Diversity of respiratory viruses detected among hospitalized children with acute lower respiratory tract infections at Hospital Serdang, Malaysia
Background: The role of respiratory viruses as the major cause of acute lower respiratory tract infections (ALRTIs) in children is becoming increasingly evident due to the use of sensitive molecular detection methods. The aim of this study was to use conventional and molecular detection methods to a...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/79420/ http://psasir.upm.edu.my/id/eprint/79420/1/Diversity%20of%20respiratory%20viruses%20.pdf |
| _version_ | 1848858673767514112 |
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| author | Etemadi, Mohammad Reza Jalilian, Farid Azizi Othman, Norlijah Lye, Munn Sann Ansari, Sara Yubbu, Putri Sekawi, Zamberi |
| author_facet | Etemadi, Mohammad Reza Jalilian, Farid Azizi Othman, Norlijah Lye, Munn Sann Ansari, Sara Yubbu, Putri Sekawi, Zamberi |
| author_sort | Etemadi, Mohammad Reza |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: The role of respiratory viruses as the major cause of acute lower respiratory tract infections (ALRTIs) in children is becoming increasingly evident due to the use of sensitive molecular detection methods. The aim of this study was to use conventional and molecular detection methods to assess the epidemiology of respiratory viral infections in children less than five years of age that were hospitalized with ALRTIs. Methods: The cross-sectional study was designed to investigate the occurrence of respiratory viruses including respiratory syncytisl virus (RSV), human metapneumovirus (HMPV), influenza virus A and B (IFV-A and B), parainfluenzavirus 1, 2, 3 and 4 (PIV 1, 2, 3 and 4), human rhinoviruses (HRV), human enterovirus (HEV), human coronaviruses (HCoV) 229E and OC43, human bocavirus (HBoV) and human adenovirus (HAdV) in hospitalized children with ALRTIs, at Hospital Serdang, Malaysia, from June 16 to December 21, 2009. The study was also designed in part to assess the performance of the conventional methods against molecular methods. Results: Viral pathogens were detected in 158 (95.8%) of the patients. Single virus infections were detected in 114 (67.9%) patients; 46 (27.9%) were co-infected with different viruses including double-virus infections in 37 (22.4%) and triple-virus infections in 9 (5.5%) cases. Approximately 70% of samples were found to be positive using conventional methods compared with 96% using molecular methods. A wide range of respiratory viruses were detected in the study. There was a high prevalence of RSV (50.3%) infections, particularly group B viruses. Other etiological agents including HAdV, HMPV, IFV-A, PIV 1-3, HBoV, HCoV-OC43 and HEV were detected in 14.5, 9.6, 9.1, 4.8, 3.6, 2.4 and 1.8 percent of the samples, respectively. Conclusion: Our results demonstrated the increased sensitivity of molecular detection methods compared with conventional methods for the diagnosis of ARTIs in hospitalized children. This is the first report of HMPV infections in Malaysia. |
| first_indexed | 2025-11-15T12:17:11Z |
| format | Article |
| id | upm-79420 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:17:11Z |
| publishDate | 2019 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-794202021-04-05T06:42:38Z http://psasir.upm.edu.my/id/eprint/79420/ Diversity of respiratory viruses detected among hospitalized children with acute lower respiratory tract infections at Hospital Serdang, Malaysia Etemadi, Mohammad Reza Jalilian, Farid Azizi Othman, Norlijah Lye, Munn Sann Ansari, Sara Yubbu, Putri Sekawi, Zamberi Background: The role of respiratory viruses as the major cause of acute lower respiratory tract infections (ALRTIs) in children is becoming increasingly evident due to the use of sensitive molecular detection methods. The aim of this study was to use conventional and molecular detection methods to assess the epidemiology of respiratory viral infections in children less than five years of age that were hospitalized with ALRTIs. Methods: The cross-sectional study was designed to investigate the occurrence of respiratory viruses including respiratory syncytisl virus (RSV), human metapneumovirus (HMPV), influenza virus A and B (IFV-A and B), parainfluenzavirus 1, 2, 3 and 4 (PIV 1, 2, 3 and 4), human rhinoviruses (HRV), human enterovirus (HEV), human coronaviruses (HCoV) 229E and OC43, human bocavirus (HBoV) and human adenovirus (HAdV) in hospitalized children with ALRTIs, at Hospital Serdang, Malaysia, from June 16 to December 21, 2009. The study was also designed in part to assess the performance of the conventional methods against molecular methods. Results: Viral pathogens were detected in 158 (95.8%) of the patients. Single virus infections were detected in 114 (67.9%) patients; 46 (27.9%) were co-infected with different viruses including double-virus infections in 37 (22.4%) and triple-virus infections in 9 (5.5%) cases. Approximately 70% of samples were found to be positive using conventional methods compared with 96% using molecular methods. A wide range of respiratory viruses were detected in the study. There was a high prevalence of RSV (50.3%) infections, particularly group B viruses. Other etiological agents including HAdV, HMPV, IFV-A, PIV 1-3, HBoV, HCoV-OC43 and HEV were detected in 14.5, 9.6, 9.1, 4.8, 3.6, 2.4 and 1.8 percent of the samples, respectively. Conclusion: Our results demonstrated the increased sensitivity of molecular detection methods compared with conventional methods for the diagnosis of ARTIs in hospitalized children. This is the first report of HMPV infections in Malaysia. Elsevier 2019-07 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/79420/1/Diversity%20of%20respiratory%20viruses%20.pdf Etemadi, Mohammad Reza and Jalilian, Farid Azizi and Othman, Norlijah and Lye, Munn Sann and Ansari, Sara and Yubbu, Putri and Sekawi, Zamberi (2019) Diversity of respiratory viruses detected among hospitalized children with acute lower respiratory tract infections at Hospital Serdang, Malaysia. Journal of Virological Methods, 269. pp. 1-6. ISSN 0166-0934 https://pubmed.ncbi.nlm.nih.gov/30910688/ 10.1016/j.jviromet.2019.03.013 |
| spellingShingle | Etemadi, Mohammad Reza Jalilian, Farid Azizi Othman, Norlijah Lye, Munn Sann Ansari, Sara Yubbu, Putri Sekawi, Zamberi Diversity of respiratory viruses detected among hospitalized children with acute lower respiratory tract infections at Hospital Serdang, Malaysia |
| title | Diversity of respiratory viruses detected among hospitalized children with acute lower respiratory tract infections at Hospital Serdang, Malaysia |
| title_full | Diversity of respiratory viruses detected among hospitalized children with acute lower respiratory tract infections at Hospital Serdang, Malaysia |
| title_fullStr | Diversity of respiratory viruses detected among hospitalized children with acute lower respiratory tract infections at Hospital Serdang, Malaysia |
| title_full_unstemmed | Diversity of respiratory viruses detected among hospitalized children with acute lower respiratory tract infections at Hospital Serdang, Malaysia |
| title_short | Diversity of respiratory viruses detected among hospitalized children with acute lower respiratory tract infections at Hospital Serdang, Malaysia |
| title_sort | diversity of respiratory viruses detected among hospitalized children with acute lower respiratory tract infections at hospital serdang, malaysia |
| url | http://psasir.upm.edu.my/id/eprint/79420/ http://psasir.upm.edu.my/id/eprint/79420/ http://psasir.upm.edu.my/id/eprint/79420/ http://psasir.upm.edu.my/id/eprint/79420/1/Diversity%20of%20respiratory%20viruses%20.pdf |