In vitro and in vivo anti-tumour effects of coconut water vinegar on 4T1 breast cancer cells
Background: Coconut water and vinegars have been reported to possess potential anti-tumour and immunostimulatory effects. However, the anti-tumour, anti-inflammatory and immunostimulatory effects of coconut water vinegar have yet to be tested. Objective: This study investigated the in vitro and in v...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Swedish Nutrition Foundation
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80214/ http://psasir.upm.edu.my/id/eprint/80214/1/In%20vitro%20and%20in%20vivo%20anti-tumour%20effects%20of%20coconut%20water%20vinegar%20on%204T1%20breast%20cancer%20cells.pdf |
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| author | Mohamad, Nurul Elyani Yeap, Swee Keong Abu, Nadiah Kian, Lam Lim Zamberi, Nur Rizi Nordin, Noraini Sharifuddin, Shaiful Adzni Long, Kamariah Mohammed Alitheen, Norjahan Banu |
| author_facet | Mohamad, Nurul Elyani Yeap, Swee Keong Abu, Nadiah Kian, Lam Lim Zamberi, Nur Rizi Nordin, Noraini Sharifuddin, Shaiful Adzni Long, Kamariah Mohammed Alitheen, Norjahan Banu |
| author_sort | Mohamad, Nurul Elyani |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Coconut water and vinegars have been reported to possess potential anti-tumour and immunostimulatory effects. However, the anti-tumour, anti-inflammatory and immunostimulatory effects of coconut water vinegar have yet to be tested. Objective: This study investigated the in vitro and in vivo anti-tumour effects of coconut water vinegar on 4T1 breast cancer cells. Methods: The 4T1 cells were treated with freeze-dried coconut water vinegar and subjected to MTT cell viability, BrdU, annexin V/PI apoptosis, cell cycle and wound healing assays for the in vitro analysis. For the in vivo chemopreventive evaluation, mice challenged with 4T1 cells were treated with 0.08or 2.00 mL/kg body weight of fresh coconut water vinegar for 28 days. Tumour weight, apoptosis of tumour cells, metastasis and immunity of untreated mice and coconut water vinegar-treated 4T1 challenged mice were compared. Results Freeze-dried coconut water vinegar reduced the cell viability, induced apoptosis and delayed the wound healing effect of 4T1 cells in vitro. In vivo, coconut water vinegar delayed 4T1 breast cancer progression in mice by inducing apoptosis and delaying the metastasis. Furthermore, coconut water vinegar also promoted immune cell cytotoxicity and production of anticancer cytokines. The results indicate that coconut water vinegar delays breast cancer progression by inducing apoptosis in breast cancer cells, suppressing metastasis and activating anti-tumour immunity. Conclusion: Coconut water vinegar is a potential health food ingredient with a chemopreventive effect. |
| first_indexed | 2025-11-15T12:20:08Z |
| format | Article |
| id | upm-80214 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:20:08Z |
| publishDate | 2019 |
| publisher | Swedish Nutrition Foundation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-802142020-10-02T02:18:17Z http://psasir.upm.edu.my/id/eprint/80214/ In vitro and in vivo anti-tumour effects of coconut water vinegar on 4T1 breast cancer cells Mohamad, Nurul Elyani Yeap, Swee Keong Abu, Nadiah Kian, Lam Lim Zamberi, Nur Rizi Nordin, Noraini Sharifuddin, Shaiful Adzni Long, Kamariah Mohammed Alitheen, Norjahan Banu Background: Coconut water and vinegars have been reported to possess potential anti-tumour and immunostimulatory effects. However, the anti-tumour, anti-inflammatory and immunostimulatory effects of coconut water vinegar have yet to be tested. Objective: This study investigated the in vitro and in vivo anti-tumour effects of coconut water vinegar on 4T1 breast cancer cells. Methods: The 4T1 cells were treated with freeze-dried coconut water vinegar and subjected to MTT cell viability, BrdU, annexin V/PI apoptosis, cell cycle and wound healing assays for the in vitro analysis. For the in vivo chemopreventive evaluation, mice challenged with 4T1 cells were treated with 0.08or 2.00 mL/kg body weight of fresh coconut water vinegar for 28 days. Tumour weight, apoptosis of tumour cells, metastasis and immunity of untreated mice and coconut water vinegar-treated 4T1 challenged mice were compared. Results Freeze-dried coconut water vinegar reduced the cell viability, induced apoptosis and delayed the wound healing effect of 4T1 cells in vitro. In vivo, coconut water vinegar delayed 4T1 breast cancer progression in mice by inducing apoptosis and delaying the metastasis. Furthermore, coconut water vinegar also promoted immune cell cytotoxicity and production of anticancer cytokines. The results indicate that coconut water vinegar delays breast cancer progression by inducing apoptosis in breast cancer cells, suppressing metastasis and activating anti-tumour immunity. Conclusion: Coconut water vinegar is a potential health food ingredient with a chemopreventive effect. Swedish Nutrition Foundation 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80214/1/In%20vitro%20and%20in%20vivo%20anti-tumour%20effects%20of%20coconut%20water%20vinegar%20on%204T1%20breast%20cancer%20cells.pdf Mohamad, Nurul Elyani and Yeap, Swee Keong and Abu, Nadiah and Kian, Lam Lim and Zamberi, Nur Rizi and Nordin, Noraini and Sharifuddin, Shaiful Adzni and Long, Kamariah and Mohammed Alitheen, Norjahan Banu (2019) In vitro and in vivo anti-tumour effects of coconut water vinegar on 4T1 breast cancer cells. Food and Nutrition Research, 63. art. no. 1616. pp. 1-11. ISSN 1654-661X; ESSN: 1654-6628 |
| spellingShingle | Mohamad, Nurul Elyani Yeap, Swee Keong Abu, Nadiah Kian, Lam Lim Zamberi, Nur Rizi Nordin, Noraini Sharifuddin, Shaiful Adzni Long, Kamariah Mohammed Alitheen, Norjahan Banu In vitro and in vivo anti-tumour effects of coconut water vinegar on 4T1 breast cancer cells |
| title | In vitro and in vivo anti-tumour effects of coconut water vinegar on 4T1 breast cancer cells |
| title_full | In vitro and in vivo anti-tumour effects of coconut water vinegar on 4T1 breast cancer cells |
| title_fullStr | In vitro and in vivo anti-tumour effects of coconut water vinegar on 4T1 breast cancer cells |
| title_full_unstemmed | In vitro and in vivo anti-tumour effects of coconut water vinegar on 4T1 breast cancer cells |
| title_short | In vitro and in vivo anti-tumour effects of coconut water vinegar on 4T1 breast cancer cells |
| title_sort | in vitro and in vivo anti-tumour effects of coconut water vinegar on 4t1 breast cancer cells |
| url | http://psasir.upm.edu.my/id/eprint/80214/ http://psasir.upm.edu.my/id/eprint/80214/1/In%20vitro%20and%20in%20vivo%20anti-tumour%20effects%20of%20coconut%20water%20vinegar%20on%204T1%20breast%20cancer%20cells.pdf |