Chemical profiles of three varieties of germinated rice based on LC-MS and their antioxidant activity

In this study,chemical profiles in different germinated rice extracts (GREs) using different solvent extraction ratio were investigated.Three varieties of germinated rice (GR), including germinated white rice(GWR), germinated black rice (GBR) and germinated red rice(GRR) were extracted...

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Main Authors: Pramai, Phaiwan, Thongsook, Tipawan, Thanasukarn, Parita, Jannoey, Panatda, Nuengchamnong, Nitra, Feng, Chen, Maulidiani, Maulidiani, Abas, Faridah, Jiamyangyuen, Sudarat
Format: Article
Language:English
Published: Elsevier 2019
Online Access:http://psasir.upm.edu.my/id/eprint/80928/
http://psasir.upm.edu.my/id/eprint/80928/1/RICE.pdf
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author Pramai, Phaiwan
Thongsook, Tipawan
Thanasukarn, Parita
Jannoey, Panatda
Nuengchamnong, Nitra
Feng, Chen
Maulidiani, Maulidiani
Abas, Faridah
Jiamyangyuen, Sudarat
author_facet Pramai, Phaiwan
Thongsook, Tipawan
Thanasukarn, Parita
Jannoey, Panatda
Nuengchamnong, Nitra
Feng, Chen
Maulidiani, Maulidiani
Abas, Faridah
Jiamyangyuen, Sudarat
author_sort Pramai, Phaiwan
building UPM Institutional Repository
collection Online Access
description In this study,chemical profiles in different germinated rice extracts (GREs) using different solvent extraction ratio were investigated.Three varieties of germinated rice (GR), including germinated white rice(GWR), germinated black rice (GBR) and germinated red rice(GRR) were extracted using 70and 100% ethanol(v/v). Both extracts were characterized for their chemical profiles using liquid chromatography-electrospray ionization−quadrupole−time−of−flight mass spectrometry (LC−ESI−Q−TOF−MS). The content of γ−aminobutyric acid (GABA), total phenolic content (TPC), and antioxidant activities were also determined. The chemical profiles of GREs are composed of organic acids, amino acids, vitamins, flavonoids,and phenolic compounds. The GABA content of all rice varieties presented the same pattern in both ethanolic extracts. The TPC of GRE extracted by 70% ethanol (v/v) showed significant higher amount than that in the 100%v/vethanolic extract(p<0.05). The highest TPC was obtained from GBR, followed by GRR and GWR, respectively(p<0.05). The antioxidant activity from three assays, including DPPH, ABTS, and FRAP showed higher activities in the 100% v/vethanolic extracts than their 70% v/v counterparts(p<0.05). The phenolic content showed a low positive Pearson correlation with antioxidant activities, however,the strong positive Pearson’s correlation coefficients were observed among these activities (r= 0.846-0.935). The results suggested that the GR was composed of potential bioactive compounds such as GABA and other phytochemical contents possessing high antioxidant bioactivity which can be used as functional food or as part of nutraceutical products.
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institution Universiti Putra Malaysia
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language English
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publishDate 2019
publisher Elsevier
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spelling upm-809282020-10-14T21:46:39Z http://psasir.upm.edu.my/id/eprint/80928/ Chemical profiles of three varieties of germinated rice based on LC-MS and their antioxidant activity Pramai, Phaiwan Thongsook, Tipawan Thanasukarn, Parita Jannoey, Panatda Nuengchamnong, Nitra Feng, Chen Maulidiani, Maulidiani Abas, Faridah Jiamyangyuen, Sudarat In this study,chemical profiles in different germinated rice extracts (GREs) using different solvent extraction ratio were investigated.Three varieties of germinated rice (GR), including germinated white rice(GWR), germinated black rice (GBR) and germinated red rice(GRR) were extracted using 70and 100% ethanol(v/v). Both extracts were characterized for their chemical profiles using liquid chromatography-electrospray ionization−quadrupole−time−of−flight mass spectrometry (LC−ESI−Q−TOF−MS). The content of γ−aminobutyric acid (GABA), total phenolic content (TPC), and antioxidant activities were also determined. The chemical profiles of GREs are composed of organic acids, amino acids, vitamins, flavonoids,and phenolic compounds. The GABA content of all rice varieties presented the same pattern in both ethanolic extracts. The TPC of GRE extracted by 70% ethanol (v/v) showed significant higher amount than that in the 100%v/vethanolic extract(p<0.05). The highest TPC was obtained from GBR, followed by GRR and GWR, respectively(p<0.05). The antioxidant activity from three assays, including DPPH, ABTS, and FRAP showed higher activities in the 100% v/vethanolic extracts than their 70% v/v counterparts(p<0.05). The phenolic content showed a low positive Pearson correlation with antioxidant activities, however,the strong positive Pearson’s correlation coefficients were observed among these activities (r= 0.846-0.935). The results suggested that the GR was composed of potential bioactive compounds such as GABA and other phytochemical contents possessing high antioxidant bioactivity which can be used as functional food or as part of nutraceutical products. Elsevier 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80928/1/RICE.pdf Pramai, Phaiwan and Thongsook, Tipawan and Thanasukarn, Parita and Jannoey, Panatda and Nuengchamnong, Nitra and Feng, Chen and Maulidiani, Maulidiani and Abas, Faridah and Jiamyangyuen, Sudarat (2019) Chemical profiles of three varieties of germinated rice based on LC-MS and their antioxidant activity. Food and Applied Bioscience Journal, 7 (2). pp. 11-32. ISSN 2212-4292; ESSN: 2212-4306 https://li01.tci-thaijo.org/index.php/fabjournal/article/view/175306/144198
spellingShingle Pramai, Phaiwan
Thongsook, Tipawan
Thanasukarn, Parita
Jannoey, Panatda
Nuengchamnong, Nitra
Feng, Chen
Maulidiani, Maulidiani
Abas, Faridah
Jiamyangyuen, Sudarat
Chemical profiles of three varieties of germinated rice based on LC-MS and their antioxidant activity
title Chemical profiles of three varieties of germinated rice based on LC-MS and their antioxidant activity
title_full Chemical profiles of three varieties of germinated rice based on LC-MS and their antioxidant activity
title_fullStr Chemical profiles of three varieties of germinated rice based on LC-MS and their antioxidant activity
title_full_unstemmed Chemical profiles of three varieties of germinated rice based on LC-MS and their antioxidant activity
title_short Chemical profiles of three varieties of germinated rice based on LC-MS and their antioxidant activity
title_sort chemical profiles of three varieties of germinated rice based on lc-ms and their antioxidant activity
url http://psasir.upm.edu.my/id/eprint/80928/
http://psasir.upm.edu.my/id/eprint/80928/
http://psasir.upm.edu.my/id/eprint/80928/1/RICE.pdf