Identification of anti-inflammatory compound/compounds in hexane fraction of Jatropha curcas root extract
Jatropha curcas is a medicinal plant with many therapeutic properties such as anti-inflammatory, anti-malaria, anti-cancer and antioxidant. The root extract has been shown to possess high anti-inflammatory activity. Previously, the compounds responsible for this activity have not been fully elucidat...
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Universiti Putra Malaysia
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80130/ http://psasir.upm.edu.my/id/eprint/80130/1/Identification%20of%20anti-inflammatory%20compoundcompounds%20in%20hexane%20fraction%20of%20Jatropha%20curcas%20root%20extract.pdf |
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| author | Othman, Ahmad Razi Ismail, Intan Safinar Abdullah, Norhani Ahmad, Syahida |
| author_facet | Othman, Ahmad Razi Ismail, Intan Safinar Abdullah, Norhani Ahmad, Syahida |
| author_sort | Othman, Ahmad Razi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Jatropha curcas is a medicinal plant with many therapeutic properties such as anti-inflammatory, anti-malaria, anti-cancer and antioxidant. The root extract has been shown to possess high anti-inflammatory activity. Previously, the compounds responsible for this activity have not been fully elucidated. Two fractions (Fraction 1 and Fraction 2) obtained from a preparative HPLC of the root extract showed significant anti-inflammatory and cytotoxic activities in RAW 264.7 murine macrophage cells with Fraction 1 giving higher nitric oxide (NO) inhibition compared to Fraction 2 and L-NAME. Further purification steps involving column chromatography, thin layer chromatography and analytical HPLC of Fraction 1 produced two fractions labeled as Fraction A and Fraction B. Both fractions showed anti-inflammatory activity without cytotoxic activity in RAW 264.7 cells. Liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis showed that Fraction A contained a group of 18 carbon fatty acid compounds consisting of 2 oxooctadecanoic acids; 15, 16 dihydroxy 9Z, 12Z octadecadienoic acid; octadecadienoic isomer and 15,16 dihydroxy 9Z, 12Z octadecadienoic acid, 15S, 16S. The 18-carbon fatty acid structure was confirmed by nuclear magnetic resonance (NMR) spectral data. The IC₅₀ value of compounds in Fraction A for anti-inflammatory activity in RAW 264.7 cell line was 434.8±0.75 µg/mL. From the analysis, it can be concluded that Fraction A can be classified under 18 carbon long chain fatty acid group based on LC MS/MS and NMR analysis. This active compound shows an inhibition towards NO activity. |
| first_indexed | 2025-11-15T12:19:47Z |
| format | Article |
| id | upm-80130 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:19:47Z |
| publishDate | 2019 |
| publisher | Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-801302020-09-23T02:45:36Z http://psasir.upm.edu.my/id/eprint/80130/ Identification of anti-inflammatory compound/compounds in hexane fraction of Jatropha curcas root extract Othman, Ahmad Razi Ismail, Intan Safinar Abdullah, Norhani Ahmad, Syahida Jatropha curcas is a medicinal plant with many therapeutic properties such as anti-inflammatory, anti-malaria, anti-cancer and antioxidant. The root extract has been shown to possess high anti-inflammatory activity. Previously, the compounds responsible for this activity have not been fully elucidated. Two fractions (Fraction 1 and Fraction 2) obtained from a preparative HPLC of the root extract showed significant anti-inflammatory and cytotoxic activities in RAW 264.7 murine macrophage cells with Fraction 1 giving higher nitric oxide (NO) inhibition compared to Fraction 2 and L-NAME. Further purification steps involving column chromatography, thin layer chromatography and analytical HPLC of Fraction 1 produced two fractions labeled as Fraction A and Fraction B. Both fractions showed anti-inflammatory activity without cytotoxic activity in RAW 264.7 cells. Liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis showed that Fraction A contained a group of 18 carbon fatty acid compounds consisting of 2 oxooctadecanoic acids; 15, 16 dihydroxy 9Z, 12Z octadecadienoic acid; octadecadienoic isomer and 15,16 dihydroxy 9Z, 12Z octadecadienoic acid, 15S, 16S. The 18-carbon fatty acid structure was confirmed by nuclear magnetic resonance (NMR) spectral data. The IC₅₀ value of compounds in Fraction A for anti-inflammatory activity in RAW 264.7 cell line was 434.8±0.75 µg/mL. From the analysis, it can be concluded that Fraction A can be classified under 18 carbon long chain fatty acid group based on LC MS/MS and NMR analysis. This active compound shows an inhibition towards NO activity. Universiti Putra Malaysia 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80130/1/Identification%20of%20anti-inflammatory%20compoundcompounds%20in%20hexane%20fraction%20of%20Jatropha%20curcas%20root%20extract.pdf Othman, Ahmad Razi and Ismail, Intan Safinar and Abdullah, Norhani and Ahmad, Syahida (2019) Identification of anti-inflammatory compound/compounds in hexane fraction of Jatropha curcas root extract. Asia-Pacific Journal of Molecular Biology and Biotechnology, 27 (4). pp. 62-68. ISSN 0128-7451 10.35118/apjmbb.2019.027.4.07 |
| spellingShingle | Othman, Ahmad Razi Ismail, Intan Safinar Abdullah, Norhani Ahmad, Syahida Identification of anti-inflammatory compound/compounds in hexane fraction of Jatropha curcas root extract |
| title | Identification of anti-inflammatory compound/compounds in hexane fraction of Jatropha curcas root extract |
| title_full | Identification of anti-inflammatory compound/compounds in hexane fraction of Jatropha curcas root extract |
| title_fullStr | Identification of anti-inflammatory compound/compounds in hexane fraction of Jatropha curcas root extract |
| title_full_unstemmed | Identification of anti-inflammatory compound/compounds in hexane fraction of Jatropha curcas root extract |
| title_short | Identification of anti-inflammatory compound/compounds in hexane fraction of Jatropha curcas root extract |
| title_sort | identification of anti-inflammatory compound/compounds in hexane fraction of jatropha curcas root extract |
| url | http://psasir.upm.edu.my/id/eprint/80130/ http://psasir.upm.edu.my/id/eprint/80130/ http://psasir.upm.edu.my/id/eprint/80130/1/Identification%20of%20anti-inflammatory%20compoundcompounds%20in%20hexane%20fraction%20of%20Jatropha%20curcas%20root%20extract.pdf |