Genetically-modified human mesenchymal stem cells to express erythropoietin enhances differentiation into retinal photoreceptors: an in-vitro study
Dysfunctional or death of retinal photoreceptors is an irreversible phenomenon that is closely associated with a broad range of retinal degenerative diseases, such as retinitis pigmentosa and age-related macular degeneration (AMD), resulting in successive loss of visual function and blindness. In se...
| Main Authors: | , , , , , |
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| Format: | Article |
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Elsevier BV
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/79987/ |
| _version_ | 1848858802941591552 |
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| author | Lee, Suet Shirley Ding Ee-Hwan, Avin Koh Kumar, Suresh Ali Khan, Mohammed Safwan Alzahrani, Badr Mok, Pooi Ling |
| author_facet | Lee, Suet Shirley Ding Ee-Hwan, Avin Koh Kumar, Suresh Ali Khan, Mohammed Safwan Alzahrani, Badr Mok, Pooi Ling |
| author_sort | Lee, Suet Shirley Ding |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Dysfunctional or death of retinal photoreceptors is an irreversible phenomenon that is closely associated with a broad range of retinal degenerative diseases, such as retinitis pigmentosa and age-related macular degeneration (AMD), resulting in successive loss of visual function and blindness. In search for viable treatment for retinal degenerative diseases, mesenchymal stem cells (MSCs) has demonstrated promising therapeutic capabilities to repair and replace damaged photoreceptor cells in both in vitro and in vivo conditions. Nevertheless, the dearth of MSC differentiation capacity into photoreceptors has limited its use in cell replacement therapy. Erythropoietin (EPO) has vital role in early neural retinal cell differentiation and demonstrated rescue potential on dying photoreceptor cells. Hence, we aimed to evaluate the differentiation capacity of MSCs into photoreceptor cells in the presence of human EPO protein. We derived the MSC from human Wharton's jelly of umbilical cord and transduced the cells with lentivirus particles encoding EPO and green fluorescent protein (GFP) as reporter gene. The transduced cells were selectively cultured and induced to differentiate into photoreceptors by exposing to photoreceptor differentiation cocktail. Our preliminary results showed that transduced cells exposed to induction medium had an enhanced differentiation capacity when compared to non-transduced cells. Our results demonstrated a novel strategy to increase the yield of in vitro photoreceptor differentiation and may be potentially useful in improving the efficiency of stem cell transplantation for ocular disorders. |
| first_indexed | 2025-11-15T12:19:14Z |
| format | Article |
| id | upm-79987 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T12:19:14Z |
| publishDate | 2019 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-799872023-05-02T02:39:20Z http://psasir.upm.edu.my/id/eprint/79987/ Genetically-modified human mesenchymal stem cells to express erythropoietin enhances differentiation into retinal photoreceptors: an in-vitro study Lee, Suet Shirley Ding Ee-Hwan, Avin Koh Kumar, Suresh Ali Khan, Mohammed Safwan Alzahrani, Badr Mok, Pooi Ling Dysfunctional or death of retinal photoreceptors is an irreversible phenomenon that is closely associated with a broad range of retinal degenerative diseases, such as retinitis pigmentosa and age-related macular degeneration (AMD), resulting in successive loss of visual function and blindness. In search for viable treatment for retinal degenerative diseases, mesenchymal stem cells (MSCs) has demonstrated promising therapeutic capabilities to repair and replace damaged photoreceptor cells in both in vitro and in vivo conditions. Nevertheless, the dearth of MSC differentiation capacity into photoreceptors has limited its use in cell replacement therapy. Erythropoietin (EPO) has vital role in early neural retinal cell differentiation and demonstrated rescue potential on dying photoreceptor cells. Hence, we aimed to evaluate the differentiation capacity of MSCs into photoreceptor cells in the presence of human EPO protein. We derived the MSC from human Wharton's jelly of umbilical cord and transduced the cells with lentivirus particles encoding EPO and green fluorescent protein (GFP) as reporter gene. The transduced cells were selectively cultured and induced to differentiate into photoreceptors by exposing to photoreceptor differentiation cocktail. Our preliminary results showed that transduced cells exposed to induction medium had an enhanced differentiation capacity when compared to non-transduced cells. Our results demonstrated a novel strategy to increase the yield of in vitro photoreceptor differentiation and may be potentially useful in improving the efficiency of stem cell transplantation for ocular disorders. Elsevier BV 2019 Article PeerReviewed Lee, Suet Shirley Ding and Ee-Hwan, Avin Koh and Kumar, Suresh and Ali Khan, Mohammed Safwan and Alzahrani, Badr and Mok, Pooi Ling (2019) Genetically-modified human mesenchymal stem cells to express erythropoietin enhances differentiation into retinal photoreceptors: an in-vitro study. Journal of Photochemistry and Photobiology, B: Biology, 195. pp. 33-38. ISSN 1011-1344; ESSN: 1873-2682 https://www.sciencedirect.com/science/article/pii/S1011134418306286?via%3Dihub 10.1016/j.jphotobiol.2019.04.008 |
| spellingShingle | Lee, Suet Shirley Ding Ee-Hwan, Avin Koh Kumar, Suresh Ali Khan, Mohammed Safwan Alzahrani, Badr Mok, Pooi Ling Genetically-modified human mesenchymal stem cells to express erythropoietin enhances differentiation into retinal photoreceptors: an in-vitro study |
| title | Genetically-modified human mesenchymal stem cells to express erythropoietin enhances differentiation into retinal photoreceptors: an in-vitro study |
| title_full | Genetically-modified human mesenchymal stem cells to express erythropoietin enhances differentiation into retinal photoreceptors: an in-vitro study |
| title_fullStr | Genetically-modified human mesenchymal stem cells to express erythropoietin enhances differentiation into retinal photoreceptors: an in-vitro study |
| title_full_unstemmed | Genetically-modified human mesenchymal stem cells to express erythropoietin enhances differentiation into retinal photoreceptors: an in-vitro study |
| title_short | Genetically-modified human mesenchymal stem cells to express erythropoietin enhances differentiation into retinal photoreceptors: an in-vitro study |
| title_sort | genetically-modified human mesenchymal stem cells to express erythropoietin enhances differentiation into retinal photoreceptors: an in-vitro study |
| url | http://psasir.upm.edu.my/id/eprint/79987/ http://psasir.upm.edu.my/id/eprint/79987/ http://psasir.upm.edu.my/id/eprint/79987/ |