Sequence analysis and expression of VP3 gene of chicken anaemia virus
Chicken anaemia virus (CAV) isolate BL-5 VP3 gene was amplified, sequenced and expressed in E. coli as a fusion protein together with a six-histidine tag. The deduced amino acid sequence of VP3 was identical to other CAV isolates Cux-1, A2 and CIA-1 except for a single amino acid substitution at pos...
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| Format: | Article |
| Language: | English English |
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Veterinary Association Malaysia
2003
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7859/ http://psasir.upm.edu.my/id/eprint/7859/1/Sequence%20analysis%20and%20expression%20of%20VP3%20gene%20of%20chicken%20anemia%20virus.pdf |
| _version_ | 1848840712101036032 |
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| author | M. S., Hasmah Abdul Rahman, Omar Ideris, Aini Bejo, Mohd Hair |
| author_facet | M. S., Hasmah Abdul Rahman, Omar Ideris, Aini Bejo, Mohd Hair |
| author_sort | M. S., Hasmah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Chicken anaemia virus (CAV) isolate BL-5 VP3 gene was amplified, sequenced and expressed in E. coli as a fusion protein together with a six-histidine tag. The deduced amino acid sequence of VP3 was identical to other CAV isolates Cux-1, A2 and CIA-1 except for a single amino acid substitution at position 12. SDS-PAGE and Western blot analysis indicated that the expressed protein is insoluble and was found primarily from the cell lysate fraction. Expression of the protein was detected as early as 1 h, with maximum expression (~12% of the total protein) at 6 h post induction with IPTG. This study indicates that VP3 protein is highly expressed in insoluble form in E. coli. However, the biological function of the protein remains to be studied |
| first_indexed | 2025-11-15T07:31:42Z |
| format | Article |
| id | upm-7859 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:31:42Z |
| publishDate | 2003 |
| publisher | Veterinary Association Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-78592015-10-09T01:47:40Z http://psasir.upm.edu.my/id/eprint/7859/ Sequence analysis and expression of VP3 gene of chicken anaemia virus M. S., Hasmah Abdul Rahman, Omar Ideris, Aini Bejo, Mohd Hair Chicken anaemia virus (CAV) isolate BL-5 VP3 gene was amplified, sequenced and expressed in E. coli as a fusion protein together with a six-histidine tag. The deduced amino acid sequence of VP3 was identical to other CAV isolates Cux-1, A2 and CIA-1 except for a single amino acid substitution at position 12. SDS-PAGE and Western blot analysis indicated that the expressed protein is insoluble and was found primarily from the cell lysate fraction. Expression of the protein was detected as early as 1 h, with maximum expression (~12% of the total protein) at 6 h post induction with IPTG. This study indicates that VP3 protein is highly expressed in insoluble form in E. coli. However, the biological function of the protein remains to be studied Veterinary Association Malaysia 2003 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7859/1/Sequence%20analysis%20and%20expression%20of%20VP3%20gene%20of%20chicken%20anemia%20virus.pdf M. S., Hasmah and Abdul Rahman, Omar and Ideris, Aini and Bejo, Mohd Hair (2003) Sequence analysis and expression of VP3 gene of chicken anaemia virus. Jurnal Veterinar Malaysia, 15 (1-2). pp. 47-51. ISSN 9128-2506 English |
| spellingShingle | M. S., Hasmah Abdul Rahman, Omar Ideris, Aini Bejo, Mohd Hair Sequence analysis and expression of VP3 gene of chicken anaemia virus |
| title | Sequence analysis and expression of VP3 gene of chicken anaemia virus |
| title_full | Sequence analysis and expression of VP3 gene of chicken anaemia virus |
| title_fullStr | Sequence analysis and expression of VP3 gene of chicken anaemia virus |
| title_full_unstemmed | Sequence analysis and expression of VP3 gene of chicken anaemia virus |
| title_short | Sequence analysis and expression of VP3 gene of chicken anaemia virus |
| title_sort | sequence analysis and expression of vp3 gene of chicken anaemia virus |
| url | http://psasir.upm.edu.my/id/eprint/7859/ http://psasir.upm.edu.my/id/eprint/7859/1/Sequence%20analysis%20and%20expression%20of%20VP3%20gene%20of%20chicken%20anemia%20virus.pdf |