Mast cell stabilizing effect of a geranyl acetophenone in dengue virus infection using in vitro model of DENV3-induced RBL-2H3 cells
Mast cells (MCs), a type of immune effector cell, have recently become recognized for their ability to cause vascular leakage during dengue virus (DENV) infection. Although MC stabilizers have been reported to attenuate DENV induced infection in animal studies, there are limited in vitro studies on...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Portland Press
2019
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/81291/ http://psasir.upm.edu.my/id/eprint/81291/1/CELL.pdf |
| _version_ | 1848859069120512000 |
|---|---|
| author | Tan, Ji Wei Wan Zahidi, Nuha Fahimah Siew, Audrey Foong Kow Soo, Kuan Meng Shaari, Khozirah Ahmad Israf, Daud Chee, Hui Yee Tham, Chau Ling |
| author_facet | Tan, Ji Wei Wan Zahidi, Nuha Fahimah Siew, Audrey Foong Kow Soo, Kuan Meng Shaari, Khozirah Ahmad Israf, Daud Chee, Hui Yee Tham, Chau Ling |
| author_sort | Tan, Ji Wei |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Mast cells (MCs), a type of immune effector cell, have recently become recognized for their ability to cause vascular leakage during dengue virus (DENV) infection. Although MC stabilizers have been reported to attenuate DENV induced infection in animal studies, there are limited in vitro studies on the use of MC stabilizers against DENV induced MC degranulation. 2,4,6-trihydroxy-3-geranyl acetophenone (tHGA) has been reported to be a potential MC stabilizer by inhibiting IgE-mediated MC activation in both cellular and animal models. The present study aims to establish an in vitro model of DENV3-induced RBL-2H3 cells using ketotifen fumarate as a control drug, as well as to determine the effect of tHGA on the release of MC mediators upon DENV infection. Our results demonstrated that the optimal multiplicities of infection (MOI) were 0.4 × 10-2 and 0.8 × 10-2 focus forming units (FFU)/cell. Ketotifen fumarate was proven to attenuate DENV3-induced RBL-2H3 cells degranulation in this in vitro model. In contrast, tHGA was unable to attenuate the release of both β-hexosaminidase and tumor necrosis factor (TNF)-α. Nonetheless, our study has successfully established an in vitro model of DENV3-induced RBL-2H3 cells, which might be useful for the screening of potential MC stabilizers for anti-dengue therapies. |
| first_indexed | 2025-11-15T12:23:28Z |
| format | Article |
| id | upm-81291 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:23:28Z |
| publishDate | 2019 |
| publisher | Portland Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-812912021-06-15T10:16:29Z http://psasir.upm.edu.my/id/eprint/81291/ Mast cell stabilizing effect of a geranyl acetophenone in dengue virus infection using in vitro model of DENV3-induced RBL-2H3 cells Tan, Ji Wei Wan Zahidi, Nuha Fahimah Siew, Audrey Foong Kow Soo, Kuan Meng Shaari, Khozirah Ahmad Israf, Daud Chee, Hui Yee Tham, Chau Ling Mast cells (MCs), a type of immune effector cell, have recently become recognized for their ability to cause vascular leakage during dengue virus (DENV) infection. Although MC stabilizers have been reported to attenuate DENV induced infection in animal studies, there are limited in vitro studies on the use of MC stabilizers against DENV induced MC degranulation. 2,4,6-trihydroxy-3-geranyl acetophenone (tHGA) has been reported to be a potential MC stabilizer by inhibiting IgE-mediated MC activation in both cellular and animal models. The present study aims to establish an in vitro model of DENV3-induced RBL-2H3 cells using ketotifen fumarate as a control drug, as well as to determine the effect of tHGA on the release of MC mediators upon DENV infection. Our results demonstrated that the optimal multiplicities of infection (MOI) were 0.4 × 10-2 and 0.8 × 10-2 focus forming units (FFU)/cell. Ketotifen fumarate was proven to attenuate DENV3-induced RBL-2H3 cells degranulation in this in vitro model. In contrast, tHGA was unable to attenuate the release of both β-hexosaminidase and tumor necrosis factor (TNF)-α. Nonetheless, our study has successfully established an in vitro model of DENV3-induced RBL-2H3 cells, which might be useful for the screening of potential MC stabilizers for anti-dengue therapies. Portland Press 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81291/1/CELL.pdf Tan, Ji Wei and Wan Zahidi, Nuha Fahimah and Siew, Audrey Foong Kow and Soo, Kuan Meng and Shaari, Khozirah and Ahmad Israf, Daud and Chee, Hui Yee and Tham, Chau Ling (2019) Mast cell stabilizing effect of a geranyl acetophenone in dengue virus infection using in vitro model of DENV3-induced RBL-2H3 cells. Bioscience Reports, 39 (6). pp. 1-10. ISSN 0144-8463; ESSN: 1573-4935 https://pubmed.ncbi.nlm.nih.gov/31110077/ 10.1042/BSR20181273 |
| spellingShingle | Tan, Ji Wei Wan Zahidi, Nuha Fahimah Siew, Audrey Foong Kow Soo, Kuan Meng Shaari, Khozirah Ahmad Israf, Daud Chee, Hui Yee Tham, Chau Ling Mast cell stabilizing effect of a geranyl acetophenone in dengue virus infection using in vitro model of DENV3-induced RBL-2H3 cells |
| title | Mast cell stabilizing effect of a geranyl acetophenone in dengue virus infection using in vitro model of DENV3-induced RBL-2H3 cells |
| title_full | Mast cell stabilizing effect of a geranyl acetophenone in dengue virus infection using in vitro model of DENV3-induced RBL-2H3 cells |
| title_fullStr | Mast cell stabilizing effect of a geranyl acetophenone in dengue virus infection using in vitro model of DENV3-induced RBL-2H3 cells |
| title_full_unstemmed | Mast cell stabilizing effect of a geranyl acetophenone in dengue virus infection using in vitro model of DENV3-induced RBL-2H3 cells |
| title_short | Mast cell stabilizing effect of a geranyl acetophenone in dengue virus infection using in vitro model of DENV3-induced RBL-2H3 cells |
| title_sort | mast cell stabilizing effect of a geranyl acetophenone in dengue virus infection using in vitro model of denv3-induced rbl-2h3 cells |
| url | http://psasir.upm.edu.my/id/eprint/81291/ http://psasir.upm.edu.my/id/eprint/81291/ http://psasir.upm.edu.my/id/eprint/81291/ http://psasir.upm.edu.my/id/eprint/81291/1/CELL.pdf |