Infectious bursal disease virus tissue tropism and pathogenesis of the infection in chickens by application of in situ PCR, immunoperoxase and HE staining
Infectious bursal disease is one of an OIE list of notifiable diseases. Chicken is the only host that manifests clinicalsigns and its pathogenicity is correlated with the distribution of antigens in organs. This study was conducted todetermine disease pathogenesis and virus tissue tropism byin situP...
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Academic Press
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80248/ http://psasir.upm.edu.my/id/eprint/80248/1/Infectious%20bursal%20disease%20virus%20tissue%20tropism%20and%20pathogenesis%20of%20the%20infection%20in%20chickens%20by%20application%20of%20in%20situ%20PCR%2C%20immunoperoxase%20and%20HE%20staining.pdf |
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| author | Elawad, Hussein Abdallah Bejo, Mohd Hair Liew, Pit Sze Adamu, Lawan Omar, Abdul Rahman Arshad, Siti Suri Mohammed, Majed H. Ideris, Aini |
| author_facet | Elawad, Hussein Abdallah Bejo, Mohd Hair Liew, Pit Sze Adamu, Lawan Omar, Abdul Rahman Arshad, Siti Suri Mohammed, Majed H. Ideris, Aini |
| author_sort | Elawad, Hussein Abdallah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Infectious bursal disease is one of an OIE list of notifiable diseases. Chicken is the only host that manifests clinicalsigns and its pathogenicity is correlated with the distribution of antigens in organs. This study was conducted todetermine disease pathogenesis and virus tissue tropism byin situPCR, immunoperoxidase staining (IPS), and HEstaining. Twenty four chickens were infected with very virulent Infectious Bursal Disease Virus (vvIBDV). Fifteenchickens were kept as a control group. Infected chickens were sacrificed at hrs 2, 4, 6, 12, days 1, 2, 4, and 6post-inoculation (pi). While, control chickens were euthanized on days 0, 1, 2, 4, and 6 pi. Different tissues werecollected,fixed in 10% buffered formalin, and processed. At hr 2 pi, virus was detected in intestinal, junction ofthe proventriculus and gizzard, cecal tonsil, liver, kidney, and bursa of Fabricius. At hr 4 pi, virus reached spleen,and at hr 6 pi, it entered thymus. At hr 12 pi, virus concentration increased in positive tissues. The latest invadedtissue was muscle on day 1 pi. Secondary viraemia occurred during 12–24 h pi.In situPCR was the most sensitivetechnique to highlight obscure points of infection in this study. |
| first_indexed | 2025-11-15T12:20:17Z |
| format | Article |
| id | upm-80248 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:20:17Z |
| publishDate | 2019 |
| publisher | Academic Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-802482020-10-19T17:12:48Z http://psasir.upm.edu.my/id/eprint/80248/ Infectious bursal disease virus tissue tropism and pathogenesis of the infection in chickens by application of in situ PCR, immunoperoxase and HE staining Elawad, Hussein Abdallah Bejo, Mohd Hair Liew, Pit Sze Adamu, Lawan Omar, Abdul Rahman Arshad, Siti Suri Mohammed, Majed H. Ideris, Aini Infectious bursal disease is one of an OIE list of notifiable diseases. Chicken is the only host that manifests clinicalsigns and its pathogenicity is correlated with the distribution of antigens in organs. This study was conducted todetermine disease pathogenesis and virus tissue tropism byin situPCR, immunoperoxidase staining (IPS), and HEstaining. Twenty four chickens were infected with very virulent Infectious Bursal Disease Virus (vvIBDV). Fifteenchickens were kept as a control group. Infected chickens were sacrificed at hrs 2, 4, 6, 12, days 1, 2, 4, and 6post-inoculation (pi). While, control chickens were euthanized on days 0, 1, 2, 4, and 6 pi. Different tissues werecollected,fixed in 10% buffered formalin, and processed. At hr 2 pi, virus was detected in intestinal, junction ofthe proventriculus and gizzard, cecal tonsil, liver, kidney, and bursa of Fabricius. At hr 4 pi, virus reached spleen,and at hr 6 pi, it entered thymus. At hr 12 pi, virus concentration increased in positive tissues. The latest invadedtissue was muscle on day 1 pi. Secondary viraemia occurred during 12–24 h pi.In situPCR was the most sensitivetechnique to highlight obscure points of infection in this study. Academic Press 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80248/1/Infectious%20bursal%20disease%20virus%20tissue%20tropism%20and%20pathogenesis%20of%20the%20infection%20in%20chickens%20by%20application%20of%20in%20situ%20PCR%2C%20immunoperoxase%20and%20HE%20staining.pdf Elawad, Hussein Abdallah and Bejo, Mohd Hair and Liew, Pit Sze and Adamu, Lawan and Omar, Abdul Rahman and Arshad, Siti Suri and Mohammed, Majed H. and Ideris, Aini (2019) Infectious bursal disease virus tissue tropism and pathogenesis of the infection in chickens by application of in situ PCR, immunoperoxase and HE staining. Microbial Pathogenesis, 129. pp. 195-205. ISSN 1096-1208; 0882-4010 https://reader.elsevier.com/reader/sd/pii/S0882401018310143?token=FB7CD37CE1850234D963C4FB0E232EC0C65B10CD324F4322B83EEE40A3A19D5B4104BAD8CAF05CB64CE68D7C617D66E9 10.1016/j.micpath.2019.01.049 |
| spellingShingle | Elawad, Hussein Abdallah Bejo, Mohd Hair Liew, Pit Sze Adamu, Lawan Omar, Abdul Rahman Arshad, Siti Suri Mohammed, Majed H. Ideris, Aini Infectious bursal disease virus tissue tropism and pathogenesis of the infection in chickens by application of in situ PCR, immunoperoxase and HE staining |
| title | Infectious bursal disease virus tissue tropism and pathogenesis of the infection in chickens by application of in situ PCR, immunoperoxase and HE staining |
| title_full | Infectious bursal disease virus tissue tropism and pathogenesis of the infection in chickens by application of in situ PCR, immunoperoxase and HE staining |
| title_fullStr | Infectious bursal disease virus tissue tropism and pathogenesis of the infection in chickens by application of in situ PCR, immunoperoxase and HE staining |
| title_full_unstemmed | Infectious bursal disease virus tissue tropism and pathogenesis of the infection in chickens by application of in situ PCR, immunoperoxase and HE staining |
| title_short | Infectious bursal disease virus tissue tropism and pathogenesis of the infection in chickens by application of in situ PCR, immunoperoxase and HE staining |
| title_sort | infectious bursal disease virus tissue tropism and pathogenesis of the infection in chickens by application of in situ pcr, immunoperoxase and he staining |
| url | http://psasir.upm.edu.my/id/eprint/80248/ http://psasir.upm.edu.my/id/eprint/80248/ http://psasir.upm.edu.my/id/eprint/80248/ http://psasir.upm.edu.my/id/eprint/80248/1/Infectious%20bursal%20disease%20virus%20tissue%20tropism%20and%20pathogenesis%20of%20the%20infection%20in%20chickens%20by%20application%20of%20in%20situ%20PCR%2C%20immunoperoxase%20and%20HE%20staining.pdf |