Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE
Toxicity evaluation of wastewater, polluted sediment and water streams is a very crucial aspect of environmental pollution monitoring. In this work, a newly developed luminescent bacterial test using a tropical luminescent bacterium, Photobacterium sp. strain MIE was used to assess the toxicity of s...
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| Format: | Article |
| Language: | English |
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Springer
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80742/ http://psasir.upm.edu.my/id/eprint/80742/1/TOXIC.pdf |
| _version_ | 1848858949290295296 |
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| author | Halmi, Mohd Izuan Effendi Kassim, Adibah Abd. Shukor, Mohd. Yunus |
| author_facet | Halmi, Mohd Izuan Effendi Kassim, Adibah Abd. Shukor, Mohd. Yunus |
| author_sort | Halmi, Mohd Izuan Effendi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Toxicity evaluation of wastewater, polluted sediment and water streams is a very crucial aspect of environmental pollution monitoring. In this work, a newly developed luminescent bacterial test using a tropical luminescent bacterium, Photobacterium sp. strain MIE was used to assess the toxicity of several heavy metals using a 15-min assay format. The assessment was carried out by exposing strain MIE to different concentrations of heavy metals ranging from 0.001 to 200 mg/L in a DTX microplate 96 wells. The toxicity result based on the inhibitory concentration (IC50) was Hg (0.053 mg/L) > Ag (0.12 mg/L) > Cu (0.85 mg/L) > Ni (12.32 mg/L) > Zn (18.72 mg/L) > Cr (26.02 mg/L). Principal Component Analysis (PCA) and Agglomerative Hierarchical Clustering (AHC) analyses showed the sensitivity (IC50) of strain MIE to several toxic heavy metals are comparable to the commercial luminescent assay, Microtox™ as both clusters together making it a good choice for an alternative near-real-time monitoring of heavy metals. The sensitivity of strain MIE towards heavy metals was proven through field trial works on several heavy metal-polluted sites in Malaysia. Thus, it is a good candidate as an early detection system for heavy metals in aquatic bodies in tropical countries. |
| first_indexed | 2025-11-15T12:21:34Z |
| format | Article |
| id | upm-80742 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:21:34Z |
| publishDate | 2019 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-807422021-01-23T21:05:30Z http://psasir.upm.edu.my/id/eprint/80742/ Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE Halmi, Mohd Izuan Effendi Kassim, Adibah Abd. Shukor, Mohd. Yunus Toxicity evaluation of wastewater, polluted sediment and water streams is a very crucial aspect of environmental pollution monitoring. In this work, a newly developed luminescent bacterial test using a tropical luminescent bacterium, Photobacterium sp. strain MIE was used to assess the toxicity of several heavy metals using a 15-min assay format. The assessment was carried out by exposing strain MIE to different concentrations of heavy metals ranging from 0.001 to 200 mg/L in a DTX microplate 96 wells. The toxicity result based on the inhibitory concentration (IC50) was Hg (0.053 mg/L) > Ag (0.12 mg/L) > Cu (0.85 mg/L) > Ni (12.32 mg/L) > Zn (18.72 mg/L) > Cr (26.02 mg/L). Principal Component Analysis (PCA) and Agglomerative Hierarchical Clustering (AHC) analyses showed the sensitivity (IC50) of strain MIE to several toxic heavy metals are comparable to the commercial luminescent assay, Microtox™ as both clusters together making it a good choice for an alternative near-real-time monitoring of heavy metals. The sensitivity of strain MIE towards heavy metals was proven through field trial works on several heavy metal-polluted sites in Malaysia. Thus, it is a good candidate as an early detection system for heavy metals in aquatic bodies in tropical countries. Springer 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80742/1/TOXIC.pdf Halmi, Mohd Izuan Effendi and Kassim, Adibah and Abd. Shukor, Mohd. Yunus (2019) Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE. Rendiconti Lincei. Scienze Fisiche e Naturali, 30 (3). pp. 358-601. ISSN 1720-0775 https://link.springer.com/article/10.1007/s12210-019-00809-5 |
| spellingShingle | Halmi, Mohd Izuan Effendi Kassim, Adibah Abd. Shukor, Mohd. Yunus Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE |
| title | Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE |
| title_full | Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE |
| title_fullStr | Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE |
| title_full_unstemmed | Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE |
| title_short | Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE |
| title_sort | assessment of heavy metal toxicity using a luminescent bacterial test based on photobacterium sp. strain mie |
| url | http://psasir.upm.edu.my/id/eprint/80742/ http://psasir.upm.edu.my/id/eprint/80742/ http://psasir.upm.edu.my/id/eprint/80742/1/TOXIC.pdf |