Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms
Gene expression profiling analysis using real-time quantitative polymerase chain reaction (qRT-PCR) is commonly used due to its reliability and accuracy. Gene expression profiles of particular genes in different tissues and developmental stages aid in the understanding of their biological functions....
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| Format: | Article |
| Language: | English |
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Malaysian Palm Oil Board
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80136/ http://psasir.upm.edu.my/id/eprint/80136/1/Identification%20of%20Reliable%20Reference%20Genes%20for%20Gene%20Expression%20Studies%20of%20Oil%20Palm%20Plantlets%20using%20NormFinder%20and%20BestKeeper%20Algorithms.pdf |
| _version_ | 1848858838636167168 |
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| author | A. H., Muhamad Afiq Shaharuddin, Noor Azmi R., Zubaidah |
| author_facet | A. H., Muhamad Afiq Shaharuddin, Noor Azmi R., Zubaidah |
| author_sort | A. H., Muhamad Afiq |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Gene expression profiling analysis using real-time quantitative polymerase chain reaction (qRT-PCR) is commonly used due to its reliability and accuracy. Gene expression profiles of particular genes in different tissues and developmental stages aid in the understanding of their biological functions. However, the accuracy and reliability of qRT-PCR to study gene expression demand the use of species-specific reference genes for normalisation. Therefore, appropriate reference genes for qRT-PCR need to be determined for oil palm ( Elaeis guineensis) tissues. In this study, six candidate reference genes namely actin, Cyp2, GRAS, EgEfa1, F-box, and GLU were examined in leaf, node, internode, root, cabbage, shoot primordial and basal stem tissues of two-year old clonal oil palms. Two statistical algorithms, NormFinder and BestKeeper verified the stability of GRAS and Cyp2 reference genes in the oil palm tissues tested. The combination of Cyp2 and GRAS reference genes are recommended for gene expression normalisation profiles in nurserystage oil palm plantlets. |
| first_indexed | 2025-11-15T12:19:49Z |
| format | Article |
| id | upm-80136 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:19:49Z |
| publishDate | 2019 |
| publisher | Malaysian Palm Oil Board |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-801362020-09-23T08:04:59Z http://psasir.upm.edu.my/id/eprint/80136/ Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms A. H., Muhamad Afiq Shaharuddin, Noor Azmi R., Zubaidah Gene expression profiling analysis using real-time quantitative polymerase chain reaction (qRT-PCR) is commonly used due to its reliability and accuracy. Gene expression profiles of particular genes in different tissues and developmental stages aid in the understanding of their biological functions. However, the accuracy and reliability of qRT-PCR to study gene expression demand the use of species-specific reference genes for normalisation. Therefore, appropriate reference genes for qRT-PCR need to be determined for oil palm ( Elaeis guineensis) tissues. In this study, six candidate reference genes namely actin, Cyp2, GRAS, EgEfa1, F-box, and GLU were examined in leaf, node, internode, root, cabbage, shoot primordial and basal stem tissues of two-year old clonal oil palms. Two statistical algorithms, NormFinder and BestKeeper verified the stability of GRAS and Cyp2 reference genes in the oil palm tissues tested. The combination of Cyp2 and GRAS reference genes are recommended for gene expression normalisation profiles in nurserystage oil palm plantlets. Malaysian Palm Oil Board 2019 Article NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/80136/1/Identification%20of%20Reliable%20Reference%20Genes%20for%20Gene%20Expression%20Studies%20of%20Oil%20Palm%20Plantlets%20using%20NormFinder%20and%20BestKeeper%20Algorithms.pdf A. H., Muhamad Afiq and Shaharuddin, Noor Azmi and R., Zubaidah (2019) Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms. Journal of Oil Palm Research, 31 (2). pp. 1-8. ISSN 1511-2780 10.21894/jopr.2019.0010 |
| spellingShingle | A. H., Muhamad Afiq Shaharuddin, Noor Azmi R., Zubaidah Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms |
| title | Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms |
| title_full | Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms |
| title_fullStr | Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms |
| title_full_unstemmed | Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms |
| title_short | Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms |
| title_sort | identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms |
| url | http://psasir.upm.edu.my/id/eprint/80136/ http://psasir.upm.edu.my/id/eprint/80136/ http://psasir.upm.edu.my/id/eprint/80136/1/Identification%20of%20Reliable%20Reference%20Genes%20for%20Gene%20Expression%20Studies%20of%20Oil%20Palm%20Plantlets%20using%20NormFinder%20and%20BestKeeper%20Algorithms.pdf |