Comparative analysis of current diagnostic PCR assays in detecting pathogenic Leptospira isolates from environmental samples

QR Code

Comparative analysis of current diagnostic PCR assays in detecting pathogenic Leptospira isolates from environmental samples

Objective: To compare the efficiency of routine diagnostic PCR assays in detecting pathogenic Leptospira isolated from water and soils. Methods: Seven routine assays targeting six genes (lipL32, flaB, gyrB, lfb1, secY and ligB) were evaluated and compared on the cultures of two groups of pathogenic...

Full description

Bibliographic Details
Main Authors:	Yap, May Ling, Sekawi, Zamberi, Chee, Hui Yee, Ong, Alan Han Kiat, Vasantha Kumari, Neela
Format:	Article
Language:	English
Published:	Wolters Kluwer - Medknow 2019
Online Access:	http://psasir.upm.edu.my/id/eprint/80964/ http://psasir.upm.edu.my/id/eprint/80964/1/LEPTOSPIRA.pdf

Similar Items

Evaluation of diagnostic accuracy of loop-mediated isothermal amplification method (LAMP) compared with polymerase chain reaction (PCR) for Leptospira spp. in clinical samples: a systematic review and meta-analysis
by: Gunasegar, Shan, et al.
Published: (2021)

Leptospira dzianensis and Leptospira putramalaysiae are later heterotypic synonyms of Leptospira yasudae and Leptospira stimsonii
by: Casanovas-Massana, Arnau, et al.
Published: (2021)

TaqMan real-time PCR for detection of pathogenic Leptospira spp. in canine clinical samples
by: Abdul Rahman, Mohammad Sabri, et al.
Published: (2023)

Molecular detection and characterization of pathogenic leptospira species in environmental samples of selected districts in Perak, Malaysia
by: Yap, May Ling
Published: (2019)

A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus
by: Ghaznavi-Rad, Ehsanollah, et al.
Published: (2010)

In vivo and in silico virulence analysis of Leptospira species isolated from environments and rodents in leptospirosis outbreak areas in Malaysia
by: Philip, Noraini, et al.
Published: (2021)

Long-term preservation of Leptospira spp.: challenges and prospects
by: Philip, Noraini, et al.
Published: (2018)

Microhabitat factors influenced the prevalence of pathogenic Leptospira spp. in small mammal host
by: Yusof, Muhammad Afif, et al.
Published: (2019)

Diagnostic accuracy of rapid diagnostic tests for the early detection of leptospirosis
by: Ramli, Siti Nur Alia, et al.
Published: (2019)

Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes
by: Nurul Farhana Zulkifli,, et al.
Published: (2018)

Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli
by: Al-Hetar, Khadega Yahya Abdullah, et al.
Published: (2011)

Molecular pcr assays for detection of Ganoderma pathogenic to oil palm in Malaysia
by: Nur Hailini Zainol Hilmi,, et al.
Published: (2022)

Leptospira interrogans and Leptospira kirschneri are the dominant Leptospira species causing human leptospirosis in Central Malaysia
by: Philip, Noraini, et al.
Published: (2020)

Pulmonary haemorrhage as the earliest sign of severe leptospirosis in hamster model challenged with Leptospira interrogans strain HP358
by: Philip, Noraini, et al.
Published: (2022)

Genomics data of L. borgpetersenii strain HP364 and L. weilii strain SC295 isolated from rodents captured from human leptospirosis outbreak areas in Selangor, Malaysia
by: Neela, Vasantha Kumari, et al.
Published: (2023)

Multiplex PCR assays for the detection of clinically relevant antibiotic resistance genes in Staphylococcus aureus isolated from Malaysian hospitals.
by: E., Amghalia, et al.
Published: (2009)

Isolation and identification of pathogenic leptospira spp from environmental samples during and after 2017 flood in Kelantan
by: Katman, Mohammad Salihen
Published: (2017)

Diagnostic accuracy of genetic markers and nucleic acid techniques for the detection of Leptospira in clinical samples: a meta-analysis
by: Lam, Jia Yong, et al.
Published: (2020)

Development of a diagnostic real time pcr assay for molecular detection and characterization of mycoplasma gallisepticum
by: Yasmin, Farhana
Published: (2013)

Development of a diagnostic real time pcr assay for molecular detection and characterization of mycoplasma gallisepticum
by: Yasmin, Farhana
Published: (2013)

A MassTag PCR Assay for the Syndromic Detection of Pathogens that can cause Neurological Disease
by: Williams, David, et al.
Published: (2011)

Development of a MassTag PCR Assay for the Syndromic Detection of Pathogens that can Cause Encephalitis
by: Williams, David, et al.
Published: (2009)

Development of a multiplex real-time pcr assay for detection of fungal pathogens in invasive mycoses
by: Ismadi, Yasmin Khairani Muhammad
Published: (2024)

Duplex PCR assay for simultaneous detection of two aminoglycoside resistance genes in clinical samples of enterobacteriaceae
by: Theo, Chun Hao
Published: (2022)

Detection of pathogenic Leptospira in rats and phylogenetic analysis using outer membrane lipoprotein Lipl32 gene at two major public markets
by: Wan, Steffi Julan, et al.
Published: (2022)

A versatile isothermal amplification assay for the detection of leptospires from various sample types
by: Othman, Shuhaidah, et al.
Published: (2022)

A blood agar for detecting pathogen
by: Amin Nordin, Syafinaz, et al.
Published: (2014)

Health and safety: real time detection of Bukholderia pseudomallei and pathogenic leptospira spp using a new portable amplification diagnostics system
by: Aziah, Ismail
Published: (2017)

Clinical Evaluation of a MassCode PCR Assay for the Syndromic Detection of Pathogens that cause Respiratory Disease
by: Smith, D., et al.
Published: (2011)

Towards Development of a MassTag PCR Assay for the Syndromic Detection of Pathogens that can Cause Encephalitis
by: Williams, David, et al.
Published: (2009)

Evaluation of modified Congo red agar for detection of biofilm produced by clinical isolates of methicillin–resistance Staphylococcus aureus
by: Shamsudin, Mariana Nor, et al.
Published: (2009)

First community-acquired meticillin-resistant Staphylococcus aureus in Malaysia.
by: Shamsudin, Mariana Nor, et al.
Published: (2008)

Seroprevalence of Leptospira infection in occupational risk groups in North Khorasan province, Iran
by: Hashemi, Seyed Mohammad, et al.
Published: (2021)

Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus
by: Yee, May Ye
Published: (2020)

A Study on the Presence of Pathogenic Leptospira spp. in Environmental Water Samples Obtained from Selected Recreational Areas in Terengganu, Malaysia

Comparison of PCR assay with serum and whole blood samples of experimental trials for detection and differentiation of Brucella melitensis.
by: Takele, Belay Y., et al.
Published: (2009)

Detection and quantification of pathogenic Vibrio parahaemolyticus in shellfish by using multiplex PCR and loop-mediated isothermal amplification assay

Application of polymerase chain reaction assay in detection of Leptospira icterohaemorrhagiae in experimentally infected mice
by: Omar, Sharina, et al.
Published: (2007)

Encapsulated phage cocktail: the destined environmental biocontrol agent for pathogenic leptospira
by: Kokxin, Fang, et al.
Published: (2024)

Detection of pathogenic leptospira in water and soil from selected environment of Sarawak
by: Yong, Ya Fen.
Published: (2015)