Improved extracellular secretion of β-cyclodextrin glycosyltransferase from Escherichia coli by glycine supplementation without apparent cell lysis

Improved extracellular secretion of β-cyclodextrin glycosyltransferase from Escherichia coli by glycine supplementation without apparent cell lysis

The use of an effective inducer feeding strategy without causing cell lysis presents significant advantage to enhance the secretion of an enzyme to the culture medium of Escherichia coli. The cgt gene encoding β-cyclodextrin glycosyltransferase (β-CGTase) was cloned into pQE30xa as an N-terminal His...

Full description

Bibliographic Details
Main Authors: Nik Pa, Nik Ida Mardiana, Abd. Aziz, Suraini, Ibrahim, Mohamad Faizal, Mohammed Alitheen, Noorjahan Banu, Ramli, Norhayati
Format: Article
Language:English
Published: Universiti Putra Malaysia 2019
Online Access:http://psasir.upm.edu.my/id/eprint/80183/
http://psasir.upm.edu.my/id/eprint/80183/1/Improved%20extracellular%20secretion%20of%20%CE%B2-cyclodextrin%20glycosyltransferase%20from%20Escherichia%20coli%20by%20glycine%20supplementation%20without%20apparent%20cell%20lysis.pdf

Similar Items

Similar Items