Identification and application of fungal biocontrol agent cladosporium cladosporioides against bemisia tabaci
The entomopathogenic fungus Cladosporium cladosporioides is a potential candidate for biocontrol of insect pests. We isolated a strain of C. cladosporioides BOU1 from an infected brown plant hoper (BPH) of rice and characterized it using morpho-physiological and molecular analyses. Internal transcri...
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80128/ http://psasir.upm.edu.my/id/eprint/80128/1/Identification%20and%20application%20of%20fungal%20biocontrol%20agent%20cladosporium%20cladosporioides%20against%20bemisia%20tabaci.pdf |
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| author | Islam, Touhidul Gupta, Dipali Rani Surovy, Musrat Zahan Mahmud, Nur Uddin Mazlan, Norida Islam, Tofazzal |
| author_facet | Islam, Touhidul Gupta, Dipali Rani Surovy, Musrat Zahan Mahmud, Nur Uddin Mazlan, Norida Islam, Tofazzal |
| author_sort | Islam, Touhidul |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The entomopathogenic fungus Cladosporium cladosporioides is a potential candidate for biocontrol of insect pests. We isolated a strain of C. cladosporioides BOU1 from an infected brown plant hoper (BPH) of rice and characterized it using morpho-physiological and molecular analyses. Internal transcribed spacer regions and intervening 5.8S rRNA gene (ITS) sequencing and morphopathogenic analyses confirmed that BOU1 is a strain of C. cladosporioides. To select the suitable medium for this fungus, a single condium of BOU1 was grown in potato dextrose agar (PDA), potato dextrose agar with yeast (PDAY), Sabouraud dextrose agar (SDA) and synthetic nutrient-poor agar (SNA) media. The suitable medium for this fungal isolate was determined by fungal growth (colony area and conidiogenesis), and enzymatic activities (protease and lipase). The fungal growth parameters including enzymatic activities showed that the PDA medium is most suitable culture medium for C. cladosporioides. Finally, the pathogenicity of this fungal isolate was evaluated against whitefly, Bemisia tabaci through direct contact toxicity assay on eggplant leaves by dipping under laboratory conditions. The BOU1 strain caused mortality in B. tabaci in a dose-dependent manner, the highest mortality being 71% at 1 × 108 conidia/mL. To the best of our knowledge, this is the first report of isolation and molecular characterization of an entomopathogenic fungus C. cladosporioides from a BPH of rice. This study suggests that BOU1 is a potential candidate for biological control of whitefly for the promotion of sustainable agriculture. |
| first_indexed | 2025-11-15T12:19:47Z |
| format | Article |
| id | upm-80128 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:19:47Z |
| publishDate | 2019 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-801282020-09-22T08:57:16Z http://psasir.upm.edu.my/id/eprint/80128/ Identification and application of fungal biocontrol agent cladosporium cladosporioides against bemisia tabaci Islam, Touhidul Gupta, Dipali Rani Surovy, Musrat Zahan Mahmud, Nur Uddin Mazlan, Norida Islam, Tofazzal The entomopathogenic fungus Cladosporium cladosporioides is a potential candidate for biocontrol of insect pests. We isolated a strain of C. cladosporioides BOU1 from an infected brown plant hoper (BPH) of rice and characterized it using morpho-physiological and molecular analyses. Internal transcribed spacer regions and intervening 5.8S rRNA gene (ITS) sequencing and morphopathogenic analyses confirmed that BOU1 is a strain of C. cladosporioides. To select the suitable medium for this fungus, a single condium of BOU1 was grown in potato dextrose agar (PDA), potato dextrose agar with yeast (PDAY), Sabouraud dextrose agar (SDA) and synthetic nutrient-poor agar (SNA) media. The suitable medium for this fungal isolate was determined by fungal growth (colony area and conidiogenesis), and enzymatic activities (protease and lipase). The fungal growth parameters including enzymatic activities showed that the PDA medium is most suitable culture medium for C. cladosporioides. Finally, the pathogenicity of this fungal isolate was evaluated against whitefly, Bemisia tabaci through direct contact toxicity assay on eggplant leaves by dipping under laboratory conditions. The BOU1 strain caused mortality in B. tabaci in a dose-dependent manner, the highest mortality being 71% at 1 × 108 conidia/mL. To the best of our knowledge, this is the first report of isolation and molecular characterization of an entomopathogenic fungus C. cladosporioides from a BPH of rice. This study suggests that BOU1 is a potential candidate for biological control of whitefly for the promotion of sustainable agriculture. Taylor & Francis 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80128/1/Identification%20and%20application%20of%20fungal%20biocontrol%20agent%20cladosporium%20cladosporioides%20against%20bemisia%20tabaci.pdf Islam, Touhidul and Gupta, Dipali Rani and Surovy, Musrat Zahan and Mahmud, Nur Uddin and Mazlan, Norida and Islam, Tofazzal (2019) Identification and application of fungal biocontrol agent cladosporium cladosporioides against bemisia tabaci. Biotechnology and Biotechnological Equipment, 33 (1). pp. 1698-1705. ISSN 1310-2818 https://www.tandfonline.com/doi/full/10.1080/13102818.2019.1695541 10.1080/13102818.2019.1695541 |
| spellingShingle | Islam, Touhidul Gupta, Dipali Rani Surovy, Musrat Zahan Mahmud, Nur Uddin Mazlan, Norida Islam, Tofazzal Identification and application of fungal biocontrol agent cladosporium cladosporioides against bemisia tabaci |
| title | Identification and application of fungal biocontrol agent cladosporium cladosporioides against bemisia tabaci |
| title_full | Identification and application of fungal biocontrol agent cladosporium cladosporioides against bemisia tabaci |
| title_fullStr | Identification and application of fungal biocontrol agent cladosporium cladosporioides against bemisia tabaci |
| title_full_unstemmed | Identification and application of fungal biocontrol agent cladosporium cladosporioides against bemisia tabaci |
| title_short | Identification and application of fungal biocontrol agent cladosporium cladosporioides against bemisia tabaci |
| title_sort | identification and application of fungal biocontrol agent cladosporium cladosporioides against bemisia tabaci |
| url | http://psasir.upm.edu.my/id/eprint/80128/ http://psasir.upm.edu.my/id/eprint/80128/ http://psasir.upm.edu.my/id/eprint/80128/ http://psasir.upm.edu.my/id/eprint/80128/1/Identification%20and%20application%20of%20fungal%20biocontrol%20agent%20cladosporium%20cladosporioides%20against%20bemisia%20tabaci.pdf |