Improvement of phytase biosynthesis by new bacterial isolate, pediococcus pentosaceus c4/1a via continuous cultivation
Phytase producer was selected from five different lactic acid bacteria isolates obtained from the animal faeces sources from laboratory culture collection. From the five isolates, C4/1A showed the highest phytase activity (21.25 U/mL) as compared to others and was identified as Pediococcuspentosaceu...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
2019
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/80196/ http://psasir.upm.edu.my/id/eprint/80196/1/Improvement%20of%20phytase%20biosynthesis%20by%20new%20bacterial%20isolate%2C%20pediococcus%20pentosaceus%20c4%201a%20via%20continuous%20cultivation.pdf |
| _version_ | 1848858853709447168 |
|---|---|
| author | Mohamad, Rosfarizan Raman, Sasirega Liang, Juan Boo Azizi, Susan |
| author_facet | Mohamad, Rosfarizan Raman, Sasirega Liang, Juan Boo Azizi, Susan |
| author_sort | Mohamad, Rosfarizan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Phytase producer was selected from five different lactic acid bacteria isolates obtained from the animal faeces sources from laboratory culture collection. From the five isolates, C4/1A showed the highest phytase activity (21.25 U/mL) as compared to others and was identified as PediococcuspentosaceusC4/1A by 16S rRNA gene sequencing. Optimization of medium formulation and culture conditions was conducted by a conventional method (one-factor-at-one-time) using response surface methodology(RSM). Effects of four parameters were studied on the bacterial growth, reducing sugar and phytase productions. The phytase biosynthesis increased from 21.2 U/mL to 42.3 U/mL using the optimized medium formulation and culture conditions. The RSM suggested that yeast extract, glucose, sodium phytate and inoculum size significantly improved by 99.2% production of phytase. Continuous cultivation was carried out at dilution rate ranging from 0.1 h-1 to 0.4 h-1 and the steady-state of P. pentosaceusC4/1A was achieved after five generations and three residence times. The cultivation was carried out for 48 h and the highest productivity of phytase and cell concentration was obtained at dilution rate 0.3 h-1 which resulted in 8.65 U/mL/h and 0.894 g/L/h, respectively. Throughout the cultivation process, production of phytase was improved by 89.3% from shake-flask experiment to 2L bioreactor. |
| first_indexed | 2025-11-15T12:20:03Z |
| format | Article |
| id | upm-80196 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:20:03Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-801962020-10-02T00:10:08Z http://psasir.upm.edu.my/id/eprint/80196/ Improvement of phytase biosynthesis by new bacterial isolate, pediococcus pentosaceus c4/1a via continuous cultivation Mohamad, Rosfarizan Raman, Sasirega Liang, Juan Boo Azizi, Susan Phytase producer was selected from five different lactic acid bacteria isolates obtained from the animal faeces sources from laboratory culture collection. From the five isolates, C4/1A showed the highest phytase activity (21.25 U/mL) as compared to others and was identified as PediococcuspentosaceusC4/1A by 16S rRNA gene sequencing. Optimization of medium formulation and culture conditions was conducted by a conventional method (one-factor-at-one-time) using response surface methodology(RSM). Effects of four parameters were studied on the bacterial growth, reducing sugar and phytase productions. The phytase biosynthesis increased from 21.2 U/mL to 42.3 U/mL using the optimized medium formulation and culture conditions. The RSM suggested that yeast extract, glucose, sodium phytate and inoculum size significantly improved by 99.2% production of phytase. Continuous cultivation was carried out at dilution rate ranging from 0.1 h-1 to 0.4 h-1 and the steady-state of P. pentosaceusC4/1A was achieved after five generations and three residence times. The cultivation was carried out for 48 h and the highest productivity of phytase and cell concentration was obtained at dilution rate 0.3 h-1 which resulted in 8.65 U/mL/h and 0.894 g/L/h, respectively. Throughout the cultivation process, production of phytase was improved by 89.3% from shake-flask experiment to 2L bioreactor. 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80196/1/Improvement%20of%20phytase%20biosynthesis%20by%20new%20bacterial%20isolate%2C%20pediococcus%20pentosaceus%20c4%201a%20via%20continuous%20cultivation.pdf Mohamad, Rosfarizan and Raman, Sasirega and Liang, Juan Boo and Azizi, Susan (2019) Improvement of phytase biosynthesis by new bacterial isolate, pediococcus pentosaceus c4/1a via continuous cultivation. Journal of Microbiology, Biotechnology and Food Sciences, 8 (5). pp. 1118-1124. ISSN 1338-5178 http://web.b.ebscohost.com/ehost/detail/detail?vid=0&sid=7867a440-7c92-46bf-8996-07e338f1b72b%40pdc-v-sessmgr03&bdata=JnNpdGU9ZWhvc3QtbGl2ZQ%3d%3d#AN=136081671&db=a9h 10.15414/jmbfs.2019.8.5.1118-1124 |
| spellingShingle | Mohamad, Rosfarizan Raman, Sasirega Liang, Juan Boo Azizi, Susan Improvement of phytase biosynthesis by new bacterial isolate, pediococcus pentosaceus c4/1a via continuous cultivation |
| title | Improvement of phytase biosynthesis by new bacterial isolate, pediococcus pentosaceus c4/1a via continuous cultivation |
| title_full | Improvement of phytase biosynthesis by new bacterial isolate, pediococcus pentosaceus c4/1a via continuous cultivation |
| title_fullStr | Improvement of phytase biosynthesis by new bacterial isolate, pediococcus pentosaceus c4/1a via continuous cultivation |
| title_full_unstemmed | Improvement of phytase biosynthesis by new bacterial isolate, pediococcus pentosaceus c4/1a via continuous cultivation |
| title_short | Improvement of phytase biosynthesis by new bacterial isolate, pediococcus pentosaceus c4/1a via continuous cultivation |
| title_sort | improvement of phytase biosynthesis by new bacterial isolate, pediococcus pentosaceus c4/1a via continuous cultivation |
| url | http://psasir.upm.edu.my/id/eprint/80196/ http://psasir.upm.edu.my/id/eprint/80196/ http://psasir.upm.edu.my/id/eprint/80196/ http://psasir.upm.edu.my/id/eprint/80196/1/Improvement%20of%20phytase%20biosynthesis%20by%20new%20bacterial%20isolate%2C%20pediococcus%20pentosaceus%20c4%201a%20via%20continuous%20cultivation.pdf |