Lipase-catalyzed synthesis of red pitaya (Hylocereus polyrhizus) seed oil esters for cosmeceutical applications: process optimization using response surface methodology

Esters were synthesized via the alcoholysis of red pitaya seed oil with oleyl alcohol catalyzed by immobilized lipase, Lipozyme RM IM. The effects of synthesis parameters, including temperature, time, substrate molar ratio and enzyme loading, on the yield and productivity of esters were assessed usi...

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Main Authors: Abdullah, Asiah, Abd Gani, Siti Salwa, Yap, Taufiq Yun Hin, Abdul Haiyee, Zaibunnisa, Zaidan, Uswatun Hasanah, Kassim, Mohd Azlan, Halmi, Mohd Izuan Effendi
Format: Article
Language:English
Published: Royal Society of Chemistry 2019
Online Access:http://psasir.upm.edu.my/id/eprint/81236/
http://psasir.upm.edu.my/id/eprint/81236/1/PITAYA.pdf
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author Abdullah, Asiah
Abd Gani, Siti Salwa
Yap, Taufiq Yun Hin
Abdul Haiyee, Zaibunnisa
Zaidan, Uswatun Hasanah
Kassim, Mohd Azlan
Halmi, Mohd Izuan Effendi
author_facet Abdullah, Asiah
Abd Gani, Siti Salwa
Yap, Taufiq Yun Hin
Abdul Haiyee, Zaibunnisa
Zaidan, Uswatun Hasanah
Kassim, Mohd Azlan
Halmi, Mohd Izuan Effendi
author_sort Abdullah, Asiah
building UPM Institutional Repository
collection Online Access
description Esters were synthesized via the alcoholysis of red pitaya seed oil with oleyl alcohol catalyzed by immobilized lipase, Lipozyme RM IM. The effects of synthesis parameters, including temperature, time, substrate molar ratio and enzyme loading, on the yield and productivity of esters were assessed using a central composite response surface design. The optimum yield and productivity were predicted to be about 80.00% and 0.58 mmol h−1, respectively, at a synthesis temperature of 50.5 °C, time of 4 h, substrate molar ratio of 3.4 : 1 and with 0.17 g of enzyme. Esters were synthesized under the optimum synthesis conditions; it was found that the average yield and productivity were 82.48 ± 4.57% and 0.62 ± 0.04 mmol h−1, respectively, revealing good correspondence with the predicted values. The main esters were oleyl linoleate, oleyl oleate, oleyl palmitate and oleyl stearate. The synthesized esters exhibited no irritancy effects and their physicochemical properties showed their suitability for use as cosmeceutical ingredients.
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spelling upm-812362021-06-15T23:56:48Z http://psasir.upm.edu.my/id/eprint/81236/ Lipase-catalyzed synthesis of red pitaya (Hylocereus polyrhizus) seed oil esters for cosmeceutical applications: process optimization using response surface methodology Abdullah, Asiah Abd Gani, Siti Salwa Yap, Taufiq Yun Hin Abdul Haiyee, Zaibunnisa Zaidan, Uswatun Hasanah Kassim, Mohd Azlan Halmi, Mohd Izuan Effendi Esters were synthesized via the alcoholysis of red pitaya seed oil with oleyl alcohol catalyzed by immobilized lipase, Lipozyme RM IM. The effects of synthesis parameters, including temperature, time, substrate molar ratio and enzyme loading, on the yield and productivity of esters were assessed using a central composite response surface design. The optimum yield and productivity were predicted to be about 80.00% and 0.58 mmol h−1, respectively, at a synthesis temperature of 50.5 °C, time of 4 h, substrate molar ratio of 3.4 : 1 and with 0.17 g of enzyme. Esters were synthesized under the optimum synthesis conditions; it was found that the average yield and productivity were 82.48 ± 4.57% and 0.62 ± 0.04 mmol h−1, respectively, revealing good correspondence with the predicted values. The main esters were oleyl linoleate, oleyl oleate, oleyl palmitate and oleyl stearate. The synthesized esters exhibited no irritancy effects and their physicochemical properties showed their suitability for use as cosmeceutical ingredients. Royal Society of Chemistry 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81236/1/PITAYA.pdf Abdullah, Asiah and Abd Gani, Siti Salwa and Yap, Taufiq Yun Hin and Abdul Haiyee, Zaibunnisa and Zaidan, Uswatun Hasanah and Kassim, Mohd Azlan and Halmi, Mohd Izuan Effendi (2019) Lipase-catalyzed synthesis of red pitaya (Hylocereus polyrhizus) seed oil esters for cosmeceutical applications: process optimization using response surface methodology. RSC Advances, 9 (10). pp. 5599-5609. ISSN 2046-2069 https://pubs.rsc.org/en/content/articlelanding/2019/ra/c8ra09418g#!divAbstract 10.1039/c8ra09418g
spellingShingle Abdullah, Asiah
Abd Gani, Siti Salwa
Yap, Taufiq Yun Hin
Abdul Haiyee, Zaibunnisa
Zaidan, Uswatun Hasanah
Kassim, Mohd Azlan
Halmi, Mohd Izuan Effendi
Lipase-catalyzed synthesis of red pitaya (Hylocereus polyrhizus) seed oil esters for cosmeceutical applications: process optimization using response surface methodology
title Lipase-catalyzed synthesis of red pitaya (Hylocereus polyrhizus) seed oil esters for cosmeceutical applications: process optimization using response surface methodology
title_full Lipase-catalyzed synthesis of red pitaya (Hylocereus polyrhizus) seed oil esters for cosmeceutical applications: process optimization using response surface methodology
title_fullStr Lipase-catalyzed synthesis of red pitaya (Hylocereus polyrhizus) seed oil esters for cosmeceutical applications: process optimization using response surface methodology
title_full_unstemmed Lipase-catalyzed synthesis of red pitaya (Hylocereus polyrhizus) seed oil esters for cosmeceutical applications: process optimization using response surface methodology
title_short Lipase-catalyzed synthesis of red pitaya (Hylocereus polyrhizus) seed oil esters for cosmeceutical applications: process optimization using response surface methodology
title_sort lipase-catalyzed synthesis of red pitaya (hylocereus polyrhizus) seed oil esters for cosmeceutical applications: process optimization using response surface methodology
url http://psasir.upm.edu.my/id/eprint/81236/
http://psasir.upm.edu.my/id/eprint/81236/
http://psasir.upm.edu.my/id/eprint/81236/
http://psasir.upm.edu.my/id/eprint/81236/1/PITAYA.pdf