Disease agents affecting the eggs and adults of horseshoe crab, Tachypleus gigas (Muller, 1785) and Carcinoscorpius rotundicauda (Latreille, 1802) in laboratory culture
This study was conducted to identify different disease agents affecting the development of eggs and adults horseshoe crabs and to determine the effect of aeration on the horseshoe crab eggs development. Tachypleus gigas and Carcinoscorpius rotundicauda eggs used in this study were incubated using...
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/79270/ http://psasir.upm.edu.my/id/eprint/79270/1/FP%202012%2099%20IR.pdf |
| Summary: | This study was conducted to identify different disease agents affecting the
development of eggs and adults horseshoe crabs and to determine the effect of
aeration on the horseshoe crab eggs development. Tachypleus gigas and
Carcinoscorpius rotundicauda eggs used in this study were incubated using a
conventional culture system. Samples with signs of infection were examined
under a compound microscope and photographed. Eggs inoculations were
prepared and cultured on Sabouraud Dextrose salt Agar for fungal isolation and
TrypticaseTM Soy Broth (TSB) for bacterial isolation. Identification of different
disease agents were conducted based on the morphology of fresh and stained
mounts as well as the biochemical characters of the isolated fungi and bacteria.
Adults of T. gigas and C. rotundicauda were examined similarly. This study was
also conducted to determine the effect of aeration on development of T. gigas
eggs. Eggs incubations in water medium were carried out in two treatments; with
and without aeration. The eggs of T. gigas were infected with Aspergillus sp. and
A. niger, while for C. rotundicauda, the eggs were infected with fungi Aspergillus
niger, Penicillium sp. and Gliocladium sp. The eggs of T. gigas were infected
with bacterial Corynebacterium sp. and Enterococcus faecalis, while
C. rotundicauda eggs were infected with E. faecalis and Shewanella putrefaciens.
As for adult C. rotundicauda, E. faecalis and Bacillus cereus were isolated from
the prosoma and S. putrefaciens was found in the book gills. A trematode worm,
identified as Planaria sp., was found in all parts of the body of adult
C. rotundicauda. Percentage of hatching was found to be significantly lower
(P<0.05) for T. gigas eggs incubated with aeration as compared with those without aeration. |
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