Increased fucoxanthin in Chaetoceros calcitrans extract exacerbates apoptosis in liver cancer cells via multiple targeted cellular pathways,
In this study, anti-proliferative effects of C. calcitrans extract and its fucoxanthin rich fraction (FxRF) were assessed on human liver HepG2 cancer cell line. Efficacy from each extract was determined by cytotoxicity assay, morphological observation, and cell cycle analysis. Mechanisms of action o...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier BV
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80234/ http://psasir.upm.edu.my/id/eprint/80234/1/Increased%20fucoxanthin%20in%20Chaetoceros%20calcitrans%20extract%20exacerbates%20apoptosis%20in%20liver%20cancer%20cells%20via%20multiple%20targeted%20cellular%20pathways%2C.pdf |
| _version_ | 1848858864135438336 |
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| author | Su, Chern Foo Md. Yusoff, Fatimah Umar Imam, Mustapha Jhi, Biau Foo Ismail, Nor Sharina Azmi, Nur Hanisah Yin, Sim Tor Khong, Nicholas Mun Hoe Ismail, Maznah |
| author_facet | Su, Chern Foo Md. Yusoff, Fatimah Umar Imam, Mustapha Jhi, Biau Foo Ismail, Nor Sharina Azmi, Nur Hanisah Yin, Sim Tor Khong, Nicholas Mun Hoe Ismail, Maznah |
| author_sort | Su, Chern Foo |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, anti-proliferative effects of C. calcitrans extract and its fucoxanthin rich fraction (FxRF) were assessed on human liver HepG2 cancer cell line. Efficacy from each extract was determined by cytotoxicity assay, morphological observation, and cell cycle analysis. Mechanisms of action observed were evaluated using multiplex gene expression analysis. Results showed that CME and FxRF induced cytotoxicity to HepG2 cells in a dose and time-dependent manner. FxRF (IC50: 18.89 μg.mL−1) was found to be significantly more potent than CME (IC50: 87.5 μg.mL−1) (p < 0.05). Gene expression studies revealed that anti-proliferative effects in treated cells by C. calcitrans extracts were mediated partly through the modulation of numerous genes involved in cell signaling (AKT1, ERK1/2, JNK), apoptosis (BAX, BID, Bcl-2, APAF, CYCS) and oxidative stress (SOD1, SOD2, CAT). Overall, C. calcitrans extracts demonstrated effective intervention against HepG2 cancer cells where enhanced apoptotic activities were observed with increased fucoxanthin content. |
| first_indexed | 2025-11-15T12:20:13Z |
| format | Article |
| id | upm-80234 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:20:13Z |
| publishDate | 2019 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-802342020-10-02T08:55:13Z http://psasir.upm.edu.my/id/eprint/80234/ Increased fucoxanthin in Chaetoceros calcitrans extract exacerbates apoptosis in liver cancer cells via multiple targeted cellular pathways, Su, Chern Foo Md. Yusoff, Fatimah Umar Imam, Mustapha Jhi, Biau Foo Ismail, Nor Sharina Azmi, Nur Hanisah Yin, Sim Tor Khong, Nicholas Mun Hoe Ismail, Maznah In this study, anti-proliferative effects of C. calcitrans extract and its fucoxanthin rich fraction (FxRF) were assessed on human liver HepG2 cancer cell line. Efficacy from each extract was determined by cytotoxicity assay, morphological observation, and cell cycle analysis. Mechanisms of action observed were evaluated using multiplex gene expression analysis. Results showed that CME and FxRF induced cytotoxicity to HepG2 cells in a dose and time-dependent manner. FxRF (IC50: 18.89 μg.mL−1) was found to be significantly more potent than CME (IC50: 87.5 μg.mL−1) (p < 0.05). Gene expression studies revealed that anti-proliferative effects in treated cells by C. calcitrans extracts were mediated partly through the modulation of numerous genes involved in cell signaling (AKT1, ERK1/2, JNK), apoptosis (BAX, BID, Bcl-2, APAF, CYCS) and oxidative stress (SOD1, SOD2, CAT). Overall, C. calcitrans extracts demonstrated effective intervention against HepG2 cancer cells where enhanced apoptotic activities were observed with increased fucoxanthin content. Elsevier BV 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80234/1/Increased%20fucoxanthin%20in%20Chaetoceros%20calcitrans%20extract%20exacerbates%20apoptosis%20in%20liver%20cancer%20cells%20via%20multiple%20targeted%20cellular%20pathways%2C.pdf Su, Chern Foo and Md. Yusoff, Fatimah and Umar Imam, Mustapha and Jhi, Biau Foo and Ismail, Nor Sharina and Azmi, Nur Hanisah and Yin, Sim Tor and Khong, Nicholas Mun Hoe and Ismail, Maznah (2019) Increased fucoxanthin in Chaetoceros calcitrans extract exacerbates apoptosis in liver cancer cells via multiple targeted cellular pathways,. Biotechnology Reports, 21. art. no. e00296. pp. 1-10. ISSN 2215-017X https://www.sciencedirect.com/science/article/pii/S2215017X18302443 10.1016/j.btre.2018.e00296 |
| spellingShingle | Su, Chern Foo Md. Yusoff, Fatimah Umar Imam, Mustapha Jhi, Biau Foo Ismail, Nor Sharina Azmi, Nur Hanisah Yin, Sim Tor Khong, Nicholas Mun Hoe Ismail, Maznah Increased fucoxanthin in Chaetoceros calcitrans extract exacerbates apoptosis in liver cancer cells via multiple targeted cellular pathways, |
| title | Increased fucoxanthin in Chaetoceros calcitrans extract exacerbates apoptosis in liver cancer cells via multiple targeted cellular pathways, |
| title_full | Increased fucoxanthin in Chaetoceros calcitrans extract exacerbates apoptosis in liver cancer cells via multiple targeted cellular pathways, |
| title_fullStr | Increased fucoxanthin in Chaetoceros calcitrans extract exacerbates apoptosis in liver cancer cells via multiple targeted cellular pathways, |
| title_full_unstemmed | Increased fucoxanthin in Chaetoceros calcitrans extract exacerbates apoptosis in liver cancer cells via multiple targeted cellular pathways, |
| title_short | Increased fucoxanthin in Chaetoceros calcitrans extract exacerbates apoptosis in liver cancer cells via multiple targeted cellular pathways, |
| title_sort | increased fucoxanthin in chaetoceros calcitrans extract exacerbates apoptosis in liver cancer cells via multiple targeted cellular pathways, |
| url | http://psasir.upm.edu.my/id/eprint/80234/ http://psasir.upm.edu.my/id/eprint/80234/ http://psasir.upm.edu.my/id/eprint/80234/ http://psasir.upm.edu.my/id/eprint/80234/1/Increased%20fucoxanthin%20in%20Chaetoceros%20calcitrans%20extract%20exacerbates%20apoptosis%20in%20liver%20cancer%20cells%20via%20multiple%20targeted%20cellular%20pathways%2C.pdf |