Experimental characterization of the chronic constriction injury-induced neuropathic pain model in mice
Number of ligations made in the chronic constriction injury (CCI) neuropathic pain model has raised serious concerns. We compared behavioural responses, nerve morphology and expression of pain marker, c-fos among CCI models developed with one, two, three and four ligations. The numbers of ligation(s...
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| Format: | Article |
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Springer
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/79797/ |
| _version_ | 1848858761679077376 |
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| author | Gopalsamy, Banulata Sambasevam, Yogesvari Zulazmi, Nurul Atiqah Chia, Jasmine Siew Min Farouk, Ahmad Akira Omar Sulaiman, Mohd Roslan Tengku Mohamad, Tengku Azam Shah Perimal, Enoch Kumar |
| author_facet | Gopalsamy, Banulata Sambasevam, Yogesvari Zulazmi, Nurul Atiqah Chia, Jasmine Siew Min Farouk, Ahmad Akira Omar Sulaiman, Mohd Roslan Tengku Mohamad, Tengku Azam Shah Perimal, Enoch Kumar |
| author_sort | Gopalsamy, Banulata |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Number of ligations made in the chronic constriction injury (CCI) neuropathic pain model has raised serious concerns. We compared behavioural responses, nerve morphology and expression of pain marker, c-fos among CCI models developed with one, two, three and four ligations. The numbers of ligation(s) on sciatic nerve shows no significant difference in displaying mechanical and cold allodynia, and mechanical and thermal hyperalgesia throughout 84 days. All groups underwent similar levels of nerve degeneration post-surgery. Similar c-fos level in brain cingulate cortex, parafascicular nuclei and amygdala were observed in all CCI models compared to sham-operated group. Therefore, number of ligations does not impact intensity of pain symptoms, pathogenesis and neuronal activation. A single ligation is sufficient to develop neuropathic pain, in contrast to the established model of four ligations. This study dissects and characterises the CCI model, ascertaining a more uniform animal model to surrogate actual neuropathic pain condition. |
| first_indexed | 2025-11-15T12:18:35Z |
| format | Article |
| id | upm-79797 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T12:18:35Z |
| publishDate | 2019 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-797972022-11-07T03:56:08Z http://psasir.upm.edu.my/id/eprint/79797/ Experimental characterization of the chronic constriction injury-induced neuropathic pain model in mice Gopalsamy, Banulata Sambasevam, Yogesvari Zulazmi, Nurul Atiqah Chia, Jasmine Siew Min Farouk, Ahmad Akira Omar Sulaiman, Mohd Roslan Tengku Mohamad, Tengku Azam Shah Perimal, Enoch Kumar Number of ligations made in the chronic constriction injury (CCI) neuropathic pain model has raised serious concerns. We compared behavioural responses, nerve morphology and expression of pain marker, c-fos among CCI models developed with one, two, three and four ligations. The numbers of ligation(s) on sciatic nerve shows no significant difference in displaying mechanical and cold allodynia, and mechanical and thermal hyperalgesia throughout 84 days. All groups underwent similar levels of nerve degeneration post-surgery. Similar c-fos level in brain cingulate cortex, parafascicular nuclei and amygdala were observed in all CCI models compared to sham-operated group. Therefore, number of ligations does not impact intensity of pain symptoms, pathogenesis and neuronal activation. A single ligation is sufficient to develop neuropathic pain, in contrast to the established model of four ligations. This study dissects and characterises the CCI model, ascertaining a more uniform animal model to surrogate actual neuropathic pain condition. Springer 2019 Article PeerReviewed Gopalsamy, Banulata and Sambasevam, Yogesvari and Zulazmi, Nurul Atiqah and Chia, Jasmine Siew Min and Farouk, Ahmad Akira Omar and Sulaiman, Mohd Roslan and Tengku Mohamad, Tengku Azam Shah and Perimal, Enoch Kumar (2019) Experimental characterization of the chronic constriction injury-induced neuropathic pain model in mice. Neurochemical Research, 44 (9). pp. 2123-2138. ISSN 0364-3190; ESSN: 1573-6903 https://link.springer.com/article/10.1007/s11064-019-02850-0 10.1007/s11064-019-02850-0 |
| spellingShingle | Gopalsamy, Banulata Sambasevam, Yogesvari Zulazmi, Nurul Atiqah Chia, Jasmine Siew Min Farouk, Ahmad Akira Omar Sulaiman, Mohd Roslan Tengku Mohamad, Tengku Azam Shah Perimal, Enoch Kumar Experimental characterization of the chronic constriction injury-induced neuropathic pain model in mice |
| title | Experimental characterization of the chronic constriction injury-induced neuropathic pain model in mice |
| title_full | Experimental characterization of the chronic constriction injury-induced neuropathic pain model in mice |
| title_fullStr | Experimental characterization of the chronic constriction injury-induced neuropathic pain model in mice |
| title_full_unstemmed | Experimental characterization of the chronic constriction injury-induced neuropathic pain model in mice |
| title_short | Experimental characterization of the chronic constriction injury-induced neuropathic pain model in mice |
| title_sort | experimental characterization of the chronic constriction injury-induced neuropathic pain model in mice |
| url | http://psasir.upm.edu.my/id/eprint/79797/ http://psasir.upm.edu.my/id/eprint/79797/ http://psasir.upm.edu.my/id/eprint/79797/ |