High-efficiency DNA extraction using poly(4,4′-cyclohexylidene bisphenol oxalate)-modified microcrystalline cellulose magnetite composite
In this study, we studied the DNA extraction capability of poly(4,4-cyclohexylidene bisphenol oxalate) following the surface modification and composite formation with that of microcrystalline cellulose (MCC) and magnetic iron oxide nanoparticles (NPs). The physical characterization techniques like s...
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| Format: | Article |
| Language: | English |
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Hindawi Limited
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80064/ http://psasir.upm.edu.my/id/eprint/80064/1/High-efficiency%20DNA%20extraction%20using%20poly%284%2C4%E2%80%B2-cyclohexylidene%20bisphenol%20oxalate%29-modified%20microcrystalline%20cellulose%20magnetite%20composite.pdf |
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| author | Al Balawi, Aisha Nawaf Yusof, Nor Azah Kamaruzaman, Sazlinda Mohammad, Faruq Wasoh @ Mohamad Isa, Helmi Al Lohedan, Hamad A. |
| author_facet | Al Balawi, Aisha Nawaf Yusof, Nor Azah Kamaruzaman, Sazlinda Mohammad, Faruq Wasoh @ Mohamad Isa, Helmi Al Lohedan, Hamad A. |
| author_sort | Al Balawi, Aisha Nawaf |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, we studied the DNA extraction capability of poly(4,4-cyclohexylidene bisphenol oxalate) following the surface modification and composite formation with that of microcrystalline cellulose (MCC) and magnetic iron oxide nanoparticles (NPs). The physical characterization techniques like scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, energy-dispersive X-ray analysis (EDX), and thermogravimetric analysis (TGA) were employed for the poly(bisphenol Z oxalate)-MCC-magnetite composite during different stages of its formation. The results confirmed the successful modification of the polymer surface. On testing in the presence of three types of binding buffers, a high value of 72.4% (out of 10,000 ng/μL) efficiency with a total yield of DNA at ng and absorbance ratio of A260/A280 (1.980) was observed for the 2 M GuHCl/EtOH binding buffer. These results were compared against the other two buffers of phosphate-buffered saline (PBS) and NaCl. The lowest value of DNA extraction efficiency at 8125 ng/μL of 58.845% with absorbance ratios of A260/A280 (1.818) for PBS was also observed. The study has concluded an enhancement in the DNA extraction efficiency when the polymer is in the composite stage along with cellulose and magnetite particles as compared against the bare polymer. |
| first_indexed | 2025-11-15T12:19:31Z |
| format | Article |
| id | upm-80064 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:19:31Z |
| publishDate | 2019 |
| publisher | Hindawi Limited |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-800642020-09-17T03:50:45Z http://psasir.upm.edu.my/id/eprint/80064/ High-efficiency DNA extraction using poly(4,4′-cyclohexylidene bisphenol oxalate)-modified microcrystalline cellulose magnetite composite Al Balawi, Aisha Nawaf Yusof, Nor Azah Kamaruzaman, Sazlinda Mohammad, Faruq Wasoh @ Mohamad Isa, Helmi Al Lohedan, Hamad A. In this study, we studied the DNA extraction capability of poly(4,4-cyclohexylidene bisphenol oxalate) following the surface modification and composite formation with that of microcrystalline cellulose (MCC) and magnetic iron oxide nanoparticles (NPs). The physical characterization techniques like scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, energy-dispersive X-ray analysis (EDX), and thermogravimetric analysis (TGA) were employed for the poly(bisphenol Z oxalate)-MCC-magnetite composite during different stages of its formation. The results confirmed the successful modification of the polymer surface. On testing in the presence of three types of binding buffers, a high value of 72.4% (out of 10,000 ng/μL) efficiency with a total yield of DNA at ng and absorbance ratio of A260/A280 (1.980) was observed for the 2 M GuHCl/EtOH binding buffer. These results were compared against the other two buffers of phosphate-buffered saline (PBS) and NaCl. The lowest value of DNA extraction efficiency at 8125 ng/μL of 58.845% with absorbance ratios of A260/A280 (1.818) for PBS was also observed. The study has concluded an enhancement in the DNA extraction efficiency when the polymer is in the composite stage along with cellulose and magnetite particles as compared against the bare polymer. Hindawi Limited 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80064/1/High-efficiency%20DNA%20extraction%20using%20poly%284%2C4%E2%80%B2-cyclohexylidene%20bisphenol%20oxalate%29-modified%20microcrystalline%20cellulose%20magnetite%20composite.pdf Al Balawi, Aisha Nawaf and Yusof, Nor Azah and Kamaruzaman, Sazlinda and Mohammad, Faruq and Wasoh @ Mohamad Isa, Helmi and Al Lohedan, Hamad A. (2019) High-efficiency DNA extraction using poly(4,4′-cyclohexylidene bisphenol oxalate)-modified microcrystalline cellulose magnetite composite. International Journal of Polymer Science. art. no. 5738613. pp. 1-10. ISSN 1687-9422; ESSN: 1687-9430 https://www.hindawi.com/journals/ijps/2019/5738613/ 10.1155/2019/5738613 |
| spellingShingle | Al Balawi, Aisha Nawaf Yusof, Nor Azah Kamaruzaman, Sazlinda Mohammad, Faruq Wasoh @ Mohamad Isa, Helmi Al Lohedan, Hamad A. High-efficiency DNA extraction using poly(4,4′-cyclohexylidene bisphenol oxalate)-modified microcrystalline cellulose magnetite composite |
| title | High-efficiency DNA extraction using poly(4,4′-cyclohexylidene bisphenol oxalate)-modified microcrystalline cellulose magnetite composite |
| title_full | High-efficiency DNA extraction using poly(4,4′-cyclohexylidene bisphenol oxalate)-modified microcrystalline cellulose magnetite composite |
| title_fullStr | High-efficiency DNA extraction using poly(4,4′-cyclohexylidene bisphenol oxalate)-modified microcrystalline cellulose magnetite composite |
| title_full_unstemmed | High-efficiency DNA extraction using poly(4,4′-cyclohexylidene bisphenol oxalate)-modified microcrystalline cellulose magnetite composite |
| title_short | High-efficiency DNA extraction using poly(4,4′-cyclohexylidene bisphenol oxalate)-modified microcrystalline cellulose magnetite composite |
| title_sort | high-efficiency dna extraction using poly(4,4′-cyclohexylidene bisphenol oxalate)-modified microcrystalline cellulose magnetite composite |
| url | http://psasir.upm.edu.my/id/eprint/80064/ http://psasir.upm.edu.my/id/eprint/80064/ http://psasir.upm.edu.my/id/eprint/80064/ http://psasir.upm.edu.my/id/eprint/80064/1/High-efficiency%20DNA%20extraction%20using%20poly%284%2C4%E2%80%B2-cyclohexylidene%20bisphenol%20oxalate%29-modified%20microcrystalline%20cellulose%20magnetite%20composite.pdf |