Improved gel-enhanced liquid chromatography-mass spectrometry by chemometrics for halal proteomics
Numerous analytical methods for the authentication of halal meat are now well established, with gel-enhanced liquid chromatography-mass spectrometry (GeLCMS) being a popular approach. However, the selection of potential protein markers on 1-dimensional gel electrophoresis (1DE) prior to LCMS is cons...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2019
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/80184/ http://psasir.upm.edu.my/id/eprint/80184/1/Improved%20gel-enhanced%20liquid%20chromatography-mass%20spectrometry%20bychemometrics%20for%20halal%20proteomics.pdf |
| _version_ | 1848858850177843200 |
|---|---|
| author | Mohd Yusof, Mohd Hafis Yuswan Wan Kamarudin, Wan Mohd Aizat Mohd Desa, Mohd Nasir Mohd Hashim, Amalia Rahim, Nur Ajreena Mustafa, Shuhaimi Mohamed, Rozi Lamasudin, Dhilia Udie |
| author_facet | Mohd Yusof, Mohd Hafis Yuswan Wan Kamarudin, Wan Mohd Aizat Mohd Desa, Mohd Nasir Mohd Hashim, Amalia Rahim, Nur Ajreena Mustafa, Shuhaimi Mohamed, Rozi Lamasudin, Dhilia Udie |
| author_sort | Mohd Yusof, Mohd Hafis Yuswan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Numerous analytical methods for the authentication of halal meat are now well established, with gel-enhanced liquid chromatography-mass spectrometry (GeLCMS) being a popular approach. However, the selection of potential protein markers on 1-dimensional gel electrophoresis (1DE) prior to LCMS is considered problematic, because using the optical density for the selection process could introduce human error. In this study, an improved GeLCMS method assisted by multivariate principal component analysis (PCA) was developed to identify the potential protein markers for non-halal pork among halal beef and chicken. The improved GeLCMS technique allowed for the confident excising of identified protein bands prior to in-gel tryptic digestion. The inferential protein markers (myofibrillar proteins), which might be present in the samples, were determined based on the identified sequence of peptides. This chemometric-assisted GeLCMS could potentially be used as a guideline to assist chemists in analysis of any gel-based separation of biomolecules, regardless of the field of study |
| first_indexed | 2025-11-15T12:20:00Z |
| format | Article |
| id | upm-80184 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:20:00Z |
| publishDate | 2019 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-801842020-10-01T04:03:03Z http://psasir.upm.edu.my/id/eprint/80184/ Improved gel-enhanced liquid chromatography-mass spectrometry by chemometrics for halal proteomics Mohd Yusof, Mohd Hafis Yuswan Wan Kamarudin, Wan Mohd Aizat Mohd Desa, Mohd Nasir Mohd Hashim, Amalia Rahim, Nur Ajreena Mustafa, Shuhaimi Mohamed, Rozi Lamasudin, Dhilia Udie Numerous analytical methods for the authentication of halal meat are now well established, with gel-enhanced liquid chromatography-mass spectrometry (GeLCMS) being a popular approach. However, the selection of potential protein markers on 1-dimensional gel electrophoresis (1DE) prior to LCMS is considered problematic, because using the optical density for the selection process could introduce human error. In this study, an improved GeLCMS method assisted by multivariate principal component analysis (PCA) was developed to identify the potential protein markers for non-halal pork among halal beef and chicken. The improved GeLCMS technique allowed for the confident excising of identified protein bands prior to in-gel tryptic digestion. The inferential protein markers (myofibrillar proteins), which might be present in the samples, were determined based on the identified sequence of peptides. This chemometric-assisted GeLCMS could potentially be used as a guideline to assist chemists in analysis of any gel-based separation of biomolecules, regardless of the field of study Elsevier BV 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80184/1/Improved%20gel-enhanced%20liquid%20chromatography-mass%20spectrometry%20bychemometrics%20for%20halal%20proteomics.pdf Mohd Yusof, Mohd Hafis Yuswan and Wan Kamarudin, Wan Mohd Aizat and Mohd Desa, Mohd Nasir and Mohd Hashim, Amalia and Rahim, Nur Ajreena and Mustafa, Shuhaimi and Mohamed, Rozi and Lamasudin, Dhilia Udie (2019) Improved gel-enhanced liquid chromatography-mass spectrometry by chemometrics for halal proteomics. Chemometrics and Intelligent Laboratory Systems, 192. art. no. 103825. pp. 1-7. ISSN 0169-7439 https://www.sciencedirect.com/science/article/pii/S0169743918305410 10.1016/j.chemolab.2019.103825 |
| spellingShingle | Mohd Yusof, Mohd Hafis Yuswan Wan Kamarudin, Wan Mohd Aizat Mohd Desa, Mohd Nasir Mohd Hashim, Amalia Rahim, Nur Ajreena Mustafa, Shuhaimi Mohamed, Rozi Lamasudin, Dhilia Udie Improved gel-enhanced liquid chromatography-mass spectrometry by chemometrics for halal proteomics |
| title | Improved gel-enhanced liquid chromatography-mass spectrometry by chemometrics for halal proteomics |
| title_full | Improved gel-enhanced liquid chromatography-mass spectrometry by chemometrics for halal proteomics |
| title_fullStr | Improved gel-enhanced liquid chromatography-mass spectrometry by chemometrics for halal proteomics |
| title_full_unstemmed | Improved gel-enhanced liquid chromatography-mass spectrometry by chemometrics for halal proteomics |
| title_short | Improved gel-enhanced liquid chromatography-mass spectrometry by chemometrics for halal proteomics |
| title_sort | improved gel-enhanced liquid chromatography-mass spectrometry by chemometrics for halal proteomics |
| url | http://psasir.upm.edu.my/id/eprint/80184/ http://psasir.upm.edu.my/id/eprint/80184/ http://psasir.upm.edu.my/id/eprint/80184/ http://psasir.upm.edu.my/id/eprint/80184/1/Improved%20gel-enhanced%20liquid%20chromatography-mass%20spectrometry%20bychemometrics%20for%20halal%20proteomics.pdf |