Human wharton’s jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect
Cardiac c-kit cells show promise in regenerating an injured heart. While heart disease commonly affects elderly patients, it is unclear if autologous cardiac c-kit cells are functionally competent and applicable to these patients. This study characterised cardiac c-kit cells (CCs) from aged mice and...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80102/ http://psasir.upm.edu.my/id/eprint/80102/1/Human%20wharton%E2%80%99s%20jelly-derived%20mesenchymal%20stem%20cells%20minimally%20improve%20the%20growth%20kinetics%20and%20cardiomyocyte%20differentiation%20of%20aged%20murine%20cardiac%20c-kit%20cells%20in%20in%20vitro%20without%20rejuvenating%20effect.pdf |
| _version_ | 1848858829557596160 |
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| author | Ng, Wai Hoe Yong, Yoke Keong Ramasamy, Rajesh Ngalim, Siti Hawa Lim, Vuanghao Shaharuddin, Bakiah Tan, Jun Jie |
| author_facet | Ng, Wai Hoe Yong, Yoke Keong Ramasamy, Rajesh Ngalim, Siti Hawa Lim, Vuanghao Shaharuddin, Bakiah Tan, Jun Jie |
| author_sort | Ng, Wai Hoe |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Cardiac c-kit cells show promise in regenerating an injured heart. While heart disease commonly affects elderly patients, it is unclear if autologous cardiac c-kit cells are functionally competent and applicable to these patients. This study characterised cardiac c-kit cells (CCs) from aged mice and studied the effects of human Wharton’s Jelly-derived mesenchymal stem cells (MSCs) on the growth kinetics and cardiac differentiation of aged CCs in vitro. CCs were isolated from 4-week- and 18-month-old C57/BL6N mice and were directly co-cultured with MSCs or separated by transwell insert. Clonogenically expanded aged CCs showed comparable telomere length to young CCs. However, these cells showed lower Gata4, Nkx2.5, and Sox2 gene expressions, with changes of 2.4, 3767.0, and 4.9 folds, respectively. Direct co-culture of both cells increased aged CC migration, which repopulated 54.6 ± 4.4% of the gap area as compared to aged CCs with MSCs in transwell (42.9 ± 2.6%) and CCs without MSCs (44.7 ± 2.5%). Both direct and transwell co-culture improved proliferation in aged CCs by 15.0% and 16.4%, respectively, as traced using carboxyfluorescein succinimidyl ester (CFSE) for three days. These data suggest that MSCs can improve the growth kinetics of aged CCs. CCs retaining intact telomere are present in old hearts and could be obtained based on their self-renewing capability. Although these aged CCs with reduced growth kinetics are improved by MSCs via cell–cell contact, the effect is minimal. |
| first_indexed | 2025-11-15T12:19:40Z |
| format | Article |
| id | upm-80102 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:19:40Z |
| publishDate | 2019 |
| publisher | MDPI AG |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-801022020-09-22T01:36:52Z http://psasir.upm.edu.my/id/eprint/80102/ Human wharton’s jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect Ng, Wai Hoe Yong, Yoke Keong Ramasamy, Rajesh Ngalim, Siti Hawa Lim, Vuanghao Shaharuddin, Bakiah Tan, Jun Jie Cardiac c-kit cells show promise in regenerating an injured heart. While heart disease commonly affects elderly patients, it is unclear if autologous cardiac c-kit cells are functionally competent and applicable to these patients. This study characterised cardiac c-kit cells (CCs) from aged mice and studied the effects of human Wharton’s Jelly-derived mesenchymal stem cells (MSCs) on the growth kinetics and cardiac differentiation of aged CCs in vitro. CCs were isolated from 4-week- and 18-month-old C57/BL6N mice and were directly co-cultured with MSCs or separated by transwell insert. Clonogenically expanded aged CCs showed comparable telomere length to young CCs. However, these cells showed lower Gata4, Nkx2.5, and Sox2 gene expressions, with changes of 2.4, 3767.0, and 4.9 folds, respectively. Direct co-culture of both cells increased aged CC migration, which repopulated 54.6 ± 4.4% of the gap area as compared to aged CCs with MSCs in transwell (42.9 ± 2.6%) and CCs without MSCs (44.7 ± 2.5%). Both direct and transwell co-culture improved proliferation in aged CCs by 15.0% and 16.4%, respectively, as traced using carboxyfluorescein succinimidyl ester (CFSE) for three days. These data suggest that MSCs can improve the growth kinetics of aged CCs. CCs retaining intact telomere are present in old hearts and could be obtained based on their self-renewing capability. Although these aged CCs with reduced growth kinetics are improved by MSCs via cell–cell contact, the effect is minimal. MDPI AG 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80102/1/Human%20wharton%E2%80%99s%20jelly-derived%20mesenchymal%20stem%20cells%20minimally%20improve%20the%20growth%20kinetics%20and%20cardiomyocyte%20differentiation%20of%20aged%20murine%20cardiac%20c-kit%20cells%20in%20in%20vitro%20without%20rejuvenating%20effect.pdf Ng, Wai Hoe and Yong, Yoke Keong and Ramasamy, Rajesh and Ngalim, Siti Hawa and Lim, Vuanghao and Shaharuddin, Bakiah and Tan, Jun Jie (2019) Human wharton’s jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect. International Journal of Molecular Sciences, 20 (22). art. no. 5519. pp. 1-20. ISSN 1661-6596; ESSN: 1422-0067 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6887783/pdf/ijms-20-05519.pdf 10.3390/ijms20225519 |
| spellingShingle | Ng, Wai Hoe Yong, Yoke Keong Ramasamy, Rajesh Ngalim, Siti Hawa Lim, Vuanghao Shaharuddin, Bakiah Tan, Jun Jie Human wharton’s jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect |
| title | Human wharton’s jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect |
| title_full | Human wharton’s jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect |
| title_fullStr | Human wharton’s jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect |
| title_full_unstemmed | Human wharton’s jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect |
| title_short | Human wharton’s jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect |
| title_sort | human wharton’s jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect |
| url | http://psasir.upm.edu.my/id/eprint/80102/ http://psasir.upm.edu.my/id/eprint/80102/ http://psasir.upm.edu.my/id/eprint/80102/ http://psasir.upm.edu.my/id/eprint/80102/1/Human%20wharton%E2%80%99s%20jelly-derived%20mesenchymal%20stem%20cells%20minimally%20improve%20the%20growth%20kinetics%20and%20cardiomyocyte%20differentiation%20of%20aged%20murine%20cardiac%20c-kit%20cells%20in%20in%20vitro%20without%20rejuvenating%20effect.pdf |