Evaluation of ultra-microscopic changes and proliferation of apoptotic glioblastoma multiforme cells induced by velogenic strain of Newcastle disease virus AF2240
Aim: Newcastle disease virus (NDV) is a member of genus Avulavirus within the family Paramyxoviridae. Interest of using NDV as an anticancer agent has arisen from its ability to kill tumor cells with limited toxicity to normal cells. Methods: In this investigation, the proliferation of brain tumor c...
| Main Authors: | , , , , , |
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| Format: | Article |
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West Asia Organization for Cancer Prevention
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/79780/ |
| _version_ | 1848858757672468480 |
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| author | Saeed, Rola Ali Alabsi, Aied M. Ideris, Aini Omar, Abdul Rahman Yusoff, Khatijah Ali, Abdul Manaf |
| author_facet | Saeed, Rola Ali Alabsi, Aied M. Ideris, Aini Omar, Abdul Rahman Yusoff, Khatijah Ali, Abdul Manaf |
| author_sort | Saeed, Rola Ali |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Aim: Newcastle disease virus (NDV) is a member of genus Avulavirus within the family Paramyxoviridae. Interest of using NDV as an anticancer agent has arisen from its ability to kill tumor cells with limited toxicity to normal cells. Methods: In this investigation, the proliferation of brain tumor cell line, glioblastoma multiform (DBTRG.05MG) induced by NDV strain AF2240 was evaluated in-vitro, by using MTT proliferation assay. Furthermore, Cytological observations were studied using fluorescence microscopy and transmission electron microscopy, DNA laddering in agarose gel electrophoresis assay used to detect the mode of cell death and analysis of the cellular DNA content by flowcytometery. Results: MTT proliferation assay, Cytological observations using fluorescence microscopy and transmission electron microscopy show the anti-proliferation effect and apoptogenic features of NDV on DBTRG.05MG. Furthermore, analysis of the cellular DNA content showed that there was a loss of treated cells in all cell cycle phases (G1, S and G2/M) accompanied with increasing in sub-G1 region (apoptosis peak). Conclusion: It could be concluded that NDV strain AF2240 is a potent antitumor agent that induce apoptosis and its cytotoxicity increasing while increasing of time and virus titer. |
| first_indexed | 2025-11-15T12:18:31Z |
| format | Article |
| id | upm-79780 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T12:18:31Z |
| publishDate | 2019 |
| publisher | West Asia Organization for Cancer Prevention |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-797802022-11-04T12:37:29Z http://psasir.upm.edu.my/id/eprint/79780/ Evaluation of ultra-microscopic changes and proliferation of apoptotic glioblastoma multiforme cells induced by velogenic strain of Newcastle disease virus AF2240 Saeed, Rola Ali Alabsi, Aied M. Ideris, Aini Omar, Abdul Rahman Yusoff, Khatijah Ali, Abdul Manaf Aim: Newcastle disease virus (NDV) is a member of genus Avulavirus within the family Paramyxoviridae. Interest of using NDV as an anticancer agent has arisen from its ability to kill tumor cells with limited toxicity to normal cells. Methods: In this investigation, the proliferation of brain tumor cell line, glioblastoma multiform (DBTRG.05MG) induced by NDV strain AF2240 was evaluated in-vitro, by using MTT proliferation assay. Furthermore, Cytological observations were studied using fluorescence microscopy and transmission electron microscopy, DNA laddering in agarose gel electrophoresis assay used to detect the mode of cell death and analysis of the cellular DNA content by flowcytometery. Results: MTT proliferation assay, Cytological observations using fluorescence microscopy and transmission electron microscopy show the anti-proliferation effect and apoptogenic features of NDV on DBTRG.05MG. Furthermore, analysis of the cellular DNA content showed that there was a loss of treated cells in all cell cycle phases (G1, S and G2/M) accompanied with increasing in sub-G1 region (apoptosis peak). Conclusion: It could be concluded that NDV strain AF2240 is a potent antitumor agent that induce apoptosis and its cytotoxicity increasing while increasing of time and virus titer. West Asia Organization for Cancer Prevention 2019 Article PeerReviewed Saeed, Rola Ali and Alabsi, Aied M. and Ideris, Aini and Omar, Abdul Rahman and Yusoff, Khatijah and Ali, Abdul Manaf (2019) Evaluation of ultra-microscopic changes and proliferation of apoptotic glioblastoma multiforme cells induced by velogenic strain of Newcastle disease virus AF2240. Asian Pacific Journal of Cancer Prevention, 20 (3). pp. 757-765. ISSN 1513-7368 http://journal.waocp.org/?sid=Entrez:PubMed&id=pmid:30909682&key=2019.20.3.757 10.31557/APJCP.2019.20.3.757 |
| spellingShingle | Saeed, Rola Ali Alabsi, Aied M. Ideris, Aini Omar, Abdul Rahman Yusoff, Khatijah Ali, Abdul Manaf Evaluation of ultra-microscopic changes and proliferation of apoptotic glioblastoma multiforme cells induced by velogenic strain of Newcastle disease virus AF2240 |
| title | Evaluation of ultra-microscopic changes and proliferation of apoptotic glioblastoma multiforme cells induced by velogenic strain of Newcastle disease virus AF2240 |
| title_full | Evaluation of ultra-microscopic changes and proliferation of apoptotic glioblastoma multiforme cells induced by velogenic strain of Newcastle disease virus AF2240 |
| title_fullStr | Evaluation of ultra-microscopic changes and proliferation of apoptotic glioblastoma multiforme cells induced by velogenic strain of Newcastle disease virus AF2240 |
| title_full_unstemmed | Evaluation of ultra-microscopic changes and proliferation of apoptotic glioblastoma multiforme cells induced by velogenic strain of Newcastle disease virus AF2240 |
| title_short | Evaluation of ultra-microscopic changes and proliferation of apoptotic glioblastoma multiforme cells induced by velogenic strain of Newcastle disease virus AF2240 |
| title_sort | evaluation of ultra-microscopic changes and proliferation of apoptotic glioblastoma multiforme cells induced by velogenic strain of newcastle disease virus af2240 |
| url | http://psasir.upm.edu.my/id/eprint/79780/ http://psasir.upm.edu.my/id/eprint/79780/ http://psasir.upm.edu.my/id/eprint/79780/ |