Development of an efficient particle bombardment transformation system for the endemic orchid, Phalaenopsis bellina
Phalaenopsis bellina is an important indigenous orchid with high commercial value. In this study, we established an efficient particle bombardment transformation system for P. bellina using the protocorm-like-bodies (PLBs) as target tissues. Leaf derived PLBs were proliferated on ½ strength Murashig...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81147/ http://psasir.upm.edu.my/id/eprint/81147/1/ENDEMIC%20ORCHID.pdf |
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| author | Chew, Yee Chern Wan Abdullah, Wan Muhamad Asrul Nizam Kok, Andrew De-Xian Abdullah, Janna Ong Mahmood, Maziah Lai, Kok Song |
| author_facet | Chew, Yee Chern Wan Abdullah, Wan Muhamad Asrul Nizam Kok, Andrew De-Xian Abdullah, Janna Ong Mahmood, Maziah Lai, Kok Song |
| author_sort | Chew, Yee Chern |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Phalaenopsis bellina is an important indigenous orchid with high commercial value. In this study, we established an efficient particle bombardment transformation system for P. bellina using the protocorm-like-bodies (PLBs) as target tissues. Leaf derived PLBs were proliferated on ½ strength Murashige and Skoog (MS) medium supplemented with 0.8 µM 2,4 dichlorophenoxyacetic acid (2, 4-D). Both physical and biological parameters affecting the transformation system were optimised using the green-fluorescent protein (GFP) and β-glucuronidase (GUS) as reporter systems. Optimal bombardment conditions with 6 cm target tissues distance, 1100 psi acceleration pressure, 1.0 μm gold particle size, 27mmHg chamber vacuum pressure, single bombardment time, spermidine as DNA precipitation agent, 72 h post bombardment incubation time, 2 μg plasmid DNA in 0.15:0.12 pmol ratio (pSMCHS:p35SGFP) were successfully determined. Surviving PLBs transformants were successfully recovered from the hygromycin selection medium and verified using genomic PCR analysis. The established system is not only useful for a simple and reliable transient gene analysis but as well as generating stable transformants for selective traits improvement in orchids. |
| first_indexed | 2025-11-15T12:22:57Z |
| format | Article |
| id | upm-81147 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:22:57Z |
| publishDate | 2019 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-811472020-09-09T07:10:11Z http://psasir.upm.edu.my/id/eprint/81147/ Development of an efficient particle bombardment transformation system for the endemic orchid, Phalaenopsis bellina Chew, Yee Chern Wan Abdullah, Wan Muhamad Asrul Nizam Kok, Andrew De-Xian Abdullah, Janna Ong Mahmood, Maziah Lai, Kok Song Phalaenopsis bellina is an important indigenous orchid with high commercial value. In this study, we established an efficient particle bombardment transformation system for P. bellina using the protocorm-like-bodies (PLBs) as target tissues. Leaf derived PLBs were proliferated on ½ strength Murashige and Skoog (MS) medium supplemented with 0.8 µM 2,4 dichlorophenoxyacetic acid (2, 4-D). Both physical and biological parameters affecting the transformation system were optimised using the green-fluorescent protein (GFP) and β-glucuronidase (GUS) as reporter systems. Optimal bombardment conditions with 6 cm target tissues distance, 1100 psi acceleration pressure, 1.0 μm gold particle size, 27mmHg chamber vacuum pressure, single bombardment time, spermidine as DNA precipitation agent, 72 h post bombardment incubation time, 2 μg plasmid DNA in 0.15:0.12 pmol ratio (pSMCHS:p35SGFP) were successfully determined. Surviving PLBs transformants were successfully recovered from the hygromycin selection medium and verified using genomic PCR analysis. The established system is not only useful for a simple and reliable transient gene analysis but as well as generating stable transformants for selective traits improvement in orchids. Penerbit Universiti Kebangsaan Malaysia 2019 Article NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/81147/1/ENDEMIC%20ORCHID.pdf Chew, Yee Chern and Wan Abdullah, Wan Muhamad Asrul Nizam and Kok, Andrew De-Xian and Abdullah, Janna Ong and Mahmood, Maziah and Lai, Kok Song (2019) Development of an efficient particle bombardment transformation system for the endemic orchid, Phalaenopsis bellina. Sains Malaysiana, 48 (9). pp. 1867-1877. ISSN 0126-6039 http://www.ukm.my/jsm/pdf_files/SM-PDF-48-9-2019/07%20Yee-Chern%20Chew.pdf 10.17576/jsm-2019-4809-07 |
| spellingShingle | Chew, Yee Chern Wan Abdullah, Wan Muhamad Asrul Nizam Kok, Andrew De-Xian Abdullah, Janna Ong Mahmood, Maziah Lai, Kok Song Development of an efficient particle bombardment transformation system for the endemic orchid, Phalaenopsis bellina |
| title | Development of an efficient particle bombardment transformation system for the endemic orchid, Phalaenopsis bellina |
| title_full | Development of an efficient particle bombardment transformation system for the endemic orchid, Phalaenopsis bellina |
| title_fullStr | Development of an efficient particle bombardment transformation system for the endemic orchid, Phalaenopsis bellina |
| title_full_unstemmed | Development of an efficient particle bombardment transformation system for the endemic orchid, Phalaenopsis bellina |
| title_short | Development of an efficient particle bombardment transformation system for the endemic orchid, Phalaenopsis bellina |
| title_sort | development of an efficient particle bombardment transformation system for the endemic orchid, phalaenopsis bellina |
| url | http://psasir.upm.edu.my/id/eprint/81147/ http://psasir.upm.edu.my/id/eprint/81147/ http://psasir.upm.edu.my/id/eprint/81147/ http://psasir.upm.edu.my/id/eprint/81147/1/ENDEMIC%20ORCHID.pdf |