Comparative screening methods for the detection of biosurfactant-producing capability of Antarctic hydrocarbon-degrading Pseudomonas sp.
Four preliminary screening methods for biosurfactant synthesis - drop collapse assay, oil displacement activity, microplate assay and emulsification index (E24) were compared and evaluated for their reliability and ease of use. All screening methods showed positive indications for the synthesis of b...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hibiscus
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80968/ http://psasir.upm.edu.my/id/eprint/80968/1/ANTARTIC.pdf |
| _version_ | 1848858992187539456 |
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| author | Saruni, Nurul Hani Abdul Razak, Syafiqah Habib, Syahir Ahmad, Siti Aqlima Alias, Siti Aisyah Wan Johari, Wan Lutfi Smykla, Jerzy Yasid, Nur Adeela |
| author_facet | Saruni, Nurul Hani Abdul Razak, Syafiqah Habib, Syahir Ahmad, Siti Aqlima Alias, Siti Aisyah Wan Johari, Wan Lutfi Smykla, Jerzy Yasid, Nur Adeela |
| author_sort | Saruni, Nurul Hani |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Four preliminary screening methods for biosurfactant synthesis - drop collapse assay, oil displacement activity, microplate assay and emulsification index (E24) were compared and evaluated for their reliability and ease of use. All screening methods showed positive indications for the synthesis of biological surface-active agents. Nevertheless, partial collapse of the supernatant and low emulsification index (E24) of Pseudomonassp. might signify a low production of biosurfactants. Based on our observation, both drop collapse and oil displacement assay is the fastest, easiest and most reliable analytical routine to be suggested to screen for biosurfactant producing strains. In the extent for a high throughput screening (HTS), drop collapse assay is the best method for an accurate screening of biosurfactant producers. |
| first_indexed | 2025-11-15T12:22:15Z |
| format | Article |
| id | upm-80968 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:22:15Z |
| publishDate | 2019 |
| publisher | Hibiscus |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-809682020-10-14T20:30:51Z http://psasir.upm.edu.my/id/eprint/80968/ Comparative screening methods for the detection of biosurfactant-producing capability of Antarctic hydrocarbon-degrading Pseudomonas sp. Saruni, Nurul Hani Abdul Razak, Syafiqah Habib, Syahir Ahmad, Siti Aqlima Alias, Siti Aisyah Wan Johari, Wan Lutfi Smykla, Jerzy Yasid, Nur Adeela Four preliminary screening methods for biosurfactant synthesis - drop collapse assay, oil displacement activity, microplate assay and emulsification index (E24) were compared and evaluated for their reliability and ease of use. All screening methods showed positive indications for the synthesis of biological surface-active agents. Nevertheless, partial collapse of the supernatant and low emulsification index (E24) of Pseudomonassp. might signify a low production of biosurfactants. Based on our observation, both drop collapse and oil displacement assay is the fastest, easiest and most reliable analytical routine to be suggested to screen for biosurfactant producing strains. In the extent for a high throughput screening (HTS), drop collapse assay is the best method for an accurate screening of biosurfactant producers. Hibiscus 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80968/1/ANTARTIC.pdf Saruni, Nurul Hani and Abdul Razak, Syafiqah and Habib, Syahir and Ahmad, Siti Aqlima and Alias, Siti Aisyah and Wan Johari, Wan Lutfi and Smykla, Jerzy and Yasid, Nur Adeela (2019) Comparative screening methods for the detection of biosurfactant-producing capability of Antarctic hydrocarbon-degrading Pseudomonas sp. Journal of Environmental Microbiology and Toxicology, 7 (1). pp. 44-47. ISSN 2289-5906 http://journal.hibiscuspublisher.com/index.php/JEMAT/index |
| spellingShingle | Saruni, Nurul Hani Abdul Razak, Syafiqah Habib, Syahir Ahmad, Siti Aqlima Alias, Siti Aisyah Wan Johari, Wan Lutfi Smykla, Jerzy Yasid, Nur Adeela Comparative screening methods for the detection of biosurfactant-producing capability of Antarctic hydrocarbon-degrading Pseudomonas sp. |
| title | Comparative screening methods for the detection of biosurfactant-producing capability of Antarctic hydrocarbon-degrading Pseudomonas sp. |
| title_full | Comparative screening methods for the detection of biosurfactant-producing capability of Antarctic hydrocarbon-degrading Pseudomonas sp. |
| title_fullStr | Comparative screening methods for the detection of biosurfactant-producing capability of Antarctic hydrocarbon-degrading Pseudomonas sp. |
| title_full_unstemmed | Comparative screening methods for the detection of biosurfactant-producing capability of Antarctic hydrocarbon-degrading Pseudomonas sp. |
| title_short | Comparative screening methods for the detection of biosurfactant-producing capability of Antarctic hydrocarbon-degrading Pseudomonas sp. |
| title_sort | comparative screening methods for the detection of biosurfactant-producing capability of antarctic hydrocarbon-degrading pseudomonas sp. |
| url | http://psasir.upm.edu.my/id/eprint/80968/ http://psasir.upm.edu.my/id/eprint/80968/ http://psasir.upm.edu.my/id/eprint/80968/1/ANTARTIC.pdf |