Authentication approach using enzyme-linked immunosorbent assay for detection of porcine substances

Food manufacturers across the world commonly add animal substances in their food products. Some food products may contain porcine substances including pork, gelatine, blood and pepsin. These substances significantly affect the texture, colour or taste of the end products. Aside from enhancing sensor...

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Main Authors: Raja Nhari, Raja Mohd Hafidz, Warsanah, Irwan Hanish, Khairil Mokhtar, Nur Fadhilah, Hamid, Muhajir, El Sheikha, Aly Farag
Format: Article
Language:English
Published: Wageningen Academic Publishers 2019
Online Access:http://psasir.upm.edu.my/id/eprint/80772/
http://psasir.upm.edu.my/id/eprint/80772/1/FOOD.pdf
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author Raja Nhari, Raja Mohd Hafidz
Warsanah, Irwan Hanish
Khairil Mokhtar, Nur Fadhilah
Hamid, Muhajir
El Sheikha, Aly Farag
author_facet Raja Nhari, Raja Mohd Hafidz
Warsanah, Irwan Hanish
Khairil Mokhtar, Nur Fadhilah
Hamid, Muhajir
El Sheikha, Aly Farag
author_sort Raja Nhari, Raja Mohd Hafidz
building UPM Institutional Repository
collection Online Access
description Food manufacturers across the world commonly add animal substances in their food products. Some food products may contain porcine substances including pork, gelatine, blood and pepsin. These substances significantly affect the texture, colour or taste of the end products. Aside from enhancing sensory qualities, additional ingredients also contribute to preservation, bulk and nutrition. However, the inclusion of porcine substances might not be suitable among certain communities. One primary concern is fraud labelling which includes hiding the addition of porcine substances in food. Therefore, analytical techniques such as enzyme-linked immunosorbent assay (ELISA) have been developed to detect the porcine proteins in food. The ELISA delivers specificity and sensitivity in detecting the targeted animal species in food. This review provides an overview of the ELISA technique which has been developed for potential detection of porcine substances in laboratory-prepared food samples and commercial food products.
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institution Universiti Putra Malaysia
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language English
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publishDate 2019
publisher Wageningen Academic Publishers
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spelling upm-807722020-10-15T22:25:00Z http://psasir.upm.edu.my/id/eprint/80772/ Authentication approach using enzyme-linked immunosorbent assay for detection of porcine substances Raja Nhari, Raja Mohd Hafidz Warsanah, Irwan Hanish Khairil Mokhtar, Nur Fadhilah Hamid, Muhajir El Sheikha, Aly Farag Food manufacturers across the world commonly add animal substances in their food products. Some food products may contain porcine substances including pork, gelatine, blood and pepsin. These substances significantly affect the texture, colour or taste of the end products. Aside from enhancing sensory qualities, additional ingredients also contribute to preservation, bulk and nutrition. However, the inclusion of porcine substances might not be suitable among certain communities. One primary concern is fraud labelling which includes hiding the addition of porcine substances in food. Therefore, analytical techniques such as enzyme-linked immunosorbent assay (ELISA) have been developed to detect the porcine proteins in food. The ELISA delivers specificity and sensitivity in detecting the targeted animal species in food. This review provides an overview of the ELISA technique which has been developed for potential detection of porcine substances in laboratory-prepared food samples and commercial food products. Wageningen Academic Publishers 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80772/1/FOOD.pdf Raja Nhari, Raja Mohd Hafidz and Warsanah, Irwan Hanish and Khairil Mokhtar, Nur Fadhilah and Hamid, Muhajir and El Sheikha, Aly Farag (2019) Authentication approach using enzyme-linked immunosorbent assay for detection of porcine substances. Quality Assurance and Safety of Crops and Foods, 11 (5). pp. 449-457. ISSN 1757-8361; ESSN: 1757-837X https://www.wageningenacademic.com/doi/abs/10.3920/QAS2018.1415 10.3920/QAS2018.1415
spellingShingle Raja Nhari, Raja Mohd Hafidz
Warsanah, Irwan Hanish
Khairil Mokhtar, Nur Fadhilah
Hamid, Muhajir
El Sheikha, Aly Farag
Authentication approach using enzyme-linked immunosorbent assay for detection of porcine substances
title Authentication approach using enzyme-linked immunosorbent assay for detection of porcine substances
title_full Authentication approach using enzyme-linked immunosorbent assay for detection of porcine substances
title_fullStr Authentication approach using enzyme-linked immunosorbent assay for detection of porcine substances
title_full_unstemmed Authentication approach using enzyme-linked immunosorbent assay for detection of porcine substances
title_short Authentication approach using enzyme-linked immunosorbent assay for detection of porcine substances
title_sort authentication approach using enzyme-linked immunosorbent assay for detection of porcine substances
url http://psasir.upm.edu.my/id/eprint/80772/
http://psasir.upm.edu.my/id/eprint/80772/
http://psasir.upm.edu.my/id/eprint/80772/
http://psasir.upm.edu.my/id/eprint/80772/1/FOOD.pdf