization of fowl adenovirus isolate of malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens |
| url | http://psasir.upm.edu.my/id/eprint/81403/ http://psasir.upm.edu.my/id/eprint/81403/ http://psasir.upm.edu.my/id/eprint/81403/ http://psasir.upm.edu.my/id/eprint/81403/1/FOWL.pdf |