An improved method for the rescue of of recombinant Newcastle disease virus
Reverse genetics has been used to generate recombinant Newcastle disease virus with enhanced immunogenic properties for vaccine development. The system, which involves co-transfecting the viral antigenomic plasmid with three helper plasmids into a T7 RNA polymerase-expressing cell to produce viral p...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Future Science
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80599/ http://psasir.upm.edu.my/id/eprint/80599/1/VIRUS.pdf |
| _version_ | 1848858923062263808 |
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| author | Cheow, Pheik Sheen Tan, Tiong Kit Song, Adelene Ai Lian Yusoff, Khatijah Chia, Suet Lin |
| author_facet | Cheow, Pheik Sheen Tan, Tiong Kit Song, Adelene Ai Lian Yusoff, Khatijah Chia, Suet Lin |
| author_sort | Cheow, Pheik Sheen |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Reverse genetics has been used to generate recombinant Newcastle disease virus with enhanced immunogenic properties for vaccine development. The system, which involves co-transfecting the viral antigenomic plasmid with three helper plasmids into a T7 RNA polymerase-expressing cell to produce viral progenies, poses a great challenge. We have modified the standard transfection method to improve the transfection efficiency of the plasmids, resulting in a higher titer of virus progeny production. Two transfection reagents (i.e., lipofectamine and polyethylenimine) were used to compare the transfection efficiency of the four plasmids. The virus progenies produced were quantitated with flow cytometry analysis of the infectious virus unit. The modified transfection method increased the titer of virus progenies compared with that of the standard transfection method. |
| first_indexed | 2025-11-15T12:21:09Z |
| format | Article |
| id | upm-80599 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:21:09Z |
| publishDate | 2019 |
| publisher | Future Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-805992020-11-05T18:45:27Z http://psasir.upm.edu.my/id/eprint/80599/ An improved method for the rescue of of recombinant Newcastle disease virus Cheow, Pheik Sheen Tan, Tiong Kit Song, Adelene Ai Lian Yusoff, Khatijah Chia, Suet Lin Reverse genetics has been used to generate recombinant Newcastle disease virus with enhanced immunogenic properties for vaccine development. The system, which involves co-transfecting the viral antigenomic plasmid with three helper plasmids into a T7 RNA polymerase-expressing cell to produce viral progenies, poses a great challenge. We have modified the standard transfection method to improve the transfection efficiency of the plasmids, resulting in a higher titer of virus progeny production. Two transfection reagents (i.e., lipofectamine and polyethylenimine) were used to compare the transfection efficiency of the four plasmids. The virus progenies produced were quantitated with flow cytometry analysis of the infectious virus unit. The modified transfection method increased the titer of virus progenies compared with that of the standard transfection method. Future Science 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80599/1/VIRUS.pdf Cheow, Pheik Sheen and Tan, Tiong Kit and Song, Adelene Ai Lian and Yusoff, Khatijah and Chia, Suet Lin (2019) An improved method for the rescue of of recombinant Newcastle disease virus. BioTechniques, 68 (2). pp. 96-100. ISSN 0736-6205; ESSN: 1940-9818 https://pubmed.ncbi.nlm.nih.gov/31937115/ 10.2144/btn-2019-0110 |
| spellingShingle | Cheow, Pheik Sheen Tan, Tiong Kit Song, Adelene Ai Lian Yusoff, Khatijah Chia, Suet Lin An improved method for the rescue of of recombinant Newcastle disease virus |
| title | An improved method for the rescue of of recombinant Newcastle disease virus |
| title_full | An improved method for the rescue of of recombinant Newcastle disease virus |
| title_fullStr | An improved method for the rescue of of recombinant Newcastle disease virus |
| title_full_unstemmed | An improved method for the rescue of of recombinant Newcastle disease virus |
| title_short | An improved method for the rescue of of recombinant Newcastle disease virus |
| title_sort | improved method for the rescue of of recombinant newcastle disease virus |
| url | http://psasir.upm.edu.my/id/eprint/80599/ http://psasir.upm.edu.my/id/eprint/80599/ http://psasir.upm.edu.my/id/eprint/80599/ http://psasir.upm.edu.my/id/eprint/80599/1/VIRUS.pdf |