Direct use of spent mushroom substrate from Pleurotus pulmonarius as a readily delignified feedstock for cellulase production
The feasibility of spent mushroom substrate (SMS) as an alternative fermentation feedstock for cellulase production has been demonstrated in this work. Utilization of SMS as a substrate has been attempted widely due to its high cellulose content and readily available in smaller particle size. On top...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Springer
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/79396/ http://psasir.upm.edu.my/id/eprint/79396/1/Direct%20use%20of%20spent%20mushroom%20substrate%20.pdf |
| _version_ | 1848858668733300736 |
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| author | Mohd Ariff, Iffah Nabilah Kamal Bahrin, Ezyana Ramli, Norhayati Abd. Aziz, Suraini |
| author_facet | Mohd Ariff, Iffah Nabilah Kamal Bahrin, Ezyana Ramli, Norhayati Abd. Aziz, Suraini |
| author_sort | Mohd Ariff, Iffah Nabilah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The feasibility of spent mushroom substrate (SMS) as an alternative fermentation feedstock for cellulase production has been demonstrated in this work. Utilization of SMS as a substrate has been attempted widely due to its high cellulose content and readily available in smaller particle size. On top of that, the availability of delignified SMS by the action of Pleurotus pulmonarius during mushroom cultivation offers another benefit to its use whereby no chemical pretreatment would be required prior to fermentation. The recovery of crude laccase and manganese peroxidase from delignified SMS were found to be 3 and 1.4 U/g, respectively. Further to this, the cellulase production from SMS by Trichoderma asperellum UPM 1 under solid state fermentation was optimized by applying central composite design, resulted in increment of 1.4-fold in CMCase (171.21 U/g) and 1.5-fold in β-glucosidase (6.83 U/g), with the optimum temperature of 27.5 °C, initial moisture content 81% and initial pH of fermentation 4.5. Therefore, this study showed that the direct utilization of SMS is feasible for promising cellulase production by T. asperellum UPM 1. |
| first_indexed | 2025-11-15T12:17:06Z |
| format | Article |
| id | upm-79396 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:17:06Z |
| publishDate | 2019 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-793962021-03-30T23:52:35Z http://psasir.upm.edu.my/id/eprint/79396/ Direct use of spent mushroom substrate from Pleurotus pulmonarius as a readily delignified feedstock for cellulase production Mohd Ariff, Iffah Nabilah Kamal Bahrin, Ezyana Ramli, Norhayati Abd. Aziz, Suraini The feasibility of spent mushroom substrate (SMS) as an alternative fermentation feedstock for cellulase production has been demonstrated in this work. Utilization of SMS as a substrate has been attempted widely due to its high cellulose content and readily available in smaller particle size. On top of that, the availability of delignified SMS by the action of Pleurotus pulmonarius during mushroom cultivation offers another benefit to its use whereby no chemical pretreatment would be required prior to fermentation. The recovery of crude laccase and manganese peroxidase from delignified SMS were found to be 3 and 1.4 U/g, respectively. Further to this, the cellulase production from SMS by Trichoderma asperellum UPM 1 under solid state fermentation was optimized by applying central composite design, resulted in increment of 1.4-fold in CMCase (171.21 U/g) and 1.5-fold in β-glucosidase (6.83 U/g), with the optimum temperature of 27.5 °C, initial moisture content 81% and initial pH of fermentation 4.5. Therefore, this study showed that the direct utilization of SMS is feasible for promising cellulase production by T. asperellum UPM 1. Springer 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/79396/1/Direct%20use%20of%20spent%20mushroom%20substrate%20.pdf Mohd Ariff, Iffah Nabilah and Kamal Bahrin, Ezyana and Ramli, Norhayati and Abd. Aziz, Suraini (2019) Direct use of spent mushroom substrate from Pleurotus pulmonarius as a readily delignified feedstock for cellulase production. Waste and Biomass Valorization, 10 (4). pp. 839-850. ISSN 1877-2641; ESSN: 1877-265X https://link.springer.com/article/10.1007/s12649-017-0106-8 10.1007/s12649-017-0106-8 |
| spellingShingle | Mohd Ariff, Iffah Nabilah Kamal Bahrin, Ezyana Ramli, Norhayati Abd. Aziz, Suraini Direct use of spent mushroom substrate from Pleurotus pulmonarius as a readily delignified feedstock for cellulase production |
| title | Direct use of spent mushroom substrate from Pleurotus pulmonarius as a readily delignified feedstock for cellulase production |
| title_full | Direct use of spent mushroom substrate from Pleurotus pulmonarius as a readily delignified feedstock for cellulase production |
| title_fullStr | Direct use of spent mushroom substrate from Pleurotus pulmonarius as a readily delignified feedstock for cellulase production |
| title_full_unstemmed | Direct use of spent mushroom substrate from Pleurotus pulmonarius as a readily delignified feedstock for cellulase production |
| title_short | Direct use of spent mushroom substrate from Pleurotus pulmonarius as a readily delignified feedstock for cellulase production |
| title_sort | direct use of spent mushroom substrate from pleurotus pulmonarius as a readily delignified feedstock for cellulase production |
| url | http://psasir.upm.edu.my/id/eprint/79396/ http://psasir.upm.edu.my/id/eprint/79396/ http://psasir.upm.edu.my/id/eprint/79396/ http://psasir.upm.edu.my/id/eprint/79396/1/Direct%20use%20of%20spent%20mushroom%20substrate%20.pdf |