Determination of cell viability using acridine orange/propidium iodide dual-spectrofluorometry assay
In vitro cell viability tests are usually done using 3-(4,5 dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) method. A new spectrofluorometry method was developed using acridine orange (AO) and propidium iodide (PI) using multi-label microplate reader. Nine bio...
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81120/ http://psasir.upm.edu.my/id/eprint/81120/1/CELL.pdf |
| _version_ | 1848859028698955776 |
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| author | Hussain, Husniza Santhana, Raj L. Ahmad, Syahida Abd Razak, Mohd Fuat Wan Mohamud, Wan Nazaimoon Bakar, Jamilah Mohd. Ghazali, Hasanah Yildiz, Fatih |
| author_facet | Hussain, Husniza Santhana, Raj L. Ahmad, Syahida Abd Razak, Mohd Fuat Wan Mohamud, Wan Nazaimoon Bakar, Jamilah Mohd. Ghazali, Hasanah Yildiz, Fatih |
| author_sort | Hussain, Husniza |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In vitro cell viability tests are usually done using 3-(4,5 dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) method. A new spectrofluorometry method was developed using acridine orange (AO) and propidium iodide (PI) using multi-label microplate reader. Nine biogenic amines (BAs) [histamine (HIM), putrescine (PUT), cadaverine (CAD), 2-phenylethylamine (PHM), tyramine (TYM), tryptamine (TPM), spermine (SPM), spermidine (SPD) and agmatine (AGM)] were exposed to RAW 264.7 macrophage in singles at 37°C with 5% carbon dioxide supplementation for 18–24 hours and cell viability was determined using MTS method and AO/PI developed method using dual-spectrofluorometry. Based on MTS assay, SPM and SPD were found to be cytotoxic and it was supported by AO/PI assay. The precedence of disintegration in the nucleus rather than mitochondria upon cell non-viability was also supported by transmission electron microscopy (TEM). The results showed that AO/PI method could be used as an alternative method to determine cytotoxicity besides usual usage in confocal microscopy. |
| first_indexed | 2025-11-15T12:22:50Z |
| format | Article |
| id | upm-81120 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:22:50Z |
| publishDate | 2019 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-811202020-09-10T03:11:20Z http://psasir.upm.edu.my/id/eprint/81120/ Determination of cell viability using acridine orange/propidium iodide dual-spectrofluorometry assay Hussain, Husniza Santhana, Raj L. Ahmad, Syahida Abd Razak, Mohd Fuat Wan Mohamud, Wan Nazaimoon Bakar, Jamilah Mohd. Ghazali, Hasanah Yildiz, Fatih In vitro cell viability tests are usually done using 3-(4,5 dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) method. A new spectrofluorometry method was developed using acridine orange (AO) and propidium iodide (PI) using multi-label microplate reader. Nine biogenic amines (BAs) [histamine (HIM), putrescine (PUT), cadaverine (CAD), 2-phenylethylamine (PHM), tyramine (TYM), tryptamine (TPM), spermine (SPM), spermidine (SPD) and agmatine (AGM)] were exposed to RAW 264.7 macrophage in singles at 37°C with 5% carbon dioxide supplementation for 18–24 hours and cell viability was determined using MTS method and AO/PI developed method using dual-spectrofluorometry. Based on MTS assay, SPM and SPD were found to be cytotoxic and it was supported by AO/PI assay. The precedence of disintegration in the nucleus rather than mitochondria upon cell non-viability was also supported by transmission electron microscopy (TEM). The results showed that AO/PI method could be used as an alternative method to determine cytotoxicity besides usual usage in confocal microscopy. Taylor & Francis 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81120/1/CELL.pdf Hussain, Husniza and Santhana, Raj L. and Ahmad, Syahida and Abd Razak, Mohd Fuat and Wan Mohamud, Wan Nazaimoon and Bakar, Jamilah and Mohd. Ghazali, Hasanah and Yildiz, Fatih (2019) Determination of cell viability using acridine orange/propidium iodide dual-spectrofluorometry assay. Cogent Food & Agriculture, 5. pp. 1-9. ISSN 2331-1932 https://www.tandfonline.com/loi/oafa20 10.1080/23311932.2019.1582398 |
| spellingShingle | Hussain, Husniza Santhana, Raj L. Ahmad, Syahida Abd Razak, Mohd Fuat Wan Mohamud, Wan Nazaimoon Bakar, Jamilah Mohd. Ghazali, Hasanah Yildiz, Fatih Determination of cell viability using acridine orange/propidium iodide dual-spectrofluorometry assay |
| title | Determination of cell viability using acridine orange/propidium iodide dual-spectrofluorometry assay |
| title_full | Determination of cell viability using acridine orange/propidium iodide dual-spectrofluorometry assay |
| title_fullStr | Determination of cell viability using acridine orange/propidium iodide dual-spectrofluorometry assay |
| title_full_unstemmed | Determination of cell viability using acridine orange/propidium iodide dual-spectrofluorometry assay |
| title_short | Determination of cell viability using acridine orange/propidium iodide dual-spectrofluorometry assay |
| title_sort | determination of cell viability using acridine orange/propidium iodide dual-spectrofluorometry assay |
| url | http://psasir.upm.edu.my/id/eprint/81120/ http://psasir.upm.edu.my/id/eprint/81120/ http://psasir.upm.edu.my/id/eprint/81120/ http://psasir.upm.edu.my/id/eprint/81120/1/CELL.pdf |