Effects of aluminium on Clarias gariepinus physiology, gills histology and cholinesterase in vivo and in vitro
The gill is the site of acid-base balance, ionic regulation, gas exchange, and nitrogenous waste excretion by fish. The physiological, histopathological and enzymatic alterations from Clarias gariepinus gills were examined to assess the effects induced in gill tissues within 96 h exposure to waterbo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Smith and Franklin Academic Publishing
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/79574/ http://psasir.upm.edu.my/id/eprint/79574/1/Effects%20of%20aluminium%20on%20Clarias%20gariepinus%20physiology.pdf |
| _version_ | 1848858711358963712 |
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| author | Fadzil, Nadia Izati Shukor, Mohd Yunus Sabullah, Mohd Khalizan Mohd Daud, Hassan Zulkharnain, Azham Ahmad, Siti Aqlima |
| author_facet | Fadzil, Nadia Izati Shukor, Mohd Yunus Sabullah, Mohd Khalizan Mohd Daud, Hassan Zulkharnain, Azham Ahmad, Siti Aqlima |
| author_sort | Fadzil, Nadia Izati |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The gill is the site of acid-base balance, ionic regulation, gas exchange, and nitrogenous waste excretion by fish. The physiological, histopathological and enzymatic alterations from Clarias gariepinus gills were examined to assess the effects induced in gill tissues within 96 h exposure to waterborne Al 2 (SO 4 ) 3 with different concentrations (25 to 300 mg/L). The exposure of C. gariepinus to aluminium showed deleterious effects to normal fish behaviours and varying degrees of gill damage when visualised under inverted light microscopy (stained with haematoxylin-eosin), scanning electron microscopy and transmission electron microscopy. The mortality rate was observed increasing with increased Al concentration showing noticeably disrupted lamellae, irregular nucleus shape and increasing area of vacuolation in treated fish gills associated with Al 2 (SO 4 ) 3 exposure concentration. Furthermore, electron microscopic X-ray microanalysis of fish gills exposed to sublethal Al revealed Al accumulated on the surface of the gill lamella. Al toxicity also gave inhibitory effects on cholinesterase (ChE) extracted from the fish gills with 90.0% inhibition at 300 mg/L Al highest concentration exposure (in vivo), whereas the Al metal ion inhibited 50% of ChE activity at 4.12 mg/L (IC 50 ). |
| first_indexed | 2025-11-15T12:17:47Z |
| format | Article |
| id | upm-79574 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:17:47Z |
| publishDate | 2019 |
| publisher | Smith and Franklin Academic Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-795742021-06-29T23:58:48Z http://psasir.upm.edu.my/id/eprint/79574/ Effects of aluminium on Clarias gariepinus physiology, gills histology and cholinesterase in vivo and in vitro Fadzil, Nadia Izati Shukor, Mohd Yunus Sabullah, Mohd Khalizan Mohd Daud, Hassan Zulkharnain, Azham Ahmad, Siti Aqlima The gill is the site of acid-base balance, ionic regulation, gas exchange, and nitrogenous waste excretion by fish. The physiological, histopathological and enzymatic alterations from Clarias gariepinus gills were examined to assess the effects induced in gill tissues within 96 h exposure to waterborne Al 2 (SO 4 ) 3 with different concentrations (25 to 300 mg/L). The exposure of C. gariepinus to aluminium showed deleterious effects to normal fish behaviours and varying degrees of gill damage when visualised under inverted light microscopy (stained with haematoxylin-eosin), scanning electron microscopy and transmission electron microscopy. The mortality rate was observed increasing with increased Al concentration showing noticeably disrupted lamellae, irregular nucleus shape and increasing area of vacuolation in treated fish gills associated with Al 2 (SO 4 ) 3 exposure concentration. Furthermore, electron microscopic X-ray microanalysis of fish gills exposed to sublethal Al revealed Al accumulated on the surface of the gill lamella. Al toxicity also gave inhibitory effects on cholinesterase (ChE) extracted from the fish gills with 90.0% inhibition at 300 mg/L Al highest concentration exposure (in vivo), whereas the Al metal ion inhibited 50% of ChE activity at 4.12 mg/L (IC 50 ). Smith and Franklin Academic Publishing 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/79574/1/Effects%20of%20aluminium%20on%20Clarias%20gariepinus%20physiology.pdf Fadzil, Nadia Izati and Shukor, Mohd Yunus and Sabullah, Mohd Khalizan and Mohd Daud, Hassan and Zulkharnain, Azham and Ahmad, Siti Aqlima (2019) Effects of aluminium on Clarias gariepinus physiology, gills histology and cholinesterase in vivo and in vitro. International Journal of Agriculture and Biology, 22 (1). pp. 91-101. ISSN 1560-8530; ESSN: 1814-9596 https://shibaura.pure.elsevier.com/en/publications/effects-of-aluminium-on-clarias-gariepinus-physiology-gills-histo |
| spellingShingle | Fadzil, Nadia Izati Shukor, Mohd Yunus Sabullah, Mohd Khalizan Mohd Daud, Hassan Zulkharnain, Azham Ahmad, Siti Aqlima Effects of aluminium on Clarias gariepinus physiology, gills histology and cholinesterase in vivo and in vitro |
| title | Effects of aluminium on Clarias gariepinus physiology, gills histology and cholinesterase in vivo and in vitro |
| title_full | Effects of aluminium on Clarias gariepinus physiology, gills histology and cholinesterase in vivo and in vitro |
| title_fullStr | Effects of aluminium on Clarias gariepinus physiology, gills histology and cholinesterase in vivo and in vitro |
| title_full_unstemmed | Effects of aluminium on Clarias gariepinus physiology, gills histology and cholinesterase in vivo and in vitro |
| title_short | Effects of aluminium on Clarias gariepinus physiology, gills histology and cholinesterase in vivo and in vitro |
| title_sort | effects of aluminium on clarias gariepinus physiology, gills histology and cholinesterase in vivo and in vitro |
| url | http://psasir.upm.edu.my/id/eprint/79574/ http://psasir.upm.edu.my/id/eprint/79574/ http://psasir.upm.edu.my/id/eprint/79574/1/Effects%20of%20aluminium%20on%20Clarias%20gariepinus%20physiology.pdf |