Molecular characterisation of the GdhA- derivative of Pasteurella multocida B:2
Pasteurella multocida B:2 is an important veterinary pathogen causing fatal and acute haemorrhagic septicaemia (HS) in bovine. A live vaccine candidate, P. multocida B:2 GDH7 was reported to enable protection in cattle and buffaloes via intranasal (i. n.) administration. This potential vaccine was a...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81076/ http://psasir.upm.edu.my/id/eprint/81076/1/VACCINE.pdf |
| _version_ | 1848859017609216000 |
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| author | Muhammad Azam, Farahani Saad, Mohd Zamri Abdul Rahim, Raha Chumnanpuen, Pramote E-kobon, Teerasak Othman, Sarah |
| author_facet | Muhammad Azam, Farahani Saad, Mohd Zamri Abdul Rahim, Raha Chumnanpuen, Pramote E-kobon, Teerasak Othman, Sarah |
| author_sort | Muhammad Azam, Farahani |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pasteurella multocida B:2 is an important veterinary pathogen causing fatal and acute haemorrhagic septicaemia (HS) in bovine. A live vaccine candidate, P. multocida B:2 GDH7 was reported to enable protection in cattle and buffaloes via intranasal (i. n.) administration. This potential vaccine was also reported to be self-transmitted from the vaccinated animal to the free-ranging animals allowing wider vaccination coverage. Prior to commercialisation, this potential vaccine requires further characterisation in accordance with the authoritative guidelines from the World Organisation for Animal Health (OIE). Hence, in this study, the potential vaccine strain, P. multocida B:2 GDH7 and the virulent parent strain were characterised through genomic and proteomic profiling. A crucial first step was to develop a sensitive yet simple and robust identification test to differentiate both strains which has been achieved by the development of a precise yet straightforward PCR method. In genomic profiling, Repetitive Extragenic Palindromic sequence-PCR (REP-PCR) was manipulated and both strains have a different display of genomic DNA band patterns. Some of the major OMPs were observed and prominent immunogens of P. multocida, OmpA and OmpH were observed to be expressed differently between these strains through SDS-PAGE analysis. In conclusion, a reproducible PCR detection method has enabled differentiation of both strains. Further characterisation of these strains shows a significantly different profile through genomic and proteomic profiling. |
| first_indexed | 2025-11-15T12:22:39Z |
| format | Article |
| id | upm-81076 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:22:39Z |
| publishDate | 2021 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-810762021-07-21T20:29:27Z http://psasir.upm.edu.my/id/eprint/81076/ Molecular characterisation of the GdhA- derivative of Pasteurella multocida B:2 Muhammad Azam, Farahani Saad, Mohd Zamri Abdul Rahim, Raha Chumnanpuen, Pramote E-kobon, Teerasak Othman, Sarah Pasteurella multocida B:2 is an important veterinary pathogen causing fatal and acute haemorrhagic septicaemia (HS) in bovine. A live vaccine candidate, P. multocida B:2 GDH7 was reported to enable protection in cattle and buffaloes via intranasal (i. n.) administration. This potential vaccine was also reported to be self-transmitted from the vaccinated animal to the free-ranging animals allowing wider vaccination coverage. Prior to commercialisation, this potential vaccine requires further characterisation in accordance with the authoritative guidelines from the World Organisation for Animal Health (OIE). Hence, in this study, the potential vaccine strain, P. multocida B:2 GDH7 and the virulent parent strain were characterised through genomic and proteomic profiling. A crucial first step was to develop a sensitive yet simple and robust identification test to differentiate both strains which has been achieved by the development of a precise yet straightforward PCR method. In genomic profiling, Repetitive Extragenic Palindromic sequence-PCR (REP-PCR) was manipulated and both strains have a different display of genomic DNA band patterns. Some of the major OMPs were observed and prominent immunogens of P. multocida, OmpA and OmpH were observed to be expressed differently between these strains through SDS-PAGE analysis. In conclusion, a reproducible PCR detection method has enabled differentiation of both strains. Further characterisation of these strains shows a significantly different profile through genomic and proteomic profiling. Universiti Putra Malaysia Press 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81076/1/VACCINE.pdf Muhammad Azam, Farahani and Saad, Mohd Zamri and Abdul Rahim, Raha and Chumnanpuen, Pramote and E-kobon, Teerasak and Othman, Sarah (2021) Molecular characterisation of the GdhA- derivative of Pasteurella multocida B:2. Pertanika Journal of Tropical Agricultural Science, 44 (1). pp. 171-192. ISSN 0128-7680; ESSN: 2231-8526 http://www.pertanika.upm.edu.my/pjtas/browse/regular-issue?article=JTAS-2125-2020 10.47836/pjtas.44.1.10 |
| spellingShingle | Muhammad Azam, Farahani Saad, Mohd Zamri Abdul Rahim, Raha Chumnanpuen, Pramote E-kobon, Teerasak Othman, Sarah Molecular characterisation of the GdhA- derivative of Pasteurella multocida B:2 |
| title | Molecular characterisation of the GdhA- derivative of Pasteurella multocida B:2 |
| title_full | Molecular characterisation of the GdhA- derivative of Pasteurella multocida B:2 |
| title_fullStr | Molecular characterisation of the GdhA- derivative of Pasteurella multocida B:2 |
| title_full_unstemmed | Molecular characterisation of the GdhA- derivative of Pasteurella multocida B:2 |
| title_short | Molecular characterisation of the GdhA- derivative of Pasteurella multocida B:2 |
| title_sort | molecular characterisation of the gdha- derivative of pasteurella multocida b:2 |
| url | http://psasir.upm.edu.my/id/eprint/81076/ http://psasir.upm.edu.my/id/eprint/81076/ http://psasir.upm.edu.my/id/eprint/81076/ http://psasir.upm.edu.my/id/eprint/81076/1/VACCINE.pdf |