Comparison of antioxidants content and activity of Nephelium mutabile rind extracted using ethanol and water

This study is aimed to determine and compare the effect of different solvent polarities on total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities using water and ratio of aqueous/ethanol (40, 60, and 80%) for the extraction of Nephelium mutabile rind. The TPC and TFC...

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Main Authors: Mohamed Sopee, Muhammad Syafiq, Azlan, Azrina, Khoo, Hock Eng
Format: Article
Language:English
Published: Springer 2019
Online Access:http://psasir.upm.edu.my/id/eprint/80979/
http://psasir.upm.edu.my/id/eprint/80979/1/DPPH.pdf
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author Mohamed Sopee, Muhammad Syafiq
Azlan, Azrina
Khoo, Hock Eng
author_facet Mohamed Sopee, Muhammad Syafiq
Azlan, Azrina
Khoo, Hock Eng
author_sort Mohamed Sopee, Muhammad Syafiq
building UPM Institutional Repository
collection Online Access
description This study is aimed to determine and compare the effect of different solvent polarities on total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities using water and ratio of aqueous/ethanol (40, 60, and 80%) for the extraction of Nephelium mutabile rind. The TPC and TFC were evaluated using Folin–Ciocalteu reagent and aluminum chloride colorimetric method, respectively; while antioxidant activities were determined by DPPH radical scavenging and FRAP assays. The highest TPC was determined in the water extract of N. mutabile rind, followed by 80% ethanol extract. The highest TFC was found in 60% ethanol extract of N. mutabile rind, followed by 80% ethanol extract. Similar to the TPC, the N. mutabile rind extract exhibited the highest FRAP value when extracted with water, followed by 80% ethanol extract. However, the antioxidant activity assessed by DPPH radical scavenging assay was the highest in 80% ethanol extract of N. mutabile rind, followed by water extract. A strong and positive correlation (r = 0.655) was found between TPC and antioxidant activities of the N. mutabile rind extracts. The result obtained indicates that the high antioxidant activities of N. mutabile rind are probably due to the high TPC in the extract. The results from this study indicated that water could be a useful solvent in extracting antioxidant compounds from the N. mutabile rind. In future, the water and 80% ethanol extracts of N. mutabile rind could be used as nutraceuticals.
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spelling upm-809792020-10-14T20:40:41Z http://psasir.upm.edu.my/id/eprint/80979/ Comparison of antioxidants content and activity of Nephelium mutabile rind extracted using ethanol and water Mohamed Sopee, Muhammad Syafiq Azlan, Azrina Khoo, Hock Eng This study is aimed to determine and compare the effect of different solvent polarities on total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities using water and ratio of aqueous/ethanol (40, 60, and 80%) for the extraction of Nephelium mutabile rind. The TPC and TFC were evaluated using Folin–Ciocalteu reagent and aluminum chloride colorimetric method, respectively; while antioxidant activities were determined by DPPH radical scavenging and FRAP assays. The highest TPC was determined in the water extract of N. mutabile rind, followed by 80% ethanol extract. The highest TFC was found in 60% ethanol extract of N. mutabile rind, followed by 80% ethanol extract. Similar to the TPC, the N. mutabile rind extract exhibited the highest FRAP value when extracted with water, followed by 80% ethanol extract. However, the antioxidant activity assessed by DPPH radical scavenging assay was the highest in 80% ethanol extract of N. mutabile rind, followed by water extract. A strong and positive correlation (r = 0.655) was found between TPC and antioxidant activities of the N. mutabile rind extracts. The result obtained indicates that the high antioxidant activities of N. mutabile rind are probably due to the high TPC in the extract. The results from this study indicated that water could be a useful solvent in extracting antioxidant compounds from the N. mutabile rind. In future, the water and 80% ethanol extracts of N. mutabile rind could be used as nutraceuticals. Springer 2019-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80979/1/DPPH.pdf Mohamed Sopee, Muhammad Syafiq and Azlan, Azrina and Khoo, Hock Eng (2019) Comparison of antioxidants content and activity of Nephelium mutabile rind extracted using ethanol and water. Journal of Food Measurement and Characterization, 13 (3). pp. 1958-1963. ISSN 2193-4126; ESSN: 2193-4134 https://link.springer.com/article/10.1007/s11694-019-00114-7 10.1007/s11694-019-00114-7
spellingShingle Mohamed Sopee, Muhammad Syafiq
Azlan, Azrina
Khoo, Hock Eng
Comparison of antioxidants content and activity of Nephelium mutabile rind extracted using ethanol and water
title Comparison of antioxidants content and activity of Nephelium mutabile rind extracted using ethanol and water
title_full Comparison of antioxidants content and activity of Nephelium mutabile rind extracted using ethanol and water
title_fullStr Comparison of antioxidants content and activity of Nephelium mutabile rind extracted using ethanol and water
title_full_unstemmed Comparison of antioxidants content and activity of Nephelium mutabile rind extracted using ethanol and water
title_short Comparison of antioxidants content and activity of Nephelium mutabile rind extracted using ethanol and water
title_sort comparison of antioxidants content and activity of nephelium mutabile rind extracted using ethanol and water
url http://psasir.upm.edu.my/id/eprint/80979/
http://psasir.upm.edu.my/id/eprint/80979/
http://psasir.upm.edu.my/id/eprint/80979/
http://psasir.upm.edu.my/id/eprint/80979/1/DPPH.pdf