Biodegradation of hydrocarbon sludge by pseudomonas sp.strain UPM-KV

Ahydrocarbon-utilizing microorganism isolated locallywas characterized and investigated. This study involved standard biochemical tests and investigation of the bacterial growth based on the uptake of carbon and nitrogen source, temperature and optimum pH growth. The bacterium was foun...

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Main Authors: Wasoh @ Mohamad Isa, Helmi, Veeraswamy, Kesavan, Gunasekaran, Baskaran, Abd. Shukor, Mohd. Yunus
Format: Article
Language:English
Published: Hibiscus 2019
Online Access:http://psasir.upm.edu.my/id/eprint/80816/
http://psasir.upm.edu.my/id/eprint/80816/1/DEGRADE.pdf
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author Wasoh @ Mohamad Isa, Helmi
Veeraswamy, Kesavan
Gunasekaran, Baskaran
Abd. Shukor, Mohd. Yunus
author_facet Wasoh @ Mohamad Isa, Helmi
Veeraswamy, Kesavan
Gunasekaran, Baskaran
Abd. Shukor, Mohd. Yunus
author_sort Wasoh @ Mohamad Isa, Helmi
building UPM Institutional Repository
collection Online Access
description Ahydrocarbon-utilizing microorganism isolated locallywas characterized and investigated. This study involved standard biochemical tests and investigation of the bacterial growth based on the uptake of carbon and nitrogen source, temperature and optimum pH growth. The bacterium was found to be a Gram-negative rod, non-motile property with unique property to degrade hydrocarbon sludge. By using diesel as sole carbon source the bacterium was found to be an aerobe as further proven by the oxidase testing. The optimum conditions for the growth was found to be at 30 °C and pH 6.8 with optimum diesel concentration, 1% (v/v). The bacterium ideally used ammonium sulfate as source of nitrogen and was identified as Pseudomonas sp.strain UPM-KV. The ability of this bacterium to efficiently grow on hydrocarbon sludge makes the bacterium an important tool for bioremediation of this toxic sludge that contains high concentration of heavy metals.
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format Article
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institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T12:21:44Z
publishDate 2019
publisher Hibiscus
recordtype eprints
repository_type Digital Repository
spelling upm-808162020-10-15T22:11:11Z http://psasir.upm.edu.my/id/eprint/80816/ Biodegradation of hydrocarbon sludge by pseudomonas sp.strain UPM-KV Wasoh @ Mohamad Isa, Helmi Veeraswamy, Kesavan Gunasekaran, Baskaran Abd. Shukor, Mohd. Yunus Ahydrocarbon-utilizing microorganism isolated locallywas characterized and investigated. This study involved standard biochemical tests and investigation of the bacterial growth based on the uptake of carbon and nitrogen source, temperature and optimum pH growth. The bacterium was found to be a Gram-negative rod, non-motile property with unique property to degrade hydrocarbon sludge. By using diesel as sole carbon source the bacterium was found to be an aerobe as further proven by the oxidase testing. The optimum conditions for the growth was found to be at 30 °C and pH 6.8 with optimum diesel concentration, 1% (v/v). The bacterium ideally used ammonium sulfate as source of nitrogen and was identified as Pseudomonas sp.strain UPM-KV. The ability of this bacterium to efficiently grow on hydrocarbon sludge makes the bacterium an important tool for bioremediation of this toxic sludge that contains high concentration of heavy metals. Hibiscus 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80816/1/DEGRADE.pdf Wasoh @ Mohamad Isa, Helmi and Veeraswamy, Kesavan and Gunasekaran, Baskaran and Abd. Shukor, Mohd. Yunus (2019) Biodegradation of hydrocarbon sludge by pseudomonas sp.strain UPM-KV. Journal of Environmental Microbiology and Toxicology, 7 (1). pp. 10-15. ISSN 2289-5906 https://journal.hibiscuspublisher.com/index.php/JEMAT/article/view/473/471
spellingShingle Wasoh @ Mohamad Isa, Helmi
Veeraswamy, Kesavan
Gunasekaran, Baskaran
Abd. Shukor, Mohd. Yunus
Biodegradation of hydrocarbon sludge by pseudomonas sp.strain UPM-KV
title Biodegradation of hydrocarbon sludge by pseudomonas sp.strain UPM-KV
title_full Biodegradation of hydrocarbon sludge by pseudomonas sp.strain UPM-KV
title_fullStr Biodegradation of hydrocarbon sludge by pseudomonas sp.strain UPM-KV
title_full_unstemmed Biodegradation of hydrocarbon sludge by pseudomonas sp.strain UPM-KV
title_short Biodegradation of hydrocarbon sludge by pseudomonas sp.strain UPM-KV
title_sort biodegradation of hydrocarbon sludge by pseudomonas sp.strain upm-kv
url http://psasir.upm.edu.my/id/eprint/80816/
http://psasir.upm.edu.my/id/eprint/80816/
http://psasir.upm.edu.my/id/eprint/80816/1/DEGRADE.pdf