Acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection
An optical genosensor based on Schiff base complex (Zn2+ salphen) DNA label and acrylic microspheres (AMs) as polymer support of the capturing DNA probe (cpDNA) was developed for dengue virus serotype 2 (DEN-2) detection via reflectance spectrophotometric method. The solid-state optical DNA biosenso...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80534/ http://psasir.upm.edu.my/id/eprint/80534/1/ACRYLIC.pdf |
| _version_ | 1848858914714550272 |
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| author | Khalid, Bahariah Mazlan, Nur-Fadhilah Tan, Ling Ling Heng, Lee Yook Abdul Karim, Nurul Huda Jamaludin, Nur Diyana Mohd Yusof, Nurul Yuziana Huai, Doris Xia Quay |
| author_facet | Khalid, Bahariah Mazlan, Nur-Fadhilah Tan, Ling Ling Heng, Lee Yook Abdul Karim, Nurul Huda Jamaludin, Nur Diyana Mohd Yusof, Nurul Yuziana Huai, Doris Xia Quay |
| author_sort | Khalid, Bahariah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An optical genosensor based on Schiff base complex (Zn2+ salphen) DNA label and acrylic microspheres (AMs) as polymer support of the capturing DNA probe (cpDNA) was developed for dengue virus serotype 2 (DEN-2) detection via reflectance spectrophotometric method. The solid-state optical DNA biosensor showed high selectivity and specificity up to one-base mismatch in the target DNA sequence owing to the salphen chemical structure that is rich in localized electrons, and allowed π-π stacking interaction between stacked base pairs of double-stranded DNA (dsDNA). The reflectometric DNA microsensor demonstrated a broad linear detection range towards DEN-2 DNA from 1 × 10–15 M to 1 × 10−3 M with a low limit of detection (LOD) obtained at 1.21 × 10–16 M. The DNA biosensor gave reproducible optical response with a satisfactory relative standard deviation (RSD) at 3.1%, (n = 3), and the reflectance response was stable even after four regeneration cycles of the DNA biosensor. The optical genosensor was proven comparable with standard reverse transcription polymerase chain reaction (RT-PCR) in detecting DEN-2 genome acquired from clinical samples of serum, urine and saliva of dengue virus infected patients under informed consent. The developed reflectometric DNA biosensor is advantageous in offering an early DEN-2 diagnosis, when fever symptom started to manifest in patient. |
| first_indexed | 2025-11-15T12:21:01Z |
| format | Article |
| id | upm-80534 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:21:01Z |
| publishDate | 2019 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-805342020-11-09T15:30:05Z http://psasir.upm.edu.my/id/eprint/80534/ Acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection Khalid, Bahariah Mazlan, Nur-Fadhilah Tan, Ling Ling Heng, Lee Yook Abdul Karim, Nurul Huda Jamaludin, Nur Diyana Mohd Yusof, Nurul Yuziana Huai, Doris Xia Quay An optical genosensor based on Schiff base complex (Zn2+ salphen) DNA label and acrylic microspheres (AMs) as polymer support of the capturing DNA probe (cpDNA) was developed for dengue virus serotype 2 (DEN-2) detection via reflectance spectrophotometric method. The solid-state optical DNA biosensor showed high selectivity and specificity up to one-base mismatch in the target DNA sequence owing to the salphen chemical structure that is rich in localized electrons, and allowed π-π stacking interaction between stacked base pairs of double-stranded DNA (dsDNA). The reflectometric DNA microsensor demonstrated a broad linear detection range towards DEN-2 DNA from 1 × 10–15 M to 1 × 10−3 M with a low limit of detection (LOD) obtained at 1.21 × 10–16 M. The DNA biosensor gave reproducible optical response with a satisfactory relative standard deviation (RSD) at 3.1%, (n = 3), and the reflectance response was stable even after four regeneration cycles of the DNA biosensor. The optical genosensor was proven comparable with standard reverse transcription polymerase chain reaction (RT-PCR) in detecting DEN-2 genome acquired from clinical samples of serum, urine and saliva of dengue virus infected patients under informed consent. The developed reflectometric DNA biosensor is advantageous in offering an early DEN-2 diagnosis, when fever symptom started to manifest in patient. Elsevier 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80534/1/ACRYLIC.pdf Khalid, Bahariah and Mazlan, Nur-Fadhilah and Tan, Ling Ling and Heng, Lee Yook and Abdul Karim, Nurul Huda and Jamaludin, Nur Diyana and Mohd Yusof, Nurul Yuziana and Huai, Doris Xia Quay (2019) Acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection. Talanta, 198. pp. 358-370. ISSN 0039-9140; ESSN: 1873-3573 https://www.sciencedirect.com/science/article/pii/S0039914019301729 10.1016/j.talanta.2019.02.036 |
| spellingShingle | Khalid, Bahariah Mazlan, Nur-Fadhilah Tan, Ling Ling Heng, Lee Yook Abdul Karim, Nurul Huda Jamaludin, Nur Diyana Mohd Yusof, Nurul Yuziana Huai, Doris Xia Quay Acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection |
| title | Acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection |
| title_full | Acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection |
| title_fullStr | Acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection |
| title_full_unstemmed | Acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection |
| title_short | Acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection |
| title_sort | acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection |
| url | http://psasir.upm.edu.my/id/eprint/80534/ http://psasir.upm.edu.my/id/eprint/80534/ http://psasir.upm.edu.my/id/eprint/80534/ http://psasir.upm.edu.my/id/eprint/80534/1/ACRYLIC.pdf |