Isolation of Janthinobacterium lividum from early onset neonatal sepsis in Malaysia
Background: The term early onset neonatal septicaemia (EONS) refers to invasive bacterial infections that primarily involve the blood stream of neonates during the rst 3 days of life. Although early onset neonatal septicaemia is relatively uncommon, it may be associated with case fatality rates o...
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| Format: | Article |
| Language: | English |
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Makerere University
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80337/ http://psasir.upm.edu.my/id/eprint/80337/1/Isolation%20of%20Janthinobacterium%20lividum%20from%20early%20onset%20neonatal%20sepsis%20in%20Malaysia.pdf |
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| author | Shinkaf, Sa'adatu haruna Umar, Shu'aibu Neela, Vasantha Kumari Md Noor, Sabariah Amin Nordin, Syafinas Hudu, Shuaibu Abdullahi Zainudin, Zurina |
| author_facet | Shinkaf, Sa'adatu haruna Umar, Shu'aibu Neela, Vasantha Kumari Md Noor, Sabariah Amin Nordin, Syafinas Hudu, Shuaibu Abdullahi Zainudin, Zurina |
| author_sort | Shinkaf, Sa'adatu haruna |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: The term early onset neonatal septicaemia (EONS) refers to invasive bacterial infections that primarily involve the blood stream of neonates during the rst 3 days of life. Although early onset neonatal septicaemia is relatively uncommon, it may be associated with case fatality rates of 15-30% and substantial morbidity in surviving infants.
Objectives: This study describes an unusual septicaemia cases with Janthinobacterium lividum in neonatal Intensive Care Units. Methods: Bacterial causes of early onset neonatal sepsis in Kuala Lumpur Hospital Malaysia were investigated using broad range 16S rDNA PCR and sequencing. The bacterial DNA was isolated directly from blood without pre-incubation. All samples collected were equally cultured and incubated in automated BACTEC system. Results: Two hundred and fty two neonates were recruited in this study with mean (SD) gestational age of 35.9. Neonates with J. lividum infection lacked microbiological evidence of septicaemia as their blood culture yielded no bacterial growth. However, the PCR analysis of these samples yielded 1100bp corresponding to bacteria species. Conclusion: This study demonstrates the value of PCR in detecting bacteria where special growth requirement is involved. |
| first_indexed | 2025-11-15T12:20:40Z |
| format | Article |
| id | upm-80337 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:20:40Z |
| publishDate | 2019 |
| publisher | Makerere University |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-803372020-10-21T19:42:57Z http://psasir.upm.edu.my/id/eprint/80337/ Isolation of Janthinobacterium lividum from early onset neonatal sepsis in Malaysia Shinkaf, Sa'adatu haruna Umar, Shu'aibu Neela, Vasantha Kumari Md Noor, Sabariah Amin Nordin, Syafinas Hudu, Shuaibu Abdullahi Zainudin, Zurina Background: The term early onset neonatal septicaemia (EONS) refers to invasive bacterial infections that primarily involve the blood stream of neonates during the rst 3 days of life. Although early onset neonatal septicaemia is relatively uncommon, it may be associated with case fatality rates of 15-30% and substantial morbidity in surviving infants. Objectives: This study describes an unusual septicaemia cases with Janthinobacterium lividum in neonatal Intensive Care Units. Methods: Bacterial causes of early onset neonatal sepsis in Kuala Lumpur Hospital Malaysia were investigated using broad range 16S rDNA PCR and sequencing. The bacterial DNA was isolated directly from blood without pre-incubation. All samples collected were equally cultured and incubated in automated BACTEC system. Results: Two hundred and fty two neonates were recruited in this study with mean (SD) gestational age of 35.9. Neonates with J. lividum infection lacked microbiological evidence of septicaemia as their blood culture yielded no bacterial growth. However, the PCR analysis of these samples yielded 1100bp corresponding to bacteria species. Conclusion: This study demonstrates the value of PCR in detecting bacteria where special growth requirement is involved. Makerere University 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80337/1/Isolation%20of%20Janthinobacterium%20lividum%20from%20early%20onset%20neonatal%20sepsis%20in%20Malaysia.pdf Shinkaf, Sa'adatu haruna and Umar, Shu'aibu and Neela, Vasantha Kumari and Md Noor, Sabariah and Amin Nordin, Syafinas and Hudu, Shuaibu Abdullahi and Zainudin, Zurina (2019) Isolation of Janthinobacterium lividum from early onset neonatal sepsis in Malaysia. African Health Sciences, 19 (3). pp. 2378-2389. ISSN 1680-6905 https://www.researchgate.net/publication/337211865_Isolation_of_Janthinobacterium_lividum_from_early_onset_neonatal_sepsis_patients_in_Malaysia 10.4314/ahs.v19i3.11 |
| spellingShingle | Shinkaf, Sa'adatu haruna Umar, Shu'aibu Neela, Vasantha Kumari Md Noor, Sabariah Amin Nordin, Syafinas Hudu, Shuaibu Abdullahi Zainudin, Zurina Isolation of Janthinobacterium lividum from early onset neonatal sepsis in Malaysia |
| title | Isolation of Janthinobacterium lividum from early onset neonatal sepsis in Malaysia |
| title_full | Isolation of Janthinobacterium lividum from early onset neonatal sepsis in Malaysia |
| title_fullStr | Isolation of Janthinobacterium lividum from early onset neonatal sepsis in Malaysia |
| title_full_unstemmed | Isolation of Janthinobacterium lividum from early onset neonatal sepsis in Malaysia |
| title_short | Isolation of Janthinobacterium lividum from early onset neonatal sepsis in Malaysia |
| title_sort | isolation of janthinobacterium lividum from early onset neonatal sepsis in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/80337/ http://psasir.upm.edu.my/id/eprint/80337/ http://psasir.upm.edu.my/id/eprint/80337/ http://psasir.upm.edu.my/id/eprint/80337/1/Isolation%20of%20Janthinobacterium%20lividum%20from%20early%20onset%20neonatal%20sepsis%20in%20Malaysia.pdf |