Improvement of extracellular secretion efficiency of recombinant protein from Escherichia coli: signal peptide fusion, surfactants addition, and phospholipase

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Improvement of extracellular secretion efficiency of recombinant protein from Escherichia coli: signal peptide fusion, surfactants addition, and phospholipase

The secretion of heterologous proteins into Escherichia coli cell culture media offers significant advantages for downstream processing, which can avoid the production of inclusion bodies, cost and time savings, and endotoxin reduction. However, E. coli does not secrete proteins into the extracellul...

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Bibliographic Details
Main Authors:	Kusuma, Sri Agung Fitri, Ida Parwati, Tina Rostinawati, Rukayadi, Yaya, Subroto, Toto
Format:	Article
Language:	English
Published:	Association of Pharmaceutical Innovators 2019
Online Access:	http://psasir.upm.edu.my/id/eprint/80194/ http://psasir.upm.edu.my/id/eprint/80194/1/Improvement%20of%20extracellular%20secretion%20efficiency%20of%20recombinant%20protein%20from%20Escherichia%20coli%20signal%20peptide%20fusion%2C%20surfactants%20addition%2C%20and%20phospholipase.pdf

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